Simultaneous quantification of flavonoids and triterpenoids in licorice using HPLC

Wang, Yuan-Chuen; Yang, Yi-Shan

doi:10.1016/j.jchromb.2006.12.032

Cited by 109 publications

(82 citation statements)

References 20 publications

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“…Various chromatographic methods, including high performance liquid chromatography with a photo diode array detector (HPLC-PDA), have been used to separate, identify and quantify chemical markers in plant extracts, which in general have a complex chemical composition, besides the presence of interfering compounds (Wang & Yang, 2007). A previous study (Leal et al, 2000) carried out in our laboratory determined the preliminary phytochemical profile of "chambá", detecting the presence of coumarin among other constituents of the plant.…”

Section: Discussionmentioning

confidence: 99%

Justicia pectoralis Jacq: , Acanthaceae: preparation and characterisation of the plant drug including chromatographic analysis by HPLC-PDA

Fonseca¹,

Silva²,

Leal³

2010

Rev. bras. farmacogn.

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RESUMO:Justicia pectoralis Jacq., Acanthaceae, é uma erva conhecida popularmente no Nordeste como chambá e, utilizada tradicionalmente no tratamento de doenças do trato respiratório, como a asma, tosse e bronquite. Essa espécie encontra-se na Relação Nacional de Plantas Medicinais de Interesse para o SUS. O objetivo do presente estudo foi elaborar protocolo para a preparação da droga vegetal a partir de J. pectoralis e realizar a sua caracterização visando seu emprego como matéria-prima farmacêutica. A parte aérea de J. pectoralis, após secagem em estufa com circulação e renovação de ar (35 °C) durante diferentes períodos de tempo (1-5 dias) mostrou a partir de 24 h de secagem um teor de umidade abaixo do valor máximo permitido para drogas vegetais. O pó da droga vegetal foi classificado como pó moderadamente grosso e, caracterizado quanto aos teores de cinzas totais, extrativos solúveis em água e etanol. Análise do extrato hidroalcoólico (etanol 20%) de J. pectoralis por Cromatografia Líquida de Alta Eficiência (CLAE-DAD) determinou um teor de cumarina e umbeliferona de 16,2 e 0,81 mg/g da droga vegetal, respectivamente. As condições de preparação da droga vegetal e os parâmetros de controle de qualidade determinados para J. pectoralis no presente estudo são de interesse no desenvolvimento de fitoterápicos que empreguem esse matéria-prima ativa.Unitermos: Justicia pectoralis, cumarina, umbeliferona, CLAE-DAD, controle de qualidade, matéria-prima vegetal.ABSTRACT: Justicia pectoralis Jacq., Acanthaceae, is a herb popularly known in Brazilian northeast as "chambá" and used in folk medicine for the treatment of respiratory tract conditions such as asthma, cough and bronchitis. This species is included in the National Register of Plants of Interest to the National Health System. The aim of the present study was to develop a protocol for the preparation of the plant drug from J. pectoralis and to characterise the plant drug for its use as a pharmaceutical raw material. The aerial parts of J. pectoralis, after drying chamber with forced air circulation (35 °C) for different periods of time (1-5 days), presented after one day a moisture content below the maximum allowed for plant drugs. The powder of the plant drug was classified as moderately coarse, and the total ashes content and the water-or ethanolsoluble extractives were determined. Analysis of hydroalcoholic (ethanol 20%) extract of J. pectoralis by high performance liquid chromatography-photo diode array (HPLC-PDA) determined the content of coumarin and umbelliferone (16.2 and 0.81 mg/g plant drug, respectively). The preparation conditions of the plant drug and the quality control parameters established for J. pectoralis in this study are of interest for the development of phytomedicines which use this active raw material.Keywords: Justicia pectoralis, coumarin, umbelliferone, HPLC-PDA, quality control, plant raw material. Justicia pectoralis Jacq., Acanthaceae: preparation and characterisation of the plant drug including chromatographic analysis by HPLC-PDA

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Section: Discussionmentioning

confidence: 99%

Justicia pectoralis Jacq: , Acanthaceae: preparation and characterisation of the plant drug including chromatographic analysis by HPLC-PDA

Fonseca¹,

Silva²,

Leal³

2010

Rev. bras. farmacogn.

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“…It appears in approximately 60% of all TCM prescriptions (Wang and Yang, 2007). Licorice is derived from the roots and rhizomes of Glycyrrhiza uralensis, Glycyrrhiza inflata, and Glycyrrhiza glabra (Chinese Pharmacopoeia Commission, 2010) and is mainly used to treat peptic ulcer, cough, and hepatitis C (Asl and Hosseinzadeh, 2008).…”

Section: Introductionmentioning

confidence: 99%

From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats

Cheng¹,

Qiao²,

Wang³

et al. 2011

Drug Metab Dispos

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ABSTRACT:Because of the complicated chemical composition of traditional Chinese medicines (TCMs), their metabolic study has been a great challenge, especially when they are used in the traditional way, i.e., clinical oral dose of water decoction. Poor understanding of metabolic pathways and too low metabolite concentrations to be detected in biosamples are the major hurdles. In the present work, a three-step strategy was proposed to systematically characterize in vivo metabolites of TCMs at a normal clinical dosage. Licorice, one of the most popular TCMs, was studied as a model. First, 10 representative compounds of licorice were administered to rats separately. A total of 68 metabolites were characterized by highperformance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry and liquid chromatography (LC)/quadrupole time-of-flight-mass spectrometry (MS) analyses, together with enzyme hydrolysis. Among these, 13 compounds were confirmed by comparison with reference standards, including the 10 administered licorice compounds. Second, a high dose (equivalent to 20-fold clinical dosage) of licorice water extract was administered, and 22 more metabolites were characterized. Finally, these metabolites (including constituents of licorice) were determined by a highly sensitive and selective LC/selected reaction monitoring-MS method when the licorice water decoction was orally administered to rats at a clinical dosage (0.9 g crude drug/kg). A total of 42 metabolites in plasma and 62 metabolites in urine were detected. This is the first attempt to fully profile the in vivo metabolites of licorice at a normal clinical dosage.

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“…8 Therefore, developing a sensitive and reliable analytical method not only for the characterization of the multiple compounds in licorice, but more importantly for the detection of which compounds are absorbed in plasma after oral administration, is a great need. Many analytical methods have been used for the analysis of phenolic and triterpenoid compounds in licorice, including high-performance liquid chromatography diode-array detection (HPLC/DAD), 9,10 high-performance liquid chroma-(QITMS) can distinguish isomers according to their characteristic fragment ions. 16,17 So chemical compounds in CHMs can be identified or tentatively characterized by combining the two techniques.…”

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confidence: 99%

Analysis of phenolic and triterpenoid compounds in licorice and rat plasma by high‐performance liquid chromatography diode‐array detection, time‐of‐flight mass spectrometry and quadrupole ion trap mass spectrometry

Tan

Zhu

Hai

et al. 2009

Rapid Comm Mass Spectrometry

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High-performance liquid chromatography with diode-array detection (HPLC/DAD), time-of-flight mass spectrometry (HPLC/TOFMS) and quadrupole ion trap mass spectrometry (HPLC/QITMS) were used for separation and identification of several compounds in licorice and rat plasma after oral administration of the herbal extract. Three phenolic compounds and one triterpenoid in licorice extract were unambiguously identified by comparing with the standard compounds. A formula database of known compounds in licorice was established, against which the other 42 compounds were identified effectively based on the accurate extract masses and formulae acquired by HPLC/TOFMS. In order to differentiate the isomers, tandem mass spectrometry was also used. The deduced fragmentation behaviors in QITMS were used to distinguish seven groups of isomers in licorice. By means of the three detectors, 46 compounds in licorice were identified. After oral administration of the extract, 25 compounds in rat plasma were detected and identified by comparing and contrasting the compounds measured in licorice with those in the plasma samples by HPLC/TOFMS. It is concluded that a rapid and effective method based on three analytical techniques was established, which is useful for identification of multiple compounds in licorice in vitro and in vivo. The result should be very useful for the quality control and curative mechanism study of licorice.

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Simultaneous quantification of flavonoids and triterpenoids in licorice using HPLC

Cited by 109 publications

References 20 publications

Justicia pectoralis Jacq: , Acanthaceae: preparation and characterisation of the plant drug including chromatographic analysis by HPLC-PDA

Justicia pectoralis Jacq: , Acanthaceae: preparation and characterisation of the plant drug including chromatographic analysis by HPLC-PDA

From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats

Analysis of phenolic and triterpenoid compounds in licorice and rat plasma by high‐performance liquid chromatography diode‐array detection, time‐of‐flight mass spectrometry and quadrupole ion trap mass spectrometry

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