Simultaneous quantitation of enalapril and enalaprilat in human plasma by 96‐well solid‐phase extraction and liquid chromatography/tandem mass spectrometry

Abstract

A sensitive and rapid method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) combined with rapid solid-phase extraction (SPE) has been developed and validated for the quantitative determination of enalapril and its active metabolite enalaprilat in human plasma. After addition of internal standard to human plasma, samples were extracted by 96-well SPE cartridge. The extracts were analyzed by HPLC with the detection of the analyte in the multiple reaction monitoring (MRM) mode. This method for the simultaneous determination of enalapril and enalaprilat was accurate and reproducible, with respective limits of quantitation of 0.2 and 1.0 ng/mL in plasma. The standard calibration curves for both enalapril and enalaprilat were linear (r(2) = 0.9978 and 0.9998) over the concentration ranges 0.2-200 and 1.0-100 ng/mL in human plasma, respectively. The intra- and inter-day precision over the concentration range for enalapril and enalaprilat were lower than 13.3 and 15.4% (relative standard deviation, %RSD), and accuracy was between 89.2-105.0 and 91.9-104.7%, respectively.

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

“…In many cases, however, this is not true and lopsided concentration profiles can be observed, especially for prodrugs that typically have much shorter halflife than the active drug. This phenomenon has been observed by Lee et al [141].…”

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

“…[1][2][3] Different analytical methods have been applied to the determination of the remaining drugs analyzed by us, and also by HPLC techniques. Many of the HPLC analytical methods exist for the assay of ENA, [4][5][6][7] SOT, [8][9][10][11][12][13][14][15] CAP, [16][17][18][19][20][21][22][23][24] MET, [25][26][27][28][29][30][31] 62,63 and SPI [64][65][66][67][68] in different biological fluids and tissues. HPLC methods were applied to the simultaneous quantification of selected β-blockers (also: SOT, MET and PRO) in human fluids (plasma, serum, urine).…”

Определение Эналаприла И Его Активного Метаболита Эналаприлата В Плазме Крови Методом Вэжх-Мс/Мс При Персонализации Фармакотерапии Больных Артериальной Гипертензией

“…Ñðåäè ñîâðåìåííûõ àíàëèòè÷åñêèõ ìåòîäîâ îïðå-äåëåíèÿ ýíàëàïðèëà è ýíàëàïðèëàòà â ïëàçìå èëè ñû-âîðîòêå êðîâè ÷åëîâåêà áîëüøèíñòâî àâòîðîâ îòäà¸ò ïðåäïî÷òåíèå ÂÝAEÕ-ÌÑ/ÌÑ ââèäó âûñîêîé ÷óâñòâè-òåëüíîñòè è ñåëåêòèâíîñòè ìåòîäà [6,7,9,10,13].  êà÷åñòâå ñïîñîáà ïðîáîïîäãîòîâêè äëÿ îáðàçöîâ ïëàç-ìû êðîâè ìîaeíî èñïîëüçîâàòü îñàaeäåíèå áåëêîâ àöå-òîíèòðèëîì [6,7], aeèäêîñòü-aeèäêîñòíóþ ýêñòðàêöèþ [6,9,10] è òâåðäîôàçíóþ ýêñòðàêöèþ [13].…”

“… êà÷åñòâå ñïîñîáà ïðîáîïîäãîòîâêè äëÿ îáðàçöîâ ïëàç-ìû êðîâè ìîaeíî èñïîëüçîâàòü îñàaeäåíèå áåëêîâ àöå-òîíèòðèëîì [6,7], aeèäêîñòü-aeèäêîñòíóþ ýêñòðàêöèþ [6,9,10] è òâåðäîôàçíóþ ýêñòðàêöèþ [13].…”