Single cell oil production by Trichosporon cutaneum and lignocellulosic residues bioconversion for biodiesel synthesis

Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis

“…xylose) and inhibitors (5-hydroxymethylfurfural (HMF), furfural, and acetic acids) was determined using HPLC (Agilent Technology 1260 Infinity, refractive index detector RID, Agilent, USA) equipped with a Bio-Rad Aminex HPX-87H column. Sulfuric acid at 5 mM was used as a mobile phase with a flow rate of 0.6 mL/min, and at the column, temperature was at 65°C [9]. All compounds analyzed are used as standard at a concentration of 1 g/L.…”

“…Lipid production was improved only in 3-liter bioreactor batch fermentation using glucose/xylose 2 : 1 combination [36]. On the other hand, the use of the glucose/ xylose combination as a carbon source significantly improves the lipids production by other oleaginous yeast strains such as C. viswanathii Y-E4 and T. cutaneum CTM-30125 (4.28 g/L and 4.58 g/L, respectively) [9,23]. Also, we noticed that Y-MG1 was able to assimilate and synthesize lipids when grown on glycerol (lipid content of 27.08%).…”

“…Nevertheless, the polyunsaturated fatty acids did not exceed 8%. is lipid composition is highly similar to L. starkeyi and T. cutaneum when grown on a corncob and barley hulls acid hydrolysates, respectively [9,55]. is composition, largely identical to that of many vegetable oils, was considered as ideal for biodiesel synthesis [56].…”

“…ey have a short duplication times, a unicellular form, and are very easy to grow in controlled and intensive systems [2]. In addition, one of the most important characteristics of yeast resides in its ability to use a large variety of raw materials for lipid production such as industrial wastewater [5], municipal organic wastes [6], crude glycerol [7], and lignocellulosic acid hydrolysate [8,9]. e most known genera of oleaginous yeast are Candida, Cryptococcus, Lipomyces, Rhodotorula, Trichosporon, and Yarrowia [10].…”

“…In order to increase the bioconversion rate of lignocellulosic biomass, several detoxification processes have been developed to eliminate and reduce toxic compounds derived from the hydrolysis step [17]. e overliming using the calcium hydroxide and the combination of overliming and adsorption using the activated charcoal or the amberlite resin are the most studied of chemical and physical detoxification methods [1,9]. ese techniques have many disadvantages such as the massive freshwater usage and wastewater generation, loss of the fine lignocellulose particles and fermentative sugars, and the incomplete removal of inhibitors [15].…”