Simultaneous quantification of picfeltarraenins IA and IB in rat plasma by UPLC–MS/MS: Application to a pharmacokinetic study

He, Xin; Zhang, Yingjie; Hang, Gao; Li, Keyan; Zhang, Yazhou; Sun, Limin; Gao, Tao

doi:10.1016/j.jpba.2015.11.031

Cited by 7 publications

(3 citation statements)

References 4 publications

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“…The main determination method is high-performance liquid chromatography (HPLC), [10][11][12][13] followed by a few thin-layer chromatography (TLC) methods. The fly in the ointment is that they have some limitations in practical applications.…”

Section: Relevant Pharmacological Experiments Indicated That Extracts Of Picriaementioning

confidence: 99%

“…He et al 10 established an ultra-performance liquid chromatography method coupled with tandem mass spectrometry (UPLC-MS/MS) to realize the simultaneous quantification of picfeltarraenins IA and IB in rat plasma. Fang et al 11 developed an HPLC method to achieve the quantification of picfeltarraenins IA and IB in P. fel-terrae Lour.…”

Section: Relevant Pharmacological Experiments Indicated That Extracts Of Picriaementioning

confidence: 99%

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Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Wang

Yang

Guo

et al. 2021

Phytochemical Analysis

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Introduction: Picfeltarraenins IA, IB and IV and acteoside are the four bioactive ingredients of Picria fel-terrae Lour. Their pharmacological effects include central inhibitory, cardiovascular, anti-inflammatory, anti-pyretic, analgesic, anti-bacterial, antioxidative and anti-tumor effects.Objective: We aimed to develop an efficient micellar electrokinetic chromatography (MEKC) method modified with mixed organic solvents for the simultaneous separation and determination of the four components in Picriae Herba and its formulations.Methods: Method optimization was carried out by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of the four components in Picriae Herba and its formulations.Results: The optimal running buffer was composed of 20 mM sodium tetraborate, 40 mM sodium cholate, 10% (v/v) methanol and 10% (v/v) isopropanol (pH 9.76).The separation voltage was 18 kV, the temperature was 25 C and the detection wavelength was 266 nm. Under the optimal separation conditions, the baseline separation of four components was achieved in less than 14 min. The correlation coefficients of the calibration curves were 0.9984-0.9995 for the analytes. The intraday and interday precision ranged from 1.5% to 2.5% and from 1.4% to 5.0%, respectively. Recoveries of analytes varied from 96.6% to 104.1%. Conclusion:The method was proved suitable for the determination of four components in Picriae Herba and its formulations. Good performance was obtained under optimal conditions, and the method provides an effective tool for the quality control of Picriae Herba and its formulations.

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Section: Relevant Pharmacological Experiments Indicated That Extracts Of Picriaementioning

confidence: 99%

Section: Relevant Pharmacological Experiments Indicated That Extracts Of Picriaementioning

confidence: 99%

Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Wang

Yang

Guo

et al. 2021

Phytochemical Analysis

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“…Pharmacokinetics studies on ESL active ingredients would be helpful to study the efficacy and toxicity of ESL. At present, there are no pharmacokinetic studies on saponins from ESL have been reported, mainly because the molecular weight of saponins is large and it is difficult to detect in plasma [24]. In this study, a highly sensitive, novel method for simultaneously determination of the six triterpenoid saponins in Beagle dog plasma was established by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of six triterpenoid saponins in beagle dog plasma by UPLC-MS/MS and its application to a pharmacokinetic study after oral administration of the extract of the Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves

Wang

Chang

Feng

et al. 2023

AChrom

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A rapid and simple ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for simultaneous determination of six analytes from the Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves (ESL) in beagle dog plasma for the first time, including 3-O-α-l-rhamnopyranosyl-(1→2)-α-l-arabinopyranoside-29-hydroxy oleanolic acid, 3-O-β-d-glucopyranosyl-(1→2)-α-l-arabinopyranoside-29-hydroxy oleanolic acid, 3-O-β-d-glucopyranosyl-(1→2)-α-l-arabinopyranosyl-30-norlean-12,20 (29) –dien-28-olic acid, ciwujianoside E, guaianin N, and eleutheroside K. The chromatographic separation was performed using an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) using a gradient elution way with a mobile phase of acetonitrile-water containing 0.1% formic acid. Analytes were detected on a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source with multiple reaction monitoring (MRM) mode. Calibration curves were all linear (r ≥ 0.9933) over the concentration range. The mean extraction recoveries and matrix effect of analytes and I.S. were ranged from 80.26% to 98.32% and from 91.27% to 111.67%, respectively. The intra-day and inter-day precision were ranged from 2.20% to 14.81%, and the accuracy range was 1.60–14.60%. The analytical method was successfully applied for the pharmacokinetic characteristics of the six analytes in beagle plasma after oral administration of ESL extracts. The T 1/2 of six analytes was more than 3.09 ± 0.78 h.

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Determination of 25-OCH3-PPD and the related substances by UPLC–MS/MS and their cytotoxic activity

Ding

Zhao

et al. 2016

Journal of Chromatography B

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Simultaneous quantification of picfeltarraenins IA and IB in rat plasma by UPLC–MS/MS: Application to a pharmacokinetic study

Cited by 7 publications

References 4 publications

Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Simultaneous separation and determination of four active ingredients in Picria fel‐terrae Lour. and its preparations by micellar electrokinetic chromatography

Simultaneous determination of six triterpenoid saponins in beagle dog plasma by UPLC-MS/MS and its application to a pharmacokinetic study after oral administration of the extract of the Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves

Determination of 25-OCH3-PPD and the related substances by UPLC–MS/MS and their cytotoxic activity

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