Simultaneous Monitoring of Aspirin, Paracetamol and Caffeine in Human Urine at Poly-1,5-diaminonapthalene Modified Pyrolytic Graphite Sensor

Abstract

A simple and highly sensitive method based on poly-1,5-diaminonapthalene (p-DAN) modified pyrolytic graphite sensor (MPGS) is delineated for the simultaneous determination of acetylsalicylic acid (ASA), paracetamol (PAR) and caffeine (CF) in nanomolar concentrations in human urine. The electrooxidation of ASA, PAR and CF occurred in well defined peaks at pH 7.2. After optimization of analytical conditions exploiting this sensor, the peak currents for the three compounds were found to increase linearly with increase in their concentration in the range of 0.1-120 nM and detection limits of, 0.93 × 10 −10 , 0.57 × 10 −10 and 0.64 × 10 −10 M were observed for ASA, PAR and CF respectively. The sensor was also successfully employed for the simultaneous determination of all the three compounds in human urine and pharmaceutical samples using square wave voltammetry (SWV). As a conducting polymer modified sensor has been used, several advantages, such as a very low detection limits, high sensitivity, long stability and excellent reproducibility were observed. The method is selective and all the three compounds can be determined effectively in human urine samples without any interference from common urine metabolites such as uric acid and ascorbic acid.

Synthesis of poly(8-aminopyrene-1,3,6-trisulfonic acid)/CNT Nanocomposite for Electrochemical Detection of Caffeine

“…1 In addition, electrochemical sensors are highly sensitive and selective for oxidation of CAF. 6 Recently, poly(4-amino-3-hydroxynaphthalene sulfonic acid), 7 graphene-Nafion composite film, 8 SWCNTs modified carbon-ceramic electrode, 9 electrochemically reduced graphene oxide, 10 and Nafion-modified film, 11 bismuth particles/Nafion covered boron-doped diamond electrode, 12 and poly(Nile blue) modified modified glassy carbon electrodes (GCE) were reported for CAF detection. 13 Single-walled carbon nanotubes (SWCNTs) offer several unique functional properties such as high specific surface area, conductivity, mechanical strength, and excellent thermal properties.…”

A Simple and Sensitive Poly‐1,5‐Diaminonaphthalene Modified Sensor for the Determination of Sulfamethoxazole in Biological Samples

“…The application of p‐DAN conducting polymer has been studied at different surfaces, such as platinum and glassy carbon, pyrolytic graphite electrode with gold nanoparticles and glassy carbon electrode with multiwalled carbon nanotube in the selective monitoring of compounds 35, 36. p‐DAN has been considered as a promising candidate to form polymerdrug conjugate for drug delivery purposes and it is successfully applied for the determination of various drugs as earlier reported by our group 37–39. Here, we used only p‐DAN film to fabricate the electrode due the some of the advantages, such as easy synthesis, low cost in comparison to other conducting polymers, good adhesive property and dense structure 40–42.…”