Skin renewal activity of non-thermal plasma through the activation of β-catenin in keratinocytes

Abstract

For recent years, devices that generate non-thermal plasma (NTP) have been introduced into the field of dermatology. Since NTP has demonstrated strong anti-pathogenic activity with safety of use, NTP was first applied to sterilize the skin surface to aid in the healing of various kinds of skin diseases. However, the effect of NTP on skin regeneration has not yet been fully explored. In this study, the effect of NTP on the growth of keratinocytes was tested using the HaCaT human keratinocyte cell line and HRM2 hairless mice. Treatment with NTP allowed confluent keratinocytes to escape from G1 cell cycle arrest and increased the proportion of cells in S and G2 phases. In particular, NTP treatment immediately dispersed E-cadherin-mediated cell-to-cell interactions, resulting in the translocation of β-catenin to the nucleus and leading to the enhanced transcription of target genes including c-MYC and cyclin D1. Moreover, repeated treatment of the mice with NTP also stimulated epidermal expansion by activating β-catenin in the epidermal cells. The symptoms of cellular DNA damage were not detected after NTP treatment. Taken together, these results demonstrate that NTP may be employed as a new type of skin regenerating device.

Plasma Medicine: A Field of Applied Redox Biology

“…These clinical experiences confirm a very early hypothesis in plasma medicine research, that plasma effects on wound healing may be a result of a two-step activity: antiseptics on wound surface in combination with stimulation of tissue regeneration (67) in several in vivo animal experiments (69,(74)(75)(76)(77)(78)(79)(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90) and in human volunteers or patients with reasonably defined wounds (91)(92)(93). It has to be pointed out that these lastmentioned wound healing effects in vivo were demonstrated in acute wounds without any interfering microbial contamination.…”

Human keratinocyte (HaCaT) stimulation and healing effect of the methanol fraction from the decoction from leaf fromSideroxylon obtusifolium(Roem. & Schult.) T.D. Penn on experimental burn wound model

“…A similar result was observed by the methanol fraction of the extract of Centella asiatica (100 μg/ml) that increased the cell migration rate, besides assisting in the remodeling during cicatrization of excisional wounds in rabbits (Azis et al, 2017). Epidermal cell turnover is fundamental for rapid wound healing, in which keratinocytes are stimulated to proliferate by regulating growth factors and intercellular contact (J. H. Choi et al, 2017).…”

Potential Applications of Non-thermal Plasma in Animal Husbandry to Improve Infrastructure

“…They are also essential for a better and fast healing of wounds caused from injuries, ulcers or tumor removal (123,124). A study has demonstrated that NTP can be employed in skin regenerating (125). It was shown that NTP allows keratinocytes to escape from G1 cell cycle arrest, increases the cell proportion in S and G2 phases, causes translocation of beta-catenin to nucleus and induces the transcription of target genes without damaging DNA (125).…”

“…A study has demonstrated that NTP can be employed in skin regenerating (125). It was shown that NTP allows keratinocytes to escape from G1 cell cycle arrest, increases the cell proportion in S and G2 phases, causes translocation of beta-catenin to nucleus and induces the transcription of target genes without damaging DNA (125). Moreover, another study has tested the effects of cold argon plasma on eggs of blow fly (126).…”