Identification of chromosome instability in Japanese quail (<i>Coturnix japonica</i>)

Wójcik, Ewa; Andraszek, Katarzyna; Smalec, Elżbieta; Knaga, Sebastian; Witkowski, A.

doi:10.1080/00071668.2014.929637

Cited by 6 publications

(10 citation statements)

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“…Few publications contain reports about statistically significant differences in SCE incidence between males and females (Margolin and Shelby 1985;Husum et al 2008;Szeleszczuk et al 2014). Comprehensive studies of farm animals have not confirmed the effect of the sex on SCE frequency (Iannuzzi et al 1991b;Di Meo et al 1993, 2000Ciotola et al 2005;Peretti et al 2006;Wo´jcik et al 2011Wo´jcik et al , 2013Wo´jcik et al , 2014Wo´jcik and Smalec 2012a). The present authors also did not observe significant differences in the mean number of SCEs between chinchillas of different sexes.…”

Section: Discussionsupporting

confidence: 44%

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Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

et al. 2015

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Kuchta-Gl adysz, M., Wo´jcik, E., Szeleszczuk, O., Niedbal a, P. and Tyblewska, K. 2015. Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera). Can. J. Anim. Sci. 95: 543Á550. The sister chromatid exchange (SCE) test is a cytogenetic tool with applications as a short-term screen. It is used to assess the influence of physical and chemical factors with potential mutagenic and genotoxic properties on the animal organism. The test results make it possible to eliminate mutagens, as well as helping to predict possible genetic consequences in animal cells and assess animal resistance. The mitotic chromosomes were obtained from an in vitro culture of peripheral blood lymphocytes with added bromodeoxyuridine (BrdU), at five different concentrations: 0.25, 0.5, 1.0, 2.5, and 5.0 mg mL (1 . The chromosomes were stained according to the FPG method. Our analyses revealed the spontaneous SCE level in the chinchilla at the concentration of 0.5 mg mL (1 . Higher concentrations of this substance have a genotoxic effect and cause damage to the DNA structure of the chromosomes by inducing additional SCEs in the chromosomes of this species. The mean SCE/cell incidence in the chinchilla population was 4.3491.28. We investigated the effects of age on the incidence of SCE and found it significantly affected this phenomenon in both sexes.

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Section: Discussionsupporting

confidence: 44%

“…However, to a certain extent, they correspond with normal cellular processes (Shaffer 1977;Painter 1980;Wo´jcik et al 2004). Approximately 10 SCEs spontaneously occur during a normal cell cycle in man.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

et al. 2015

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“…Different frequencies of FS have been observed in buffalo by Albarella et al [66], Di Meo et al [67], Genualdo et al [68] and Kumar et al [69]. Different FS values have also been observed in various breeds of pigs [64,70,71], sheep [64,72–74], chickens, and quails [32,75]. The results obtained by these researchers indicate that breed has a major influence on the incidence of FS.…”

Section: Discussionmentioning

confidence: 99%

“…The results obtained by these researchers indicate that breed has a major influence on the incidence of FS. Wójcik et al [75], in a study on quail, evaluated pure breeds (Japanese quail and Pharaoh Coturnix) as well as crosses of these breeds in the F1 and F2 generations. The authors observed the most chromosome damage in the parent generation, slightly less in the F1 generation (crossbreds) and the least in the F2 generation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genome stability in various breeds of cattle

Wójcik

Szostek

2019

PLoS ONE

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Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).

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“…Checkpoints in the cell cycle may monitor these regions and block entry into mitosis before their replication is complete (Durkin & Glover, 2007). Therefore, fragile sites are found in all animal species: pigs, cattle, sheep, chickens, geese, and quail (Albarella et al., 2009; Ali, Abdullah, Babar, Javed, & Nadeem, 2008; Gordon et al., 2007; Rønne, 1995; Wójcik, Andraszek, Smalec, Knaga, & Witkowski, 2014; Wójcik & Smalec, 2012; Wójcik & Szostek, 2019; Wójcik et al., 2018). Unfortunately, there are no publications concerning fragile sites identified in coypus.…”

Section: Discussionmentioning

confidence: 99%

Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu)

Kuchta-Gładysz

Wójcik

Słonina

et al. 2020

Animal Science Journal

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Coypus (Myocastor coypu) are fur-bearing herbivores native to South America (Gosling & Berker, 1991). In European countries, coypus have been bred since the 1920s (Carter & Leonard, 2002). In Poland, coypu breeding experienced a decline in the late 1990s and the number of animals began to fall quickly. Although coypu pelts and meat are valuable, there was no demand for these products (Kowalska & Niedbała, 2014). Furthermore, coypu breeding was adversely affected by environmental movement campaigns and rising farm maintenance costs. For this reason, coypus were included in a genetic resources conservation program for native breeds, which is aimed to preserve and increase population size while maintaining genetic variation in the coypu (http://www.bioro znoro dnosc.izoo.krakow. pl). The loss of interest in the breeding of this species did not prevent researchers from conducting numerous studies, including the determination of genetic diversity in the populations and karyotype characterization using available cytogenetic techniques (Kaplanova et al., 2012). In the coypu karyotype (2n = 42), all the autosomes are bi-armed: 32 are metacentric and 8 are submetacentric. The X sex chromosome is metacentric and the Y is the smallest chromosome of

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Identification of chromosome instability in Japanese quail (Coturnix japonica)

Cited by 6 publications

References 50 publications

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

Spontaneous sister chromatid exchange in mitotic chromosomes of the chinchilla (Chinchilla lanigera)

Assessment of genome stability in various breeds of cattle

Determination of cytogenetic markers for biological monitoring in coypu (Myocastor coypu)

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