Size of the test area does not affect the result of the repeated open application test

Hannuksela, Anna; Niinimäki, Aila; Hannuksela, Matti

doi:10.1111/j.1600-0536.1993.tb03442.x

Cited by 26 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A positive reaction to the test usually appears within 4 days and less frequently between 5 and 7 days (5, 6). The size (at least 1 cm in diameter) of the dosed sites does not grossly affect the results (6). Delayed reactions (post 7 days) have only recently been sought.…”

mentioning

confidence: 99%

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview

2000

View full text Add to dashboard Cite

As one step in defining the clinical relevance of exposure to an allergen identified with patch testing, use tests (provocative use test (PUT), and repeated open application test (ROAT)) have been used. In 1 / 2 of the cases of seemingly reliable patch tests, use tests are negative, suggesting that the patient's biologic threshold of response had not been reached with open application dosing. Dramatic differences exist in regional skin reactivity and percutaneous penetration. Negative results of use tests on normal skin may become positive on diseased skin. To refine this assay further, more controlled observations and analysis of reaction differences between normal and damaged skin, and among regional anatomic sites might be performed. In addition, we require a standardized measurement for the results. Use testing has significant potential in refinement of the evidence-based diagnosis of clinical relevance. However, for general validation, we should fill the deficiencies described above.

show abstract

mentioning

confidence: 99%

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview

2000

View full text Add to dashboard Cite

show abstract

“…Again it would have been interesting to see what would have happened, if there had been a larger difference between the areas. In one study that tested 13 patients with several allergens in a ROAT, no difference was found when the same dose per unit area was applied to 1 and 10 cm 2 , though the reaction appeared later on the small area in some cases (14).…”

Section: Discussionmentioning

confidence: 98%

Dose per unit area - a study of elicitation of nickel allergy

2007

View full text Add to dashboard Cite

show abstract

“…Less common sites were the axillae, dorsal upper arm, neck and scalp. The size of the test area does not seem to affect the result, but the recommendation is to apply the product to a 5 × 5 cm or 10 × 10 cm test site and to apply the product to the same exact area 5 …”

Section: Discussionmentioning

confidence: 99%

What is the optimal duration for a ROAT? The experience of the French Dermatology and Allergology group (DAG)

Amsler

Assier²,

Soria

et al. 2022

Contact Dermatitis

View full text Add to dashboard Cite

show abstract

Size of the test area does not affect the result of the repeated open application test

Cited by 26 publications

References 2 publications

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview

Use tests: ROAT (repeated open application test)/PUT (provocative use test): an overview

Dose per unit area - a study of elicitation of nickel allergy

What is the optimal duration for a ROAT? The experience of the French Dermatology and Allergology group (DAG)

Contact Info

Product

Resources

About