Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21

Fitriani, Fajariana; Soetojo, Adioro; Subiwahjudi, Agus; Yuanita, Tamara

doi:10.20473/cdj.v9i1.2019.54-65

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…This value meant it was non-toxic as denture cleansing agent because the toxicity threshold value was >70%. 24 According to Widayat et al, 25 50% concentration of mangosteen peel extract was able to reduce the number of Streptococcus mutans colonies with an average number of bacterial colonies of 0.67. Mangosteen peel extract with a concentration of 50% was non-toxic on BHK-21 fibroblast cell due to its content i.e.…”

Section: Discussionmentioning

confidence: 99%

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Tertya,

Kristiana,

Naini

2024

Padjadjaran J Dent

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Introduction: Mangosteen peel extract can be used as a denture cleaning agent to effectively inhibit the growth of C. albicans because mangosteen peel contains various compounds such as mangostin, tannins, flavonoids, saponins. Denture cleaning materials that will be used in the dental field must meet biocompatibility requirements, one of which is a toxicity test. The aim of this study was to analyze the toxicity of mangosteen peel extract as a cleaner of heat cured acrylic resin denture on BHK-21 as cell culture of fibroblast cells. Methods: Toxicity test used the MTT assay method. Acrylic resin samples had a diameter of 3 mm and a thickness of 2 mm. The acrylic resin was soaked for 4 days, equivalent to soaking 15 minutes per day for 1 year in mangosteen peel extract with concentrations of 50, 60, 70%, and sodium hypochlorite. Analyzed data using normality and homogeneity test results showed (p<0.05). Kruskal Wallis test results (0.000<0.05) and Mann Whitney (0.000<0.05). Results: The greatest cell viability value was shown in mangosteen peel extract with a concentration of 50%, namely 97.16939%. Based on research data, it can be said that the lower the concentration of mangosteen peel extract, the lower the level of toxicity of a denture cleanser agent. Mangosteen peel extract in concentrations of 60, 70%, and sodium hypochlorite are toxic when used as a denture cleanser. Conclusion: Mangosteen peel extract with a concentration of 50% is not toxic to BHK-21 fibroblast cells as cell culture of and as a cleaner of heat cured acrylic resin denture on.Keywordheat-cured acrylic resin, mangosteen peel extract, MTT assay.

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Section: Discussionmentioning

confidence: 99%

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Tertya,

Kristiana,

Naini

2024

Padjadjaran J Dent

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“…ROS are produced in normal mitochondrial respiration and will be degraded by the immune system. 1,18 Excessive free radicals known as oxidative stress are usually involved in cell damage. The presence of free radicals causes lipid peroxidation reactions in plasma membranes and organelles.…”

Section: Discussionmentioning

confidence: 99%

“…Initially, there will be disturbances in cell permeability which causes cells to become necrotic. This occurs due to the accumulation of compounds and the breakdown of fat due to the bond between cell lipoproteins and polar compounds in tannins 18. The normal functioning of enzymes and protein-based mechanisms in cells operate over a very narrow pH range of around 7.0.…”

mentioning

confidence: 99%

Cytotoxicity test of apple cider vinegar as a root canal irrigant against fibroblast cells

Lesmana¹,

Djuanda²,

Sugiaman³

2022

Odonto : Dental Journal

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Background: Apple cider vinegar potentially can be used as an alternative to irrigation solutions because of its antibacterial compounds that can inhibit Enterococcus faecalis, a pioneer bacteria that cause root canal treatment failure. One of the ideal irrigation solution requirements is that it isn't toxic to oral cavity tissues, so it's necessary to run a cytotoxicity test on apple vinegar solution. Cytotoxicity test is the initial part of the evaluation of a dental material before it can be used by humans. Cytotoxicity test was performed on fibroblast cells because the irrigation solution can contact with fibroblast, which are the main cells in the periodontal ligament around the apical. The purpose of this study was to analyze the in vitro cytotoxicity effect of ACV on fibroblast cells. Method: Apple vinegar with concentrations of 0.31%, 0.63%, 1.25%, 2.5%, and 5% was tested using the MTS assay method. Result: The results showed that there was a cytotoxicity effect of apple vinegar solution as a root canal irrigation agent against fibroblasts cell. Apple cider vinegar with concentrations of 1.25%, 2.5%, and 5% are potentially toxic because the percentage of cell viability is less than 70%. Conclusion:There is a cytotoxicity effect of apple cider vinegar solution as a root canal irrigant on fibroblast cells.

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“…This was attributed to the toxic effect of the alkaloids and flavonoids at high concentrations. 28 However, alkaloids do not significantly cause toxic effects, 29 whereas, flavonoids at relatively high concentrations trigger ROS formation, causing toxic effects on cells. 30 However, the flavonoids detected at a high concentration in Acacia rugata L had no effect on rat kidney.…”

Section: In Vitro Cytotoxicity Of Fdle Cell Viability Was Assessed Ba...mentioning

confidence: 99%

In Vitro and In Vivo Nephrotoxicity Evaluation of the Methanol Extract of Ficus deltoidea Jack Leaf

2024

TJNPR

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Ficus deltoidea Jack leaves extract (FDLE) is rich in phytochemicals like alkaloids, phenolics, flavonoids, and steroids. These phytochemicals possess an excellent potential for medicinal applications. This study aimed to assess the kidney toxicity of FDLE through in vitro and in vivo methods. FDLE was obtained by maceration in methanol at room temperature for 24 h. The in vitro toxicity of FDLE at 10 -100 µg/mL was evaluated in Baby Hamster Kidney (BHK)-21fibroblast cells using the MTT assay method. The in vivo toxicity was assessed by histopathological examination of the kidneys of Wistar rats following oral acute administration of FDLE at 1.250 mg/kg, 2.500 mg/kg, and 3.750 mg/kg twice daily for 14 days. In vitro toxicity tests showed no toxic effect of FDLE on BHK-21 fibroblast cells. The percentage cell viability of BHK-21 fibroblast cells was higher than 60% at all the concentration of FDLE tested, and the IC50 value was 71.63 µg/mL. The histopathological analysis revealed no significant difference in the haemarrage and necrosis observed in the tissues of the kidneys of rats in the FDLE treated groups compared to the control group. Therefore, it could be concluded that FDLE demonstrated no toxic effects in vitro and in vivo, and may be relatively safe when administered to humans.

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Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21

Cited by 5 publications

References 17 publications

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory

Cytotoxicity test of apple cider vinegar as a root canal irrigant against fibroblast cells

In Vitro and In Vivo Nephrotoxicity Evaluation of the Methanol Extract of Ficus deltoidea Jack Leaf

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