Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

Noireaux, Johanna; Grall, Romain; Hullo, Marie; Chevillard, Sylvie; Oster, Caroline; Brun, Émilie; Sicard-Roselli, Cécile; Loeschner, Katrin; Fisicaro, Paola

doi:10.3390/separations6010003

Cited by 31 publications

(28 citation statements)

References 35 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relatively short incubation time was particularly helpful to counter potential delays in beam delivery, which are inherent to experiments at accelerator facilities. Previous studies showed that AuNP update and cytotoxicity are cell type dependent, with a preferential uptake by cancer cells in comparison to normal cells ( 62 – 65 ). This brings us to one of the main limitations of the current proof of principle study, since only one non-cancerous cell type was used for this evaluation.…”

Section: Discussionmentioning

confidence: 99%

Radiosensitization Effect of Gold Nanoparticles in Proton Therapy

Cunningham

Kock

Engelbrecht

et al. 2021

Front. Public Health

View full text Add to dashboard Cite

The number of proton therapy facilities and the clinical usage of high energy proton beams for cancer treatment has substantially increased over the last decade. This is mainly due to the superior dose distribution of proton beams resulting in a reduction of side effects and a lower integral dose compared to conventional X-ray radiotherapy. More recently, the usage of metallic nanoparticles as radiosensitizers to enhance radiotherapy is receiving growing attention. While this strategy was originally intended for X-ray radiotherapy, there is currently a small number of experimental studies indicating promising results for proton therapy. However, most of these studies used low proton energies, which are less applicable to clinical practice; and very small gold nanoparticles (AuNPs). Therefore, this proof of principle study evaluates the radiosensitization effect of larger AuNPs in combination with a 200 MeV proton beam. CHO-K1 cells were exposed to a concentration of 10 μg/ml of 50 nm AuNPs for 4 hours before irradiation with a clinical proton beam at NRF iThemba LABS. AuNP internalization was confirmed by inductively coupled mass spectrometry and transmission electron microscopy, showing a random distribution of AuNPs throughout the cytoplasm of the cells and even some close localization to the nuclear membrane. The combined exposure to AuNPs and protons resulted in an increase in cell killing, which was 27.1% at 2 Gy and 43.8% at 6 Gy, compared to proton irradiation alone, illustrating the radiosensitizing potential of AuNPs. Additionally, cells were irradiated at different positions along the proton depth-dose curve to investigate the LET-dependence of AuNP radiosensitization. An increase in cytogenetic damage was observed at all depths for the combined treatment compared to protons alone, but no incremental increase with LET could be determined. In conclusion, this study confirms the potential of 50 nm AuNPs to increase the therapeutic efficacy of proton therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Radiosensitization Effect of Gold Nanoparticles in Proton Therapy

Cunningham

Kock

Engelbrecht

et al. 2021

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Upon completion of all three digestion phases (oral, gastric and intestinal), the total Ag content in both of the IVD AgNPs suspensions and the AgNO 3 solution was similar to that of the corresponding pristine suspensions and solution, except for the highest concentration of both AgNPs, with the total Ag content being $10% and $40% lower for (LA) and (Cit) AgNPs, respectively. The reduction in total Ag content of samples taken from the highest concentrations could be due to a lower (analytical) recovery due to adsorption of AgNPs to the digestion matrix and tubing, and/or to the short centrifugation step applied to the AgNPs samples after digestion to spin down large protein precipitates before ICP-MS measurement (Versantvoort et al 2005) to which the AgNPs could potentially adsorbed (Noireaux et al 2019). This centrifugation set is required to avoid the presence of large proteins that destabilize the plasma of the ICP-MS (Noireaux et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The reduction in total Ag content of samples taken from the highest concentrations could be due to a lower (analytical) recovery due to adsorption of AgNPs to the digestion matrix and tubing, and/or to the short centrifugation step applied to the AgNPs samples after digestion to spin down large protein precipitates before ICP-MS measurement (Versantvoort et al 2005) to which the AgNPs could potentially adsorbed (Noireaux et al 2019). This centrifugation set is required to avoid the presence of large proteins that destabilize the plasma of the ICP-MS (Noireaux et al 2019). On the other hand, a significant reduction in the mass-and number-based AgNPs content was observed for both AgNPs upon completion of all digestion phases, demonstrating that the in vitro digestion could induce dissolution, aggregation, or protein binding of the AgNPs (B€ ohmert et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Impact of in vitro digestion on gastrointestinal fate and uptake of silver nanoparticles with different surface modifications

et al. 2019

View full text Add to dashboard Cite

Nanomaterials, especially silver nanoparticles (AgNPs), are used in a broad range of products owing to their antimicrobial potential. Oral ingestion is considered as a main exposure route to AgNPs. This study aimed to investigate the impact of the biochemical conditions within the human digestive tract on the intestinal fate of AgNPs across an intestinal in vitro model of differentiated Caco-2/HT29-MTX cells. The co-culture model was exposed to different concentrations (250-2500 mg/L) of pristine and in vitro digested (IVD) AgNPs and silver nitrate for 24 h. ICP-MS and spICP-MS measurements were performed for quantification of total Ag and AgNPs. The AgNPs size distribution, dissolution, and particle concentration (mass-and number-based) were characterized in the cell fraction and in the apical and basolateral compartments of the monolayer cultures. A significant fraction of the AgNPs dissolved (86-92% and 48-70%) during the digestion. Cellular exposure to increasing concentrations of pristine or IVD AgNPs resulted in a concentration dependent increase of total Ag and AgNPs content in the cellular fractions. The cellular concentrations were significantly lower following exposure to IVD AgNPs compared to the pristine AgNPs. Transport of silver as either total Ag or AgNPs was limited (<0.1%) following exposure to pristine and IVD AgNPs. We conclude that the surface chemistry of AgNPs and their digestion influence their dissolution properties, uptake/association with the Caco-2/HT29-MTX monolayer. This highlights the need to take in vitro digestion into account when studying nanoparticle toxicokinetics and toxicodynamics in cellular in vitro model systems.

show abstract

“…After euthanasia, each tumor was resected, weighed, and immediately digested through a dissolution in acid for ICP–MS analyses [ 29 , 30 ]. Specifically, the tumor was dissolved in 10 mL of a 3:1 ( υ / υ ) solution of HNO 3 (68%) and H 2 O 2 (50%) and allowed to dissolve overnight.…”

Section: Methodsmentioning

confidence: 99%

Nanoparticle Delivery in Prostate Tumors Implanted in Mice Facilitated by Either Local or Whole-Body Heating

Dockery

Daniel

et al. 2021

Fluids

View full text Add to dashboard Cite

This work discusses in vivo experiments that were performed to evaluate whether local or whole-body heating to 40 °C reduced interstitial fluid pressures (IFPs) and enhanced nanoparticle delivery to subcutaneous PC3 human prostate cancer xenograft tumors in mice. After heating, 0.2 mL of a previously developed nanofluid containing gold nanoparticles (10 mg Au/mL) was injected via the tail vein. The induced whole-body hyperthermia led to increases in tumor and mouse body blood perfusion rates of more than 50% and 25%, respectively, while the increases were much smaller in the local heating group. In the whole-body hyperthermia groups, the IFP reduction from the baseline at the tumor center immediately after heating was found to be statistically significant when compared to the control group. The 1 h of local heating group showed IFP reductions at the tumor center, while the IFPs increased in the periphery of the tumor. The intratumoral gold nanoparticle accumulation was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Compared to the control group, 1 h or 4 h of experiencing whole-body hyperthermia resulted in an average increase of 51% or 67% in the gold deposition in tumors, respectively. In the 1 h of local heating group, the increase in the gold deposition was 34%. Our results suggest that 1 h of mild whole-body hyperthermia may be a cost-effective and readily implementable strategy for facilitating nanoparticle delivery to PC3 tumors in mice.

show abstract

Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS

Cited by 31 publications

References 35 publications

Radiosensitization Effect of Gold Nanoparticles in Proton Therapy

Radiosensitization Effect of Gold Nanoparticles in Proton Therapy

Impact of in vitro digestion on gastrointestinal fate and uptake of silver nanoparticles with different surface modifications

Nanoparticle Delivery in Prostate Tumors Implanted in Mice Facilitated by Either Local or Whole-Body Heating

Contact Info

Product

Resources

About