Chemical cytometry on microfluidic chips

Yan, Hui; Zhang, Boyang; Wu, Hongkai

doi:10.1002/elps.200700561

Cited by 23 publications

(13 citation statements)

References 145 publications

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“…Copyright 2009 American Chemical Society) the literature, there are not many examples of platforms integrating various sample preparation steps. Many of the problems related to single-cell sample preparation, offering advantages in terms of integration of multiple steps as well as miniaturization, can be solved by employing microfluidics [102][103][104][105][106]. An example of this direction is the study carried out by Wu et al [40]: the authors used a microfluidic chip to address different sample preparation steps, including cell handling, lysis, chemical derivatization, separation, and detection of several amino acids (Fig.…”

Section: Sample Preparation: a Key Challengementioning

confidence: 99%

Analytical techniques for single-cell metabolomics: state of the art and trends

2010

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Single-cell metabolomics is an emerging field that addresses fundamental biological questions and allows one to observe metabolic phenomena in heterogeneous populations of single cells. In this review, we assess the suitability of different detection techniques and present considerations on sample preparation for single-cell metabolomics. Although targeted analysis of single cells can readily be conducted using fluorescent probes and optical instruments (microscopes, fluorescence detectors), a comprehensive metabolomic approach requires a powerful label-free method, such as mass spectrometry (MS). Mass-spectrometric techniques applied to study small molecules in single cells include electrospray MS, matrix-assisted laser desorption/ionization MS, and secondary ion MS. Sample preparation is an important aspect to be taken into account during further development of methods for single-cell metabolomics.

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Section: Sample Preparation: a Key Challengementioning

confidence: 99%

Analytical techniques for single-cell metabolomics: state of the art and trends

2010

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“…To date, several methods have been demonstrated to lyse cells in microfluidic devices. 1,2 Chemical lysis uses lytic agents such as sodium dodecyl sulfate to dissolve the cell membrane or react with the membrane lipids. [3][4][5] Mechanical lysis uses nanoscale filtrations, 6 spherical particles, 7 or microscale sonication 8 to break down cells with shear and/or frictional forces.…”

Section: Introductionmentioning

confidence: 99%

Integrated electrical concentration and lysis of cells in a microfluidic chip

et al. 2010

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Lysing cells is an important step in the analysis of intracellular contents. Concentrating cells is often required in order to acquire adequate cells for lysis. This work presents an integrated concentration and lysis of mammalian cells in a constriction microchannel using dc-biased ac electric fields. By adjusting the dc component, the electrokinetic cell motion can be precisely controlled, leading to an easy switch between concentration and lysis of red blood cells in the channel constriction. These two operations are also used in conjunction to demonstrate a continuous concentration and separation of leukemia cells from red blood cells in the same microchannel. The observed cell behaviors agree reasonably with the simulation results.

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“…There have been extensive applications of microfluidics for miniaturized cell culture, isolation, trapping, concentration, and chemical cellular monitoring after exposure to a stimulus. Other recent review articles have summarized current developments of microfluidic chips for cell sorting applications, single cell analysis, cell culture, and cell biological assays [16, 60–63]. For the scope of this review, attention was given to those microfluidic applications of cellular analysis that measured general metabolic activity or that monitored targeted metabolic products.…”

Section: Microfluidics For Cellular Analysismentioning

confidence: 99%

Review: Microfluidic applications in metabolomics and metabolic profiling

Kraly

Holcomb

Guan

et al. 2009

Analytica Chimica Acta

118

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Metabolomics is an emerging area of research focused on measuring small molecules in biological samples. There are a number of different types of metabolomics, ranging from global profiling of all metabolites in a single sample to measurement of a selected group of analytes. Microfluidics and related technologies have been used in this research area with good success. The aim of this review article is to summarize the use of microfluidics in metabolomics. Direct application of microfluidics to the determination of small molecules is covered first. Next, important sample preparation methods developed for microfluidics and applicable to metabolomics are covered. Finally, a summary of metabolomic work as it relates to analysis of cellular events using microfluidics is covered. KeywordsMicrofluidics; metabolomics; metabolic profiling; review I INTRODUCTIONThe goal of this review is to summarize the use of microfluidics as a novel tool set for metabolomics, metabolic profiling, and other metabolite related biological studies. To the knowledge of the authors this is the first such review generated, and thus we will include articles dating back as far as 2000 to give the reader a comprehensive view of the state of the art. While this is the first review of this topic, a number of important reviews on topics ranging from microfluidic and general analytical methods applicable to metabolomics [1][2][3][4][5][6] to metabolomic and metabolic profiling [7][8][9][10][11][12] have been published previously. For specific topics, it is suggested the reader consult these works. These reviews are by no means a comprehensive list of all reviews in this field given that several dozen review articles have been published over the last nine years ranging in scope from very specific to more general.Before discussing specific applications of microfluidics to metabolomics we will first define metabolomics and the various subsets of this discipline. While many people are familiar with genomics and proteomics, the terminology associated with metabolomics is not as clearly defined. For the sake of this review, we define metabolomics as five separate categories: 1) metabolomics, 2) metabonomics, 3) metabolic fingerprinting, 4) metabolic profiling, and 5) targeted metabolic profiling [13]. Metabolomics is the overall study of metabolite expression Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptAnal Chim Acta. Author manuscript; available in PMC 2010 October 19. Published in final edited form as:Anal Chim Acta. NIH-PA Author ManuscriptNIH-PA Author Manuscript ...

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Chemical cytometry on microfluidic chips

Cited by 23 publications

References 145 publications

Analytical techniques for single-cell metabolomics: state of the art and trends

Analytical techniques for single-cell metabolomics: state of the art and trends

Integrated electrical concentration and lysis of cells in a microfluidic chip

Review: Microfluidic applications in metabolomics and metabolic profiling

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