Simvastatin inhibits human saphenous vein neointima formation via inhibition of smooth muscle cell proliferation and migration

Abstract

Please refer to abstract number 61. This abstract was originally submitted as a poster, and on the basis of its scientific interest and merit, was chosen by the colloquium organizers to be presented as an oral communication, as well as a poster.Please refer to abstract number 52. This abstract was originally submitted as a poster, and on the basis of its scientific interest and merit, was chosen by the colloquium organizers to be presented as an oral communication, as well as a poster.

Arterial pO2 stimulates intimal hyperplasia and serum stimulates inward eutrophic remodeling in porcine saphenous veins cultured ex vivo

“…To conduct extensive studies on the role of pO 2 in a timely manner, we adopted a much simpler non-perfused SV organ culture system widely used by others (Soyombo et al 1990;Porter et al 1996bPorter et al , 1998aPorter et al ,b, 1999Porter et al , 2001Porter et al , 2002Jeremy et al 1997;Corpataux et al 2005;Mekontso-Dessap et al 2006). Porcine vessels from pigs 60-65 kg weight were cultured inside sterile 100-mm plastic Petri dishes (Nalgene Nunc, Fisher Scientific), pre-coated with ∼7 mm of Sylgard 184 resin (Dow Corning Corporation, USA).…”

“…Here we conducted experiments to explore a third possibility-i.e., non-mechanical aspects of the ex-vivo culture system might account for some of the observed changes in cultured vessels. Saphenous veins (SV) are attractive vessels to use for these studies because (1) they are well studied in various ex-vivo culture systems (Porter et al 1996a(Porter et al ,b, 1998a(Porter et al ,b, 1999(Porter et al , 2001(Porter et al , 2002Gusic et al 2005a,b), (2) they exhibit pronounced changes in culture which do not appear to be due to the mechanical environment (Porter et al 1996b(Porter et al , 1999, and (3) their remodeling is an important component of both the success and failure of vein grafts used in coronary artery bypass grafting and peripheral revascularization (Bourassa 1991;Motwani and Topol 1998).…”

Choroidal Vessel Wall: Hypercholesterolaemia-Induced Dysfunction and Potential Role of Statins

“…Statin administration is able to attenuate these effects of both small G-protein families [140]. Simvastatin inhibits both VSMC proliferation and migration in a series of experiments with human saphenous vein grafts [149].…”

The Effect of Lowering LDL Cholesterol on Vascular Access Patency

“…The venous stenoses that result are characterized by the proliferation of smooth muscle cells with a myofibroblastic phenotype, as well as their migration (with macrophages) into the vessel's intima (16)(17)(18). Statin-based therapy might slow progression of neointimal hyperplasia by reducing vascular smooth muscle cell proliferation (19)(20)(21)(22)(23), smooth muscle cell migration (22), and monocyte activation (24), effects that are not known to be mediated through the LDL-C-lowering effect of hepatic hydroxymethyl glutaryl-CoA reductase inhibition.…”