Effects of nicotinamide on follicular development and the quality of oocytes

Abstract

Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress.

Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes

“…Therefore, how to improve the oocyte quality of infertile women with diabetes to achieve comparative developmental competence with healthy females is an important research and clinical topic. The IVM culture medium plays critical role in achieving higher IVM rate of oocytes [ 14 , 34 ]. Several drugs have been proved to have the ability to improve oocyte quality when supplemented in the culture medium.…”

“…Therefore, overcoming the problems correlated with IVM by optimizing the IVM system is of significant importance. Previous studies have shown that supplementation of antioxidants during the IVM process could improve the oocyte quality and reduce the damage caused by oxidative stress [ 5 , 14 , 15 ]. In line with this finding, we aimed to explore novel strategies to improve the maturation rate and quality of IVM oocytes of patients suffering from diabetes, thereby increase the assisted reproductive efficiency and ultimately improve the reproductive outcomes of these patients.…”

The walnut-derived peptide TW-7 improves mouse parthenogenetic embryo development of vitrified MII oocytes potentially by promoting histone lactylation

“…TW-7 (25, 50, and 100 μmol/L), oxamate (5 mmol/L, a glycolysis inhibitor that reduces intracellular lactate levels [ 47 , 48 ], HY-W0130032A, MedChemExpress, Monmouth Junction, NJ, USA), and lactate (5 mmol/L, a histone lactation agonist [ 28 ], HY-B2227B, MedChemExpress) were supplemented in the warming, recovery, PA and IVC medium. Hydrogen peroxide (H 2 O 2 , 100 μmol/L) was added to the IVC medium as an oxidative stress inducer for 1 h [ 49 ].…”

Supplementation with NAD+ and its precursors: A rescue of female reproductive diseases

“…Maintenance of follicular NAD + levels can be achieved by supplementation with NAD + precursors. Supplementation with NAD + precursors (such as NMN, NR, NA, and NAM) protects the first meiosis of aged oocytes and reduces the incidence of defects in spindle components such as spindle and chromosome and aneuploidy while improving the number of pregnancies and live births in aged mouse ( Table 1 ) [ 32 , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] ]. Most of the mechanisms that NAD + precursors protecting ovarian preservation are through improving mitochondrial function, decreasing ROS levels, reducing oxidative damage, inhibiting DNA damage, and apoptosis ( Fig.…”