Size Matters: Arginine-Derived Peptides Targeting the PSMA Receptor Can Efficiently Complex but Not Transfect siRNA

Cultrara, Christopher; Shah, Sunil; Antuono, Gina; Heller, Claudia; Ramos, Jorge; Samuni, Uri; Zilberberg, Jenny; Sabatino, David A.

doi:10.1016/j.omtn.2019.10.013

Cited by 9 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All procedures were performed essentially as previously described. 86 In brief, 1 Â 10 7 viable cells were subcutaneously injected into the flanks of mice. Tumor growth was examined every 3 days using a vernier caliper and the mice were sacrificed 4 weeks later.…”

Section: Tumor Inoculation Assay In Nude Micementioning

confidence: 99%

RETRACTED: Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

Chu

Ling

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to be investigated. In this study, we demonstrated that OIP5-AS1 was upregulated in LIHC tissue specimens and its overexpression was associated with the poor survival of patients with LIHC. Furthermore, loss-of function experiments indicated that OIP5-AS1 promoted cell proliferation and inhibited cell apoptosis both in vitro and in vivo. Moreover, binding sites between OIP5-AS1 and hsa-miR-26a-3p as well as between hsa-miR-26a-3p and EPHA2 were confirmed by luciferase assays. Finally, a rescue assay was performed to prove the effect of the OIP5-AS1/hsa-miR-26a-3p/EPHA2 axis on LIHC cell biological behaviors. Based on all of the above findings, our results suggested that OIP5-AS1 promoted LIHC cell proliferation and invasion via regulating the hsa-miR-26a-3p/EPHA2 axis.

show abstract

Section: Tumor Inoculation Assay In Nude Micementioning

confidence: 99%

RETRACTED: Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

Chu

Ling

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…68 However, tumors occur when cancer cells escape the immune system by creating an immunosuppressive environment. [69][70][71][72] Therefore, the focus of recent research has shifted from targeted therapy to immunotherapy, hoping to be used to treat more patients with cancer. This is as a result of immune escape being common for all tumors, and restoring the immune system can help destroy tumors.…”

Section: Next-generation Sequencing and Immunotherapy For Tumorsmentioning

confidence: 99%

Power and Promise of Next-Generation Sequencing in Liquid Biopsies and Cancer Control

Liu

et al. 2020

Cancer Control

View full text Add to dashboard Cite

Traditional methods of cancer treatment are usually based on the morphological and histological diagnosis of tumors, and they are not optimized according to the specific situation. Precision medicine adjusts the existing treatment regimen based on the patient’s genomic information to make it most suitable for patients. Detection of genetic mutations in tumors is the basis of precise cancer medicine. Through the analysis of genetic mutations in patients with cancer, we can tailor the treatment plan for each patient with cancer to maximize the curative effect, minimize damage to healthy tissues, and optimize resources. In recent years, next-generation sequencing technology has developed rapidly and has become the core technology of precise targeted therapy and immunotherapy for cancer. From early cancer screening to treatment guidance for patients with advanced cancer, liquid biopsy is increasingly used in cancer management. This is as a result of the development of better noninvasive, repeatable, sensitive, and accurate tools used in early screening, diagnosis, evaluation, and monitoring of patients. Cell-free DNA, which is a new noninvasive molecular pathological detection method, often carries tumor-specific gene changes. It plays an important role in optimizing treatment and evaluating the efficacy of different treatment options in clinical trials, and it has broad clinical applications.

show abstract

“…However, increasing studies have shown that the incorporation of relatively large, charged fluorophores, bulky hydrophobic drugs and large, complex biologics such as siRNA, plasmid DNA, and proteins can lead to significant changes in the cell uptake mechanism of the CPP (Macchi et al, 2015;Akhunzada et al, 2017). Furthermore, in certain cases cell uptake was abrogated when a CPP targeting the prostate membrane antigen receptor (PSMA) was combined with siRNA due to the formation of large, negatively charged particles according to DLS and TEM (Cultrara et al, 2019). Therefore, extensive structural studies are necessary to fully understand the translocation mechanism of the cargo-bound CPP.…”

Section: Tatmentioning

confidence: 99%

Polyamide Backbone Modified Cell Targeting and Penetrating Peptides in Cancer Detection and Treatment

et al. 2020

Self Cite

View full text Add to dashboard Cite

Cell penetrating and targeting peptides (CPPs and CTPs) encompass an important class of biochemically active peptides owning the capabilities of targeting and translocating within selected cell types. As such, they have been widely used in the delivery of imaging and therapeutic agents for the diagnosis and treatment of various diseases, especially in cancer. Despite their potential utility, first generation CTPs and CPPs based on the native peptide sequences are limited by poor biological and pharmacological properties, thereby restricting their efficacy. Therefore, medicinal chemistry approaches have been designed and developed to construct related peptidomimetics. Of specific interest herein, are the design applications which modify the polyamide backbone of lead CTPs and CPPs. These modifications aim to improve the biochemical characteristics of the native peptide sequence in order to enhance its diagnostic and therapeutic capabilities. This review will focus on a selected set of cell penetrating and targeting peptides and their related peptidomimetics whose polyamide backbone has been modified in order to improve their applications in cancer detection and treatment.

show abstract

Size Matters: Arginine-Derived Peptides Targeting the PSMA Receptor Can Efficiently Complex but Not Transfect siRNA

Cited by 9 publications

References 21 publications

RETRACTED: Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

RETRACTED: Long Noncoding RNA OIP5-AS1 Promotes the Progression of Liver Hepatocellular Carcinoma via Regulating the hsa-miR-26a-3p/EPHA2 Axis

Power and Promise of Next-Generation Sequencing in Liquid Biopsies and Cancer Control

Polyamide Backbone Modified Cell Targeting and Penetrating Peptides in Cancer Detection and Treatment

Contact Info

Product

Resources

About