Sister chromatid exchange in selected horse breeds (&amp;lt;i&amp;gt;Equus caballus&amp;lt;/i&amp;gt;)

Wójcik, Ewa; Smalec, Elżbieta; Danielewicz, A.

doi:10.5194/aab-54-107-2011

Cited by 11 publications

(21 citation statements)

References 30 publications

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“…Few publications contain reports about statistically significant differences in SCE incidence between males and females (Margolin and Shelby 1985;Husum et al 2008;Szeleszczuk et al 2014). Comprehensive studies of farm animals have not confirmed the effect of the sex on SCE frequency (Iannuzzi et al 1991b;Di Meo et al 1993, 2000Ciotola et al 2005;Peretti et al 2006;Wo´jcik et al 2011Wo´jcik et al , 2013Wo´jcik et al , 2014Wo´jcik and Smalec 2012a). The present authors also did not observe significant differences in the mean number of SCEs between chinchillas of different sexes.…”

Section: Discussioncontrasting

confidence: 80%

“…Age significantly affects the incidence of SCE. Older animals develop more SCEs per cell compared with younger animals (Sinha et al 1985;Lazutka et al 1994;Peretti et al 2006;Husum et al 2008;Wnuk et al 2011;Wo´jcik et al 2011), and differences in the numbers of SCEs between old and young chinchillas were also observed in the present study. We observed a highly interesting interrelationship.…”

Section: Discussionsupporting

confidence: 78%

“…(1 was an optional dose (Nicolae et al 2009;Murali and Panneerselvam 2011;Wo´jcik et al 2011;Wo´jcik and Smalec 2012a, b). The lowest BrdU levels, 0.1, 0.25, 0.5, 1.0, and 5.0 mg BrdU mL…”

Section: Discussionmentioning

confidence: 99%

“…The mean incidence of SCE in the chinchillas in the present study was identified at 4.34. Different animal species have different spontaneous SCE/ cell means: in the Chinese hamster from 2.3 (Kato 1974) to 6.2 (Wo´jcik et al 2003), in cattle 2.48 (Di Berardino et al 1995), in goats from 2.7 (Wo´jcik and Smalec 2012a) to 6.64 (Di Meo et al 1993), in horses from 3.6 to 6.6 (Wo´jcik et al 2011), in the domestic cat 3.4 (Szeleszczuk et al 2014). The discrepancies between results for the same species may result from the use of different breeds by different researchers.…”

Section: Discussionmentioning

confidence: 99%

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Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

et al. 2015

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Kuchta-Gl adysz, M., Wo´jcik, E., Szeleszczuk, O., Niedbal a, P. and Tyblewska, K. 2015. Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera). Can. J. Anim. Sci. 95: 543Á550. The sister chromatid exchange (SCE) test is a cytogenetic tool with applications as a short-term screen. It is used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping to predict possible genetic consequences in animal cells and assess animal resistance. The mitotic chromosomes were obtained from an in vitro culture of peripheral blood lymphocytes with added bromodeoxyuridine (BrdU), at five different concentrations: 0.25, 0.5, 1.0, 2.5, and 5.0 mg mL (1 . The chromosomes were stained according to the FPG method. Our analyses revealed the spontaneous SCE level in the chinchilla at the concentration of 0.5 mg mL (1 . Higher concentrations of this substance have a genotoxic effect and cause damage to the DNA structure of the chromosomes by inducing additional SCEs in the chromosomes of this species. The mean SCE/cell incidence in the chinchilla population was 4.3491.28. We investigated the effects of age on the incidence of SCE and found it significantly affected this phenomenon in both sexes.

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Section: Discussioncontrasting

confidence: 80%

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

et al. 2015

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“…For a more complete assessment of somatic mutagenesis of mesenchymal stem cells, a micronucleus test was performed. The formation of cells with micronuclei is the result of chromosomal ruptures or defects in the mitotic spindle, which is consistent with the manifestation of aneuploidy (15,18,19,(22)(23). The normal frequency of cells with micronuclei in mammals is 1.6‰-5.6‰ (8).…”

Section: Discussionsupporting

confidence: 53%

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Mazurkevych

Malyuk

Bezdieniezhnykh

et al. 2016

Journal of Veterinary Research

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Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture. Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study. Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells. Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.

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Identification of chromosome instability in Japanese quail (Coturnix japonica)

Wójcik

Andraszek

Smalec

et al. 2014

British Poultry Science

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1. A study of the incidence of chromosome instability in the Japanese quail as assessed by sister chromatid exchange (SCE) and fragile site identification in chromosomes was conducted in two parent breeds and their F1 and F2 generations. 2. The mean incidence of SCEs was 6.02 ± 0.45 and the frequency of fragile sites was 1.17 ± 0.79. 3. There were moderately negative correlations of 0.51-0.64 between chromosome instability and fertility in the F1 and 0.10-0.23 in the F2. The hatch of fertilised eggs was negatively correlated with the number of SCE in male (0.31) and female (0.33) F1 and was lower in P (0.18 and 0.19, respectively), whereas the correlations were similar for the number of fragile sites in both generations (0.51-0.62).

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Sister chromatid exchange in selected horse breeds (<i>Equus caballus</i>)

Cited by 11 publications

References 30 publications

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Identification of chromosome instability in Japanese quail (Coturnix japonica)

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