SKRINING FITOKIMIA DAN PROFIL KLT METABOLIT SEKUNDER DARI DAUN RUKU-RUKU (Ocimum tenulflorum L.) DAN DAUN KEMANGI (Ocimum sanctum L)

Sopianti, Densi Selpia

doi:10.36434/scientia.v8i1.118

Cited by 8 publications

(9 citation statements)

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“…A total of 0.05 g of extract was blended with 2 mL of ethanol 96%, then added of magnesium chips and 1 mL of concentrated HCl solution. The presence of flavonoids is indicated by brick-red or orange color of solution (Sopianti & Sary, 2018).…”

Section: Methodsmentioning

confidence: 99%

Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta

Aditiyarini

Restiani²,

Evieyana³

2022

bio

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Section: Methodsmentioning

confidence: 99%

Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta

Aditiyarini

Restiani²,

Evieyana³

2022

bio

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“…The filtrate was divided into two parts, the first part was added by Mayer reagent and the second part was added by Wagner reaction. If yellowish-white precipitation formed with Mayer reagent and reddish-brown precipitation formed with Wagner reagent, then the ethanolic extract of pandan tikar leaves is positive for alkaloids (Yadav & Agarwala, 2011 ;Sopianti & Sary, 2018).…”

Section: A Alkaloid Identificationmentioning

confidence: 99%

“…About 0.5 g ethanolic extract of pandan tikar leaves was putted into a reaction tube, and added 2 mL of NaOH 2% solution. If the color is changed into deep yellow which vanished when diluted with adding HCl, then the ethanolic extract of pandan tikar leaves is positive for flavonoids (Yadav & Agarwala, 2011;Sopianti & Sary, 2018).…”

Section: B Flavonoid Identificationmentioning

confidence: 99%

“…Next, added 20 ml of distilled water and shaken vigorously. If the foam is formed then the extract of pandan tikar leaves is positive for saponins (Sopianti & Sary, 2018) e. Steroid identification About 0.5 g ethanolic extract of pandan tikar leaves was putted into a reaction tube, and dissolved with ethanol, added 2 mL chloroform and 2 mL concentrated H2SO4 slowly through the inner side of the reaction tube. If a red ring is formed, then the extract of pandan tikar leaves is positive for steroids (Yadav & Agarwala, 2011;Sopianti & Sary, 2018).…”

Section: Saponin Identificationmentioning

confidence: 99%

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Antidiabetic Activity of Ethanolic Extract of Pandan Tikar (Pandanus tectorius) on Alloxan-Induced Diabetic White Male Rats

Rame

Mbulang

2022

JFG

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Background: Diabetes mellitus is a chronic disease caused by the body's incapacity to use insulin or the inability of the pancreas to produce insulin. Pandan tikar falls under pandanaceae which known for its benefits. Chemical compounds in pandan tikar leaves showed that it has a lot of medicinal activities, one of them is to lower glucose blood level in diabetes mellitus. Object: This study aimed to understand the activity of ethanolic extract of pandan tikar leaves as antidiabetic agent in white male rats which induced by alloxan. Material and Methods: This study used a pretest-posttest design, using 25 Wistar white male rats. Then the rats were divided into five groups, each consisting of 5 rats. Group I, a positive control, was given glibenclamide with a dosage of 0.45 mg/kg BW. For the negative control, Group II, Na-CMC 1% was used. The test groups were Group III, IV, and V, and each received an ethanolic extract of pandan tikar leaves with various dosages. Group III received a dosage of 125 mg/kg BW, group IV 250 mg/kg BW, and group V 375 mg/kg BW. The rats were conditioned in diabetic stage by using alloxan 150 mg/kg bw as an inducer, given intraperitoneally, then each rats treated accordingly to their own group for 14 days. The determination of blood glucose level was done enzymatically using glucometer. Result: The result showed that ethanolic extract of pandan tikar leaves able to lower the glucose blood level and not significantly different from glibenclamide. The most optimal dose of pandan tikar leaves ethanolic extract is 375 mg/kg bw, with decrease percentage is 56.72%. Conclusions: The ethanolic extract of pandan tikar leaves has antihyperglicemic ability on alloxan-induced diabetic white male rats.

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“…Pada uji flavonoid menggunakan pereaksi magnesium. Pereaksi ini dapat digunakan karena dapat mereduksi terutama senyawa flavonoid dalam suasana asam dengan penambahan HCl (Sopianti, 2018 Kandungan alkaloid didapat pada ekstrak etanol kulit dan biji jeruk namun tidak ditemukan pada sari dari jeruk nipis maupun jeruk sambal. Pada uji Alkaloid ini di dalamnya terdapat atom nitrogen yang bersifat lone pair electron.…”

Section: Skrining Fitokimiaunclassified

Karakterisasi Ekstrak Etanol Buah Citrus amblycarpa (L), Citrus aurantifolia (S.), dan Citrus sinensis (O.)

Fahrurroji

Riza

2020

JFIKI

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Pendahuluan: Buah jeruk manis (Citrus amblycarpa (L)), jeruk nipis (Citrus aurantifolia (S.)), dan jeruk sambal (Citrus sinensis (O.)) memiliki potensi yang sangat besar khususnya kandungan senyawa metabolit sekunder. Tujuan: Pada penelitian ini bertujuan untuk mengetahui karakteristik spesifik dan non spesifik berbagai jenis buah jeruk tersebut pada pelarut etanol 60%. Metode: Ekstrak yang diperoleh ditentukan parameter nonspesifik dan parameter spesifik ekstraknya dari masing-masing ekstrak dari komponen buah jeruk tersebut. Hasil: Berdasarkan hasil pengamatan yang dilakukan Ekstrak etanol 60% kulit dan biji serta air pada buah jeruk tersebut dengan nilai rendemen tertinggi diperoleh pada ekstrak etanol 60% biji jeruk sambal dengan rendemen yang dihasilkan sebesar 25,28%. Seluruh sampel mengandung senyawa fenolik dan flavonoid pada uji tabung. Pada uji KLT fase diam silica gel 60 F254 untuk identifikasi saponin dengan fase gerak kloroform : metanol : air (64 : 50 : 1) dan identifikasi flavonoid menggunakan fase gerak asam asetat glasial : asam formiat : air : etil asetat (11 : 11 : 27 : 100) pada kedua senyawa tersebut menunjukkan reaksi positif pada seluruh sampel uji. Kadar air untuk sampel air jeruk berkisar antara 8,149% - 9,748%. Kesimpulan: Nilai rendemen tertinggi diperoleh pada ekstrak etanol 60% biji jeruk sambal sebesar 25,28%. Pada uji KLT fase diam silica gel 60 F 254 untuk identifikasi saponin dan flavonoid seluruh sampel menunjukkan reaksi positif. Serta kadar abu seluruh sampel pada rentang 2,722 - 5,442%.

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SKRINING FITOKIMIA DAN PROFIL KLT METABOLIT SEKUNDER DARI DAUN RUKU-RUKU (Ocimum tenulflorum L.) DAN DAUN KEMANGI (Ocimum sanctum L)

Cited by 8 publications

References 0 publications

Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta

Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta

Antidiabetic Activity of Ethanolic Extract of Pandan Tikar (Pandanus tectorius) on Alloxan-Induced Diabetic White Male Rats

Karakterisasi Ekstrak Etanol Buah Citrus amblycarpa (L), Citrus aurantifolia (S.), dan Citrus sinensis (O.)

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