Single microbead-based fluorescent aptasensor (SMFA) for direct isolation and in situ quantification of exosomes from plasma

Abstract

Exosomes are cell-derived membrane-enclosed biological nanoparticles and carry lots of parental molecular information, recognized as an ideal biomarker for non-invasive diagnosis. However, due to the low abundance of exosomes in...

Application of Single Extracellular Vesicle Analysis Techniques

“… 66 Recently, researchers have developed a new single microbead fluorescent aptamer sensor that incorporates DNA aptamers capable of explicitly binding SEV surface proteins and individual microbeads capable of immunosorbent EVs. 67 This technique allows for the rapid and straightforward detection of 4.9×10 4 vesicles per microliter sample without further signal amplification reactions. Clinical trials have demonstrated the ability of this method to directly detect exosomes from 2 μL plasma samples, showing that the level of CD63, EpCAM double-positive exosomes are higher in cancer patients than in healthy controls.…”

Progress in Nanomaterials-Based Optical and Electrochemical Methods for the Assays of Exosomes

“…Exosome can be labeled with dyes or dye-modified biorecognition elements through various targeting strategies and then determined by fluorescence imaging or spectroscopy. 50,[65][66][67][68][69][70][71][72] However, the low fluorescence intensities of dyes always limits the sensitivity for exosome detection. 73 Thus, several signal amplification strategies have been proposed to improve the detection sensitivity.…”

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

“…13 In particular, various aptasensors consisting of specic aptamers as the recognition element and signal transducer have gained great interest in the liquid biopsy eld, especially for exosome detection. 14,15 Besides recognizing exosomes, the aptasensors can be combined with various signal transduction technologies including colorimetry, 16,17 electrochemistry, [18][19][20] electro-generated chemiluminescence, 21 uorescence, [22][23][24][25] and Surface-Enhanced Raman Scattering (SERS) 26,27 to measure the concentration of the isolated exosomes. Among them, colorimetric aptasensors have been widely applied to detect exosomes due to their low cost, simplicity and observation with naked eyes, which is oen based on natural peroxidases or peroxidase mimetics, such as horseradish peroxidase and carbon nanomaterials.…”