SIRT1: A Potential Therapeutic Target in Autoimmune Diseases

Shen, Pan; Deng, Xuan; Chen, Zhe; Ba, Xin; Qin, Kai; Huang, Ying; Huang, Yao; Li, Tingting; Yan, Jiahui; Tu, Shenghao

doi:10.3389/fimmu.2021.779177

Cited by 81 publications

(45 citation statements)

References 112 publications

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“…SIRT1, the most outstanding and most extensively investigated member among Sirtuins, is known as a nuclear protein that regulates biological processes like inflammation, oxidative stress, mitochondrial function, immune response, cell differentiation, proliferation, and metabolism [ 57 ]. SIRT1 signaling pathway activation can suppress inflammatory cytokines and cell apoptosis to modulate brain damage incurred by ischemic stroke [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

et al. 2022

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Background. Chlorogenic acid (CGA) is a polyphenolic compound with antioxidant and anti-inflammatory properties. CGA has been shown to improve neuroinflammation. This study is aimed at elucidating the exact mechanism by which CGA reduces neuroinflammation. Methods. Oxygen and glucose deprivation (OGD) was utilized to treat BV2 microglia and HT-22 hippocampal neurons to engineer an in vitro model of hypoxic ischemia reperfusion. The levels of inflammatory factors (IL-1β, IL-6, TNF-α, IL-4, and IL-10) and oxidative stress factors (MDA, SOD, and GSH-PX) in microglia were determined by ELISA kits. The neuron proliferation was assessed by CCK-8 assay, and LDH kit was used to determine LDH release in neurons. The fluorescent dye DCF-DA was employed to measure ROS levels in neurons. Correlation of MIR497HG, miR-29b-3p, and SIRT1/NF-κB in neurons and microglia was determined by qRT-PCR. Expressions of inflammatory proteins (COX2, iNOS), oxidative stress pathways (Nrf2, HO-1), and apoptosis-related proteins (Bcl-2, Bax, caspase3, caspase8, and caspase9) in microglia or neurons were determined by western blot. The interactions between MIR497HG and miR-29b-3p, as well as between miR-29b-3p and SIRT1, were determined by dual luciferase assay and RIP assay. Results. CGA attenuated OGD-mediated inflammation and oxidative stress in microglia and inhibited microglia-mediated neuronal apoptosis. CGA increased the levels of MIR497HG and SIRT1 and suppressed the levels of miR-29b-3p in BV2 and HT-22 cells. MIR497HG knockdown, miR-29b-3p upregulation, and SIRT1 inhibition inhibited CGA-mediated anti-inflammatory and neuronal protective functions. There is a targeting correlation between MIR497HG, miR-29b-3p, and Sirt1. MIR497HG sponges miR-29b-3p to regulate SIRT1 expression in an indirect manner. Conclusion. CGA upregulates MIR497HG to curb miR-29b-3p expression, hence initiating the SIRT1/NF-κB signaling pathway and repressing OGD-elicited inflammation, oxidative stress, and neuron apoptosis.

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Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

et al. 2022

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“…Previous studies have proposed that NAM plays a beneficial role mainly by increasing the levels of NAD + [ 58 ]. The increase of NAD + level can reduce the production of mitochondrial ROS by regulating the activity of NAD + consuming enzyme sirtuin1 [ 59 ]. As NAD+ and sirtuin1 activity levels decline with age [ 8 ], targeted increase of NAD+ levels has become a potential therapeutic approach to ameliorate aging-related diseases and prolong human lifespan.…”

Section: Discussionmentioning

confidence: 99%

Effects of nicotinamide on follicular development and the quality of oocytes

Yang

Feng

et al. 2022

Reprod Biol Endocrinol

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Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress.

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“…SIRT1, a nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase, is able to regulate inflammation, oxidative stress, mitochondrial function, immune response, cell differentiation, proliferation, and metabolism, and its dysfunction may participate in the development of autoimmune diseases ( 231 ). In recent years, there are more and more studies on the regulation of energy metabolism and immune function of SIRT1 in RA ( 232 ).…”

Section: Mechanism Of Action Against Ramentioning

confidence: 99%

Natural medicines of targeted rheumatoid arthritis and its action mechanism

Wang

Qian

et al. 2022

Front. Immunol.

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Rheumatoid arthritis (RA) is an autoimmune disease involving joints, with clinical manifestations of joint inflammation, bone damage and cartilage destruction, joint dysfunction and deformity, and extra-articular organ damage. As an important source of new drug molecules, natural medicines have many advantages, such as a wide range of biological effects and small toxic and side effects. They have become a hot spot for the vast number of researchers to study various diseases and develop therapeutic drugs. In recent years, the research of natural medicines in the treatment of RA has made remarkable achievements. These natural medicines mainly include flavonoids, polyphenols, alkaloids, glycosides and terpenes. Among them, resveratrol, icariin, epigallocatechin-3-gallate, ginsenoside, sinomenine, paeoniflorin, triptolide and paeoniflorin are star natural medicines for the treatment of RA. Its mechanism of treating RA mainly involves these aspects: anti-inflammation, anti-oxidation, immune regulation, pro-apoptosis, inhibition of angiogenesis, inhibition of osteoclastogenesis, inhibition of fibroblast-like synovial cell proliferation, migration and invasion. This review summarizes natural medicines with potential therapeutic effects on RA and briefly discusses their mechanisms of action against RA.

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SIRT1: A Potential Therapeutic Target in Autoimmune Diseases

Cited by 81 publications

References 112 publications

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

Chlorogenic Acid Prevents Microglia-Induced Neuronal Apoptosis and Oxidative Stress under Hypoxia-Ischemia Environment by Regulating the MIR497HG/miR-29b-3p/SIRT1 Axis

Effects of nicotinamide on follicular development and the quality of oocytes

Natural medicines of targeted rheumatoid arthritis and its action mechanism

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