Single step cell lysis/PCR detection of Escherichia coli in an independently controllable silicon microreactor

Ke, Cathy; Kelleher, Ann; Berney, Helen; Sheehan, Michelle M.; Mathewson, A.

doi:10.1016/j.snb.2006.03.019

Cited by 47 publications

(38 citation statements)

References 16 publications

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“…It is worth noting that a same rescaled standard deviation requires a resistor area 3.36 times larger in a nonoptimized case; otherwise, a similar resistor size leads to a standard deviation ratio of roughly 8, and this improvement is significant. Figure 8 exhibits temperature rise in the transient state and reveals that thermal accommodation time does not exceed 2.2 s. It should also be pointed out that this short transient state may prove very advantageous for applications requiring rapid temperature changes (e.g., PCR applications (Liao et al 2005;Neuzil et al 2006;Ke et al 2006)). …”

Section: Discussion On System Performancementioning

confidence: 95%

“…We are proposing a method to control heat within a microfluidic cavity (of the microreactor type) using a heating resistance whose size is comparable to that of the cavity. Most of the reported results in the literature involve either whole system heating (Ke et al 2006;Goulpeau et al 2005) or a heating zone of size much larger than the interest zone (Liao et al 2005). Approaches to accurate temperature control within microfluidic systems have been proposed.…”

Section: Introductionmentioning

confidence: 99%

“…Finally, the miniaturization of biological protocols offers valuable insight, especially as regards portability, the small quantity of reagent needed, a decrease in the number of contaminations (Goulpeau et al 2005), and fast heat transfers. These advantages have been utilized over the past several years for purposes of amplifying nucleic acids on chips (polymerase chain reaction, PCR) (Ke et al 2006;Liao et al 2005). Mahjoob et al (2008) have proposed the insertion of a porous matrix to increase heating/cooling rates due a very large surface area for a given volume, which is a key parameter in heat transfer processes.…”

Section: Introductionmentioning

confidence: 99%

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Integration of a uniform and rapid heating source into microfluidic systems

Selva

Mary

Jullien

2009

Microfluid Nanofluid

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The purpose of this study has been to demonstrate the possibilities of uniform heating of a cavity, with great accuracy, by means of an integrated resistor built with the same dimensions as the cavity, i.e., with a high level of integration. For application purposes, a compact resistor allows increasing the number of cavities in which temperature can be independently controlled on the same substrate, which can prove critical for high-throughput screening applications. Potential applications lie in the field of biology or chemistry. In order to achieve the desired result, an optimization procedure was performed on the shape of the resistor. The heater size reduction enables a high level of integration with a reduced heating source surface area. Resistor shape has been optimized to reduce the influence of boundary effects, using improvements introduced in genetic algorithms. An experimental validation of the temperature profile inside the cavity has been carried out using a dye whose fluorescence depends on temperature, i.e., Rhodamine B, it will be shown herein that the optimized resistor allows for temperature cycling, e.g., for PCR applications.

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Section: Discussion On System Performancementioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Integration of a uniform and rapid heating source into microfluidic systems

Selva

Mary

Jullien

2009

Microfluid Nanofluid

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“…Recently, Ke et al [52] reported a silicon substrate microchip eliminating thermal crosstalk with neighboring reaction cavities. The lysis of E. coli was performed during a heating step within 2 min at 957C immediately prior to the start of PCR thermocycling.…”

Section: Thermal Lysismentioning

confidence: 99%

Chemical cytometry on microfluidic chips

Yan

Zhang

2008

Electrophoresis

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Chemical cytometry, referring to the analysis of the chemical contents in individual cells, has been in intensive study since Kennedy's first work that was published in Science. The early researches relied on fine-tip capillaries to capture the cells and do the analyses, which were lab- and time-intensive and required high skills of operation. The emergence of microfluidics has greatly spurred this research field and a great number of research papers have been published in the last decades. Highly integrated microfluidic chips have been developed to capture multiple single cells, lyse them, perform chemical reactions in enclosed microchambers, separate contents by CE and detect chemical species in individual cells. This review focuses on the development of relevant components and their integration for on-chip chemical cytometry.

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“…A device that allows fast heating/cooling rates, and therefore, giving efficient thermocycling suitable for DNA amplification has been developed (Ke et al 2004). A novel device involving the cell lysis and DNA amplification performed in a single step have been proposed by Ke et al (2007). A simple and rapid protocol for the preparation of total genomic DNA from the yeast and mould was proposed by Borman et al (2006Borman et al ( , 2008.…”

Section: Introductionmentioning

confidence: 99%