Single cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice

Abstract

Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory neurons in the preBötC of neonatal mouse slices that retain breathing-related rhythmicity in vitro. We classified neurons based on their electrophysiological properties and genetic background, and then aspirated their cellular contents for single-cell RNA sequencing (scRNA-seq). This data set provides the raw nucleotide sequences (FASTQ files) and annotated files of nucleotide sequences mapped to the mouse genome (mm10 from Ensembl), which includes the fragment counts, gene lengths, and fragments per kilobase of transcript per million mapped reads (FPKM). These data reflect the transcriptomes of the neurons that generate the rhythm and pattern for inspiratory breathing movements.

Role of NaV1.6-mediated persistent sodium current and bursting-pacemaker properties in breathing rhythm generation

“… 19 , 46 , 47 Therefore, we confined our analysis to the role of the I NaP , targeting Na v 1.6 channels encoded by Scn8a that constitute the I NaP in many neuron types, 26 , 28 , 29 including core preBötC neurons. 15 , 33 , 35 …”

“… 26 – 32 Deletion of Scn8a causes a 70%–76% reduction in the I NaP in null mice. 28 , 31 Therefore, although Na V 1.1, Na V 1.2, Na V 1.3, Na V 1.6, and Na V 1.9 are expressed in rhythmogenic preBötC neurons, 15 , 33 – 35 Na V 1.6 ( Scn8a ) is the most promising candidate target for genetic manipulation to evaluate the role of the I NaP and of bursting-pacemaker properties in rhythmogenesis.…”