Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

Fan, Weisen; Qi, Yinghua; Wang, Yaqian; Yan, Huiting; Li, Xuan; Zhang, Yingjie

doi:10.3389/fcell.2022.1079387

Cited by 5 publications

(3 citation statements)

References 165 publications

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“…EVs released by preimplantation embryos, oviductal, and endometrial cells influence the developmental competence and gene expression of embryos, as well as oviductal/endometrial gene expression and functions [ 137 ]. Since the oviduct and uterus are considered virtual cavities in which embryos develop in confined fluid volumes, EVs may be present at high concentrations suitable for acting as bidirectional players in embryo–maternal tract cell–cell communication to regulate developmental competence and implantation in vivo.…”

Section: Embryo–maternal Tract Interactionsmentioning

confidence: 99%

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Gualtieri,

De Gregorio,

Candela

et al. 2024

Cells

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Preimplantation embryo culture, pivotal in assisted reproductive technology (ART), has lagged in innovation compared to embryo selection advancements. This review examines the persisting gap between in vivo and in vitro embryo development, emphasizing the need for improved culture conditions. While in humans this gap is hardly estimated, animal models, particularly bovines, reveal clear disparities in developmental competence, cryotolerance, pregnancy and live birth rates between in vitro-produced (IVP) and in vivo-derived (IVD) embryos. Molecular analyses unveil distinct differences in morphology, metabolism, and genomic stability, underscoring the need for refining culture conditions for better ART outcomes. To this end, a deeper comprehension of oviduct physiology and embryo transport is crucial for grasping embryo–maternal interactions’ mechanisms. Research on autocrine and paracrine factors, and extracellular vesicles in embryo–maternal tract interactions, elucidates vital communication networks for successful implantation and pregnancy. In vitro, confinement, and embryo density are key factors to boost embryo development. Advanced dynamic culture systems mimicking fluid mechanical stimulation in the oviduct, through vibration, tilting, and microfluidic methods, and the use of innovative softer substrates, hold promise for optimizing in vitro embryo development.

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Section: Embryo–maternal Tract Interactionsmentioning

confidence: 99%

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Gualtieri,

De Gregorio,

Candela

et al. 2024

Cells

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“…Both methods of detection, however, are intrusive and potentially carry risks in the diagnosis of endometriosis. EVs obtained from cervicovaginal lavage and vaginal swabs have been shown in pre-clinical studies to be a novel, minimally invasive method for diagnosing several disorders of the reproductive tract, including endometrial disease [104] . It is speculation that needs additional research to confirm if it is true.…”

Section: Extracellular Vesicle and Endometriosismentioning

confidence: 99%

Breaking the silence: The role of extracellular vesicles in unraveling the diagnosis and treatment of endometriosis

Utkarsh,

Srivastava,

Papayannakos

et al. 2023

Extracell Vesicles Circ Nucleic Acids

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Cell-to-cell communication is believed to be facilitated by membrane-bound vesicles called extracellular vesicles (EVs), which are released by cells. Protein, lipids, and nucleic acids are major cargo of EVs and are transported in these vesicles. Depending on the parent and recipient cell types, they can affect a wide variety of biological processes in the tissues to which they are delivered. EVs are essential for embryo implantation and endometriosis, and they are located in the uterine cavities of different species, where they promote blastocyst and endometrial preparation for implantation. This review focuses on what is currently understood regarding pathologic and diagnostic characteristics, and the potential therapeutic value of EVs in the context of endometriosis, where they can be used for drug delivery and targeted therapy due to their ability to carry bioactive molecules to specific cells or tissues. The findings of this review highlight the potential for a wide range of clinical applications that involve endometrial EVs in the areas of treatment, such as surgical and pharmacological, diagnostic biomarker development, and drug delivery systems, all with the ultimate goal of improving pregnancy success rates.

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“…Extracellular vesicles are bilayered phospholipid membrane structures secreted by all types of cells and containing various bioactive molecules (proteins, RNA, mRNA, mi-croRNA and lipids) [2,3]. According to the recommendations of the International Society…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

Sysoeva,

Akhmedova,

Nepsha

et al. 2024

Life

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The aim of this study was to investigate the molecular composition of follicular fluid (FF) extracellular vesicles (EVs) in women of different reproductive ages and its possible relationship to sperm fertilizing ability. FF EVs were obtained by differential centrifugation. The concentration and size distribution of FF EVs were analyzed by nanoparticle tracking analysis. The lipidome and proteome were analyzed by liquid chromatography–mass spectrometry. The isolated FF EVs had a variety of shapes and sizes; their concentration and size distribution did not differ significantly between the age groups. In women younger than 35 years, the concentration of vesicular progesterone was 6.6 times higher than in women older than 35 years, and the total levels of the main lipid classes were increased in younger women. A proteomic analysis revealed that not only FF EV-specific proteins, but also proteins involved in sperm activation were present. New data were obtained on the composition of FF EVs, confirming their importance as molecular indicators of age-related changes in the female reproductive system. In addition, these results shed light on the possible interaction between the FF EVs of women in different age groups and male germ cells. Therefore, studying the transcriptomic and metabolomic profile of FF EVs may be a crucial approach to evaluate the efficacy of ART.

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Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances

Cited by 5 publications

References 165 publications

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?

Breaking the silence: The role of extracellular vesicles in unraveling the diagnosis and treatment of endometriosis

Characteristics of the Follicular Fluid Extracellular Vesicle Molecular Profile in Women in Different Age Groups in ART Programs

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