Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification

Silva, Gildo Almeida da; Bernardi, Taís Letícia; Schaker, Patrícia Dayane Carvalho; Menegotto, Morgana; Valente, Patrícia

doi:10.1590/s1516-89132012000200020

Cited by 71 publications

(50 citation statements)

References 28 publications

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“…The super natant was hosted into a new tube. Quantity et quality of all extract DNA was checked by NanoDrop 1000 Spectrophotometer (Thermo Scientific, USA) and stored at -20° C for next used (Silva et al, 2012).…”

Section: Molecular Diagnostic Methods For the Identification Of Bactementioning

confidence: 99%

Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

Toualbia

Koiche²

2018

Emir J Food Agric

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The main objective of this study is to define probiotic bacteria efficiency against bacterial infantile diarrhea. Lactic acid bacteria (LAB) was used as a natural resource and extracted of camel milk from southern Algeria. The isolation was followed by molecular identification using the 16S rDNA sequencing method. One hundred and fifty fecal samples were collected over 18 months from children suffering from diarrhea and aged 2 years or under. Identification results reveal the isolated lactic bacteria as Lactobacillus plantarum. In the other side, a total of 120 fecal samples were positive for bacterial growth, these pathogenic bacteria were identified as: Escherichia fergusonii (92%), Salmonella enterica subsp. Diarizonae (7.33%) and Proteus mirabilis (0.66%). The inhibitory effect study of lactic acid bacteria on pathogenic bacteria shows varying effects of L. plantarum in relation to the various pathogenic isolates, mentioning that the most important effect was expressed against Escherichia fergusonii with 22 mm. All the results allow us to classify that camel's milk is a natural source rich in lactic acid bacteria, in particular L. plantarum, with an inhibitory ability against the pathogenic bacteria responsible for diarrhea. Therefore L. plantarum produces bioactive molecules responsible for this effect.

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Section: Molecular Diagnostic Methods For the Identification Of Bactementioning

confidence: 99%

Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

Toualbia

Koiche²

2018

Emir J Food Agric

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“…2.5 μL of the supernatant result per 50 μL of the PCR reaction was used. The DNA sample was stored at -20°C, according to da Silva et al (2012).…”

Section: Molecular Identification Of Selected Isolatesmentioning

confidence: 99%

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

Souza¹,

Valentim²,

Nogueira³

et al. 2017

Afr. J. Microbiol. Res.

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Beetles of the Passalidae family live and feed on decaying wood and their guts are richly colonized by yeasts. The goal of this research was to prospect xylolytic yeasts with potential for the production of second-generation bioethanol. Therefore, 83 specimens of beetles belonging to the Passalidae and the Scarabaeidae families were collected in the Amazonian rainforest in Itacoatiara -AM, Brazil. Morphological differences of the beetles were identified and 25 chosen specimens were dissected. Yeasts from galleries inhabited by beetles and from insect guts were isolated. Isolates were previously selected through tolerance tests for temperature, ethanol and xylose assimilation capacity. Those isolates were then submitted to a panel of conditions related to ethanol production. The ethanol production reached 24.70 g.L -1 and the xylitol production reached 21.66 g.L -1. One of the isolates with a promising profile was identified as Spathaspora roraimanensis and six as Spathaspora passalidarum. Three isolates showed to be more promising and, curiously, all came from the gut of the species Popilius marginatus (Percheron, 1835). In plate testing, however, the isolates obtained from galleries showed a greater capacity to assimilate xylose. As reported in this field of study, no isolate tolerated all conditions tested. Wild isolates with this profile may be used for testing larger-scale ethanol production, genetic engineering, or evolutionary techniques.

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“…DNA extractions were carried out using the freeze-thawing process described by other authors (Silva, Bernardi, Schaker, Menegotto, & Valente, 2012). The primer pairs used were ITS1 and ITS4.…”

Section: Pcr-rflp Techniquementioning

confidence: 99%

<b>Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production

Deffert

Agustini

Picheth

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Fructooligosaccharides are catalyzed by β-fructofuranosidase enzyme, produced by many microorganisms. However, in order to achieve a more profitable, low time-consuming process with lower cost, researchers have sought alternatives. This study aimed to select and identify yeasts able to produce fructooligosaccharides and evaluate the influence of pH and temperature on their synthesis. Yeast suspensions, solutions of 500 g L -1sucrose and three values of pH (4.5, 5.5, and 6.5) and temperature (40, 50, and 60ºC) were tested. Yeast species were identified by molecular techniques. Among 141 yeast isolates from grapes, 65 were able to synthesize fructooligosaccharides. The maximum concentration of fructooligosaccharides was 4.8% (w v -1 ), and Saccharomyces cerevisiae 222 produced 1-kestose and nystose.

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Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification

Abstract: ABSTRACT

Cited by 71 publications

References 28 publications

Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

Efficient production of second generation ethanol and xylitol by yeasts from Amazonian beetles (Coleoptera) and their galleries

<b>Screening of whole yeast free-cells and optimization of pH and temperature for fructooligosaccharides production

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