Skin Temperature during Cutaneous Wound Healing in an Equine Model of Cutaneous Fibroproliferative Disorder: Kinetics and Anatomic‐Site Differences

Céleste, Christophe; Deschesne, Karine; Riley, Christopher B.; Theoret, Christine L.

doi:10.1111/j.1532-950x.2012.00966.x

Cited by 42 publications

(41 citation statements)

References 38 publications

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“…The skin temperature can also vary depending on the body location and the external conditions, averaging 2–4°C less than the core temperature [10]. Localized inflammation and healing processes can result in elevations (+2–4°C) in the cutaneous wound temperature [11]. These rapid response features which facilitate infection, surface colonization and transfer between hosts imply that the cells react to environmental stimuli by altering their metabolism.…”

Section: Introductionmentioning

confidence: 99%

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

et al. 2014

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Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to survive and capitalize on opportunities to colonize and infect through wound surfaces. It was proposed that S. lugdunensis would have underlying alterations in metabolic homeostasis to provide the necessary levels of adaptive protection. The aims of this project were to examine the impacts of subtle variations in environmental conditions on growth characteristics, cell size and membrane fatty acid composition in S. lugdunensis. Liquid broth cultures of S. lugdunensis were grown under varying combinations of pH (6–8), temperature (35–39°C) and osmotic pressure (0–5% sodium chloride w/w) to reflect potential ranges of conditions encountered during transition from skin surfaces to invasion of wound sites. The cells were harvested at the mid-exponential phase of growth and assessed for antibiotic minimal inhibitory concentration (MIC), generation time, formation of small colony variants, cell size (by scanning electron microscopy) and membrane fatty acid composition. Stress regimes with elevated NaCl concentrations resulted in significantly higher antibiotic resistance (MIC) and three of the combinations with 5% NaCl had increased generation times (P<0.05). It was found that all ten experimental growth regimes, including the control and centroid cultures, yielded significantly different profiles of plasma membrane fatty acid composition (P<0.0001). Alterations in cell size (P<0.01) were also observed under the range of conditions with the most substantial reduction occurring when cells were grown at 39°C, pH 8 (514±52 nm, mean ± Standard Deviation) compared with cells grown under control conditions at 37°C with pH 7 (702±76 nm, P<0.01). It was concluded that S. lugdunensis responded to slight changes in environmental conditions by altering plasma membrane fatty acid composition, growth rates and morphology to achieve optimal adaptations for survival in changing environments.

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Section: Introductionmentioning

confidence: 99%

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

et al. 2014

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“…We used a punch biopsy to create the wounds on the basis of a previous equine model for creating exuberant granulation tissue . Two explanations are possible for our findings in this calf model.…”

Section: Discussionmentioning

confidence: 96%

“…Fifteen minutes after clipping, thermographic imaging of the areas was performed with an infrared thermographic camera (Version 6.3.17178.1001, FLIR Vet T‐420; FLIR Systems, North Billerica, Massachusetts) to obtain baseline images. The camera was held at a constant distance of 1 meter (from the site of interest), and emissivity was set 0.98 on the basis of previous studies . Time frames for still images were 1 second, and the images were corrected for environmental temperature by the camera.…”

Section: Methodsmentioning

confidence: 99%

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Thermographic evaluation of primary closure and second intention healing in dairy calves

et al. 2019

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Objective To evaluate the suitability of infrared thermography in assessing healing of surgically created wounds that are managed by primary closure or second intention in neonatal dairy calves during a 3‐week period. Study design Randomized clinical trial. Animals Six Jersey bull calves. Methods Two skin patches approximately 10 cm2 were shaved on each hind limb of all calves. The dorsal patch was designated the wound creation site, and the ventral patch was the control. The wound creation sites were randomly assigned for either primary closure or healing by second intention. Wounds were created by using an 8‐mm biopsy punch. Thermographic imaging was performed prior to wound creation and at 0 minutes; 15 minutes; 2, 4, 8, 12, and 24 hours; and 2, 3, 4, 7, 10, 14, and 21 days postwounding. Results There were no differences in skin temperature changes observed between wounds that were managed by primary closure or second intention (P = .9934) at any time. Time after wound creation had an effect on the skin temperature (P < .0001), with skin temperature consistently warmer (P < .05) 2, 4, and 8 hours after creation of wounds compared with subsequent times. Conclusion Infrared thermography was unable to detect differences in wound healing by primary closure or second intention in this model. Clinical significance Thermographic monitoring to detect differences in wound healing was not evident in this model. This model might be useful in monitoring temporal changes during early wound repair.

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“…The study of problematic healing of skin wounds on the limbs of horses has provided valuable information on some of the mechanisms underlying this phenomenon. For example, the inflammatory response to wounding in horses is weak [17,22], which may be the result of a state of tissue hypoxia present in limb wounds [24,25] consequent to occlusion of the micro vessels within the wound bed [26,27].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological Study on Wound Distribution Pattern in Horses Presented at Two Veterinary Clinics in South West, Nigeria between 2007- 2010

Kayode¹

2017

JDVAR

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A wound is a breakdown in the protective function of the skin or loss of continuity of epithelium, with or without loss of underlying connective tissues, muscles, nerves, bones following injury to the skin. Wound healing is a survival mechanism and represents an attempt to maintain the normal structure and function. The capacity of a wound to heal depends partly on its depth, overall health, area of the body and nutritional status of the individual. This study presents an epidemiological survey of wounds on different parts of the body in horse presented at state veterinary hospital and Polo club, Ibadan, Nigeria. The data generated were analyzed demographically using descriptive statistics. Out of the total of 84 horses presented for wound cases at State veterinary clinic within this period, wound on the limbs had the highest prevalence of 57% (48), followed by wound on the head and neck with 21% (21), wound on the trunk with 11% (9) while the least prevalent was the wound on the udder with 7% (6). Wound on the leg was the most prevalent condition with 94.2 % (389horses), followed by wound on the head and neck with 2.9% (12), wound on the body with 2.4% (10) while wound on the underbelly was the least prevalent with 0.5% (2) out of the total of 413 horses presented at polo club, Ibadan. Limb wounds are often the most challenging to manage and commonly develop complications, such as the formation of exuberant granulation tissue that will prolong treatment, increase cost and worsen the prognosis.

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Skin Temperature during Cutaneous Wound Healing in an Equine Model of Cutaneous Fibroproliferative Disorder: Kinetics and Anatomic‐Site Differences

Abstract: CWT varied with anatomic location and throughout healing. CWT of wounds developing EGT was significantly less than that of wounds without EGT.

Cited by 42 publications

References 38 publications

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

Thermographic evaluation of primary closure and second intention healing in dairy calves

Epidemiological Study on Wound Distribution Pattern in Horses Presented at Two Veterinary Clinics in South West, Nigeria between 2007- 2010

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