Small diamines as modifiers for phosphatidylcholine/phosphatidylserine coatings in capillary electrochromatography

Varjo, Sami; Hautala, Jari T.; Wiedmer, Susanne Κ.; Riekkola, Marja‐Liisa

doi:10.1016/j.chroma.2005.02.006

Cited by 20 publications

(20 citation statements)

References 24 publications

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“…Protein recovery was between 6475 and 9278% indicating some protein adsorption either onto uncoated wall sections or zwitterionic headgroups [206]. Although not applied in protein separations, the addition of diamines improved the stability of PLBs by mediating interaction within a single layer, within the bilayer and between the phospholipids and the silica wall as well [209].…”

Section: Detergentsmentioning

confidence: 99%

Protein attachment onto silica surfaces – a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

2009

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This review addresses the fundamentals governing the adsorption of individual protein molecules onto the surface of fused-silica capillaries, the protein aggregation to adsorbate clusters and their final accretion to monolayers with subsequent stratification to protein multilayers. The attention in CE protein separation has primarily been focused on (i) tuning the BGE including the buffer type, ionic strength, pH and additives, (ii) tailored post-rinse procedures to detach adhered protein residues and (iii) the optimization of capillary wall shielding in order to reduce protein attachment. Improvements in protein separation as well as related adverse effects are mainly discussed on the basis of parameters known to become deteriorated in case of protein adhesion, e.g. repeatability of the EOF and of migration times, peak width, theoretical plate numbers, resolution and asymmetry factor. However, knowledge of the molecular principles controlling protein adsorption onto silica surfaces is indispensable for separation optimization. Furthermore, it facilitates troubleshooting and the interpretation of undesired concomitant phenomena. This review comprehensively discusses protein adsorption models derived from surface chemistry primarily in terms of their relevance for CE, clearly showing that the adsorption process in its complexity is only partially revealed by models, which address single or binary protein solutions. In a further section theoretical concepts and surface models are related to surface phenomena encountered in CE. The final part of the review surveys recent concepts for prevention of protein adhesion, thereby addressing capillary treatment, favorable buffer types, dynamic and adhesive semi-permanent coating strategies covering the literature from 2000-2008.

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Section: Detergentsmentioning

confidence: 99%

Protein attachment onto silica surfaces – a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

2009

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“…The quality of the coating was evaluated via separation of five steroids used as neutral model analytes. Similar to HEPES, addition of small diamines (ethylenediamine, diaminopropane, bis-tris-propane) to the liposome solution improved the coating quality [27] (see Fig. 1).…”

Section: Immobilized Phospholipids As Stationary Phasesmentioning

confidence: 97%

Analysis of liposomes by capillary electrophoresis and their use as carrier in electrokinetic chromatography

Bilek

Kremser

Blaas

et al. 2006

Journal of Chromatography B

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“…The result indicated that hydrophobic interaction between analytes and the phospholipid coating has an effect on the migration of these analytes. Varjo et al [35] found that stability of liposome coatings of PC/PS can be enhanced by adding small diamines (ethylenediamine, diaminopropane, bis-trispropane and so on) to the liposome solution before coating of fused-silica capillaries. The results showed that the small linear diamines increased the adsorbed density of anionic phospholipids, and led to better separation of five neutral steroids under the optimal conditions.…”

Section: Liposomesmentioning

confidence: 99%

Recent progress in polar stationary phases for CEC

Dong

et al. 2007

Electrophoresis

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This review summarizes most of the recent developments in the preparation and application of polar stationary phases for CEC covering the literature published since the year 2004. These polar stationary phases have been adopted for separation of analytes by the modes of packing column CEC, open-tubular CEC (o-CEC) and monolithic column CEC. Currently, development of o-CEC using biomolecules, such as protein and DNA, as the immobilized ligands is highlighted partly due to the simplicity of preparation. Furthermore, monolithic columns have been extended quickly, particularly inorganic materials-based monoliths, such as silica, zirconia, hafnium, etc., as an alternative to packed columns have been developed quickly.

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Small diamines as modifiers for phosphatidylcholine/phosphatidylserine coatings in capillary electrochromatography

Cited by 20 publications

References 24 publications

Protein attachment onto silica surfaces – a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

Protein attachment onto silica surfaces – a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

Analysis of liposomes by capillary electrophoresis and their use as carrier in electrokinetic chromatography

Recent progress in polar stationary phases for CEC

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