Poor sleep quantity and quality may predispose FI adults to adverse health outcomes.

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MicroRNAs (MiRs) are thought to display regulator action in tumor suppression and oncogenesis. miR-144 plays an important role in the development of various cancers, such as colorectal cancer, breast cancer, and lung cancer, by targetting different molecules potentially involved in many signaling pathways. SMAD4 is a common signaling during tumor progression, and it can inhibit cell proliferation and promote cell motility in most epithelial cells. The present study focused on the effect of miR-144 and SMAD4 on colon cancer in order to find the novel gene therapy target for the treatment of colon cancer. Quantitative real-time polymerase chain reaction was used to assess the expression level of miR-144 in colon cancer tissues and SW620 cells. MTT assay, scratch test, and transwell assay were used to evaluate cell proliferation, migration, and invasion, respectively. Moreover, luciferase assays were utilized to identify the predictive effect of miR-144 on SMAD4. Western blotting was performed to determine the relative expression of protein related to SMAD4. We found miR-144 level was significantly lower in colon cancer tissues and SW620 cells. Moreover, SMAD4 level, both in mRNA and protein, was obviously elevated in colon cancer tissues. Further, miR-144 mimics treatment inhibited cells proliferation, invasion, and migration. Fluorescence intensity of miR-144 mimics group in wild type cells was decreased. MiR-144 mimics repressed the SMAD4 expression both in mRNA and protein. These findings about miR-144/SMAD4 pair provide a novel therapeutic method for colon cancer patients.

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Conventional capillary number theory predicts that residual oil will not be mobilized until a critical capillary number (2E - 5) is exceeded. This theory was tested to determine residual oil saturation mobilization after forced water imbibition. In the literature, high residual oil saturation was established from waterflooding at a certain pressure. Then gradually increasing pressure was applied for water injection until residual oil production was observed. It was confirmed that this critical capillary number is applicable when the initial residual oil saturation was estimated from spontaneous water imbibition tests. The same types of experiments were repeated for gas-liquid systems by creating the initial residual gas saturation through spontaneous oil or water imbibition tests on the same samples. The procedures, which included spontaneous imbibition tests followed by forced imbibition tests, were monitored by an on-line NMR system. Analysis of the experimental results produced an estimate of the critical capillary number for mobilizing residual gas from water imbibition or oil imbibition tests. In this research, we broadened the term of "imbibition" for both water and oil terms since, compared to gas, both water and oil are in the wetting phase. It was found that the critical capillary number for a gas-liquid system is very different from that of an oil-water system in the same type of rock. Berea sandstone plugs were used in all the experiments. The reason for using Berea sandstone plugs is due to their relatively homogeneous pore structure. Additional Western Canadian sandstone plugs were used for testing gas-water systems to confirm the results obtained from the Berea sandstone plugs. Understanding the different mechanisms to produce discontinuous residual oil or residual gas is important for enhanced oil and gas recovery operations. Hopefully, this research can provide new insights into recovering additional gas from gas reservoirs with active aquifers. Introduction Increasing the capillary number has long been investigated as a strategy for improving oil recovery. Many methodologies around increasing the capillary number have been either tested in the laboratory or applied in the field. Capillary number is defined as the ratio of viscous forces to capillary forces. Evaluation of the capillary number can be used to describe the relative importance of viscous forces to capillary forces during immiscible displacements. There are various forms of the capillary number. The most common versions of capillary number are those by Saffman and Taylor(1): Equation (1) (Available in full paper) and Melrose and Brandner(2): Equation (2) (Available in full paper) When non-wetting phase oil is trapped in porous media, the pressure gradient required to move it through a capillary tube is much higher than what would be predicted by the pipe flow equation, due to the pressure discontinuity at the wetting/non-wetting interface. Because of the contact angle hysteresis, this discontinuity is not of the same magnitude on both sides of the discontinuous non-wetting phase. For example, in a water-wet medium, an oil droplet represents the discontinuous non-wetting phase. As the oil droplet is pushed through a pore throat, its downstream end gets squeezed into a much narrower segment making its radius of curvature much smaller than the upstream part.