Single particle ICP-MS characterization of platinum nanoparticles uptake and bioaccumulation by Lepidium sativum and Sinapis alba plants

Jiménez-Lamana, Javier; Wojcieszek, Justyna; Jakubiak, Małgorzata; Asztemborska, Monika; Szpunar, Joanna

doi:10.1039/c6ja00201c

Cited by 78 publications

(51 citation statements)

References 44 publications

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“…Classical plant uptake mechanism includes passive spontaneous diffusion, mass flow, ion exchange and active energy intensive carrier-assisted method [14]. In NPs uptake also, similar passive mechanisms were identified in metals (M) [15][16][17], metal oxides (MO) [18,19], chalcogenides (MS) [20], and carbon materials [2,5,21]. Even carrier mediated transport of NPs within the plant cell to different organelles were also documented [22].…”

Section: Introductionmentioning

confidence: 83%

“…The optimum pH range should be 3.5-7.0 for activity of the macerozyme R-10 as provided by the manufacturer; as such, the pH of the citrate buffer was adjusted to 4.5 with citric acid. Then, 2 mL of citrate buffer containing 800 mg of the macerozyme R-10 was added to the above solution, and the samples were incubated for 24 h at 37 °C prior to being digested as reported [16]. After digestion, samples were filtered through 0.45 µm filter paper, and the filtrate was collected.…”

Section: Msmentioning

confidence: 99%

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A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

sharma

Muddassir

Muthusamy

et al. 2020

Preprint

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Background : Classical plant uptake is limited to water soluble or dispersible material. Further, the knowledge of adhesion and uptake of hydrophobic particles is limited to mammalian and bacterial cells, but not studied in plants; so here first time, we tested the fate of hydrophobic particles (oleylamine coated Cu 2-x Se NPs) in comparison to hydrophilic particles (chitosan coated Cu 2-x Se NPs) by treatment on the plant roots and studied the mechanism behind the uptake. Further, Cu 2-x Se NPs are one of the most popular nanomaterial which has been used in various fields viz., electrical, semiconductor industries, biomedicine and in sensing. However, the effects of these NPs on plants have been seldom studied. So here, along with polarity-based uptake, the toxicity of these NPs is investigated in a model plant, tomato. Results : Here, hydrophobic NPs have been found to be ~1.3 times more efficient than hydrophilic NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favourable root trapping and penetration. Further, a relative difference in the hydrophobic vs. hydrophilic NPs movement from the roots to shoots is observed and found related to the change in the protein corona identified by 2D-PAGE analysis. Finally, the toxicity assays showed that Cu 2-x Se NPs lead to non-significant toxicity as compared to control.Conclusions : The enhanced uptake of hydrophobic NPs by the roots proves that non-classical forced penetration is more efficient. Hence, this enhanced uptake and sedentary behaviour of hydrophobic NPs in root can be adopted for eco-friendly leach-proof fertilizer application.

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Section: Introductionmentioning

confidence: 83%

Section: Msmentioning

confidence: 99%

A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

sharma

Muddassir

Muthusamy

et al. 2020

Preprint

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“…4 This indicates that only a single element (isotope) can be monitored using the conventional ICP-MS system (e.g., single particle-ICP-MS: spICP-MS). [5][6][7][8][9] Although fast size distribution analysis of NPs was achieved, analytical restriction still remains, since the system is not capable of monitoring the elemental composition or the isotope ratios of the constituent elements.…”

Section: Introductionmentioning

confidence: 99%

Analytical Capability of High-Time Resolution-Multiple Collector-Inductively Coupled Plasma-Mass Spectrometry for the Elemental and Isotopic Analysis of Metal Nanoparticles

Hirata

Yamashita

Ishida³

et al. 2020

Mass Spectrometry

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We measured the Re/Os (185 Re/ 188 Os) and 187 Os/ 188 Os ratios from nanoparticles (NPs) using a multiple collector-inductively coupled plasma-mass spectrometer equipped with high-time resolution ion counters (HTR-MC-ICP-MS). Using the HTR-MC-ICP-MS system developed in this study, the simultaneous data acquisition of four isotopes was possible with a time resolution of up to 10 µs. This permits the quantitative analysis of four isotopes to be carried out from transient signals (e.g., < 0.6 ms) produced by the NPs. Iridium-Osmium NPs were produced from a naturally occurring Ir-Os alloy (ruthenosmiridium from Hokkaido, Japan; osmiridium from British Columbia, Canada; iridosmine from the Urals region of Russia) through a laser ablation technique, and the resulting nanoparticles were collected by bubbling water through a suspension. The 187 Os/ 188 Os ratios for individual NPs varied significantly, mainly due to the counting statistics of the 187 Os and 188 Os signals. Despite the large variation in the measured ratios, the resulting 187 Os/ 188 Os ratios for three Ir-Os bearing minerals, were 0.121 ± 0.013 for Hokkaido, 0.110 ± 0.012 for British Columbia, and 0.122 ± 0.020 for the Urals, and these values were in agreement with the ratios obtained by the conventional laser ablation-MC-ICP-MS technique. The data obtained here provides a clear demonstration that the HTR-MC-ICP-MS technique can become a powerful tool for monitoring elemental and isotope ratios from NPs of multiple components.

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“…This method is based on ICPMS instruments that allow targeting of inorganic nanoparticles even in a complex matrix by using fast scanning to analyze one particle individually in diluted samples. It has been so far successfully applied to a variety of samples from the environment [3,4], food products [5,6], manufactured goods [7][8][9], or from biological origins [10][11][12][13][14]. Performing an analysis of biological samples by sp-ICPMS requires a digestion step to release the particles from their biological matrix.…”

Section: Introductionmentioning

confidence: 99%

“…At least two methods are currently in use, either an alkaline or an enzymatic digestion. Plant digestions with Macerozyme R-10 (multicomponent enzyme mixture) resulted in a high recovery rate for many different nanoparticles, such as gold nanoparticles [11], but also platinum [12], palladium [15], and cerium oxide nanoparticles [16]. In animal or human tissues, the protease Proteinase K is used.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

Noireaux¹,

Grall

Hullo

et al. 2019

Separations

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Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples.

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Single particle ICP-MS characterization of platinum nanoparticles uptake and bioaccumulation by Lepidium sativum and Sinapis alba plants

Abstract: The uptake and fate of 70 nm PtNPs by two model plants were investigated by enzymatic digestion and SP-ICP-MS analysis.

Cited by 78 publications

References 44 publications

A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

Analytical Capability of High-Time Resolution-Multiple Collector-Inductively Coupled Plasma-Mass Spectrometry for the Elemental and Isotopic Analysis of Metal Nanoparticles

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

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