Site-directed immobilization of antibody onto solid surfaces for the construction of immunochip

Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

“…As shown in Fig. 6, we achieved typical curves of resonant frequency change as a function of the myoglobin antigen concentration and these curves are similar to that of the ELISA obtained by Cho et al [23]. Considering the variation of sensing result, the estimated limit of detection of site-directed antibody is around 1 ng/ml, while that of random antibody is 10 ng/ml.…”

“…This site-directed immobilization leads to high activity of sensing layer. Although we showed that the site-directed antibody enhanced the sensitivity using optical method [21][22][23], no report was published about the effect of the site-directed antibody on the microcantilever sensor. We adopted the site-directed immobilization to the surface of cantilever and investigated its effect on the electrical sensing of resonance frequency.…”

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

“…These networks are characterized by a large surface area to volume ratio and can contain over 90% water. The increased surface area enables ideal conditions for antibody immobilization, which is achieved through the binding of biotinylated antibodies to avidin protein, reducing the risk of steric effects [32][33][34]. Previously reported methods for the synthesis of these networked hydrogel particles have employed a platform called stop flow lithography (SFL) [27], in which UV light is used to polymerize the PEG hydrogels of various 2D extruded shapes inside a microfluidic channel.…”

Selective immobilization of proteins on gold dot arrays and characterization using chemical force microscopy

“…To increase the activity of antibodies, chemical activation and fragmentation of antibodies are very useful methods for controlling orientation of antibodies on a surface [25][26][27][28].…”