Simultaneous linear release of folic acid and doxorubicin from ethyl cellulose/chitosan/g‐C₃N₄/MoS₂ core‐shell nanofibers and its anticancer properties

Abstract

The folic acid (FA) and doxorubicin (DOX) have been doped into the g‐C3N4/MoS2 incorporated‐chitosan/ethyl cellulose (EC) core‐shell nanofibers for targeted delivery of FA and DOX against HeLa and MCF‐7 cell lines. The g‐C3N4/MoS2 nanosheets and core‐shell nanofibers were characterized using Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, and UV–Vis tests. The drug loading factor, the degradation rate, and the DOX and FA release behavior from core‐shell nanofibers have been investigated. The pharmacokinetic results revealed the linear release with non‐Fickian diffusion of the both anticancer drugs from nanofibers during 7 days. The DAPI staining and MTT assays of the nanofibers immersed in MCF‐7 and HeLa cell lines were studied to determine the potential of DOX and FA doped‐core‐shell nanofibrous matrix for MCF‐7 and HeLa cells death in vitro. The maximum MCF‐7 and HeLa cells death percentages were found to be 89 and 85%, respectively, using EC/chitosan/g‐C3N4/MoS2/DOX/FA core‐shell nanofibers after 7 days. The high activity of g‐C3N4/MoS2/DOX/FA loaded‐core‐shell nanofibers for studied cancer cells killing was achieved.

Advancements in Fabrication and Application of Chitosan Composites in Implants and Dentistry: A Review

“…Zn 2+ , Cu 2+ , Mg 2+ , and Sr 2+ are a few metal ions to name. Some techniques such as sol-gel, electrospinning, freeze-drying, and complexation interactions are used to fabricate composites of chitosan-metal [ 103 , 112 , 113 , 114 , 115 , 116 ]. A chitosan-silver composite scaffold was developed by Vaidhyanathan et al by utilizing a simple freeze-dry method.…”

Preparation of bioactive polymer-based composite by different techniques and application in tissue engineering: A review

“…The electrospinning method comprises three sections: a material transfer system (regularly a thin tube including a spinneret of miniature diameter); a high voltage power system; and a fiber collection system [137]. The electrospinning method uses an elevated voltage to generate an electrically charged melt jet outside the control system or polymer solution.…”

Synthesis, characterization, and biological activities of folic acid conjugates with polyvinyl alcohol, chitosan, and cellulose

“…48 Ethyl CL/CS/g-C 3 N 4 /MoS 2 core-shell nanofibers were utilized as carriers for linear release of FA and doxorubicin to treat the MCF-7 and HeLa cancer cell lines. 49 Deoxycholic acid-Ocarboxymethylated CS-FA conjugates were constructed by the modification of O-carboxymethylated chitosan with deoxycholic acid, and then covalently bound with FA to develop a novel cancer-targeted drug delivery system. 50 Self-organized nanoparticles based on CS-FA and dextran succinate-doxorubicin conjugates showed promising activities for anticancer drug targeting.…”

“…In addition, PLGA‐PEG‐FA nanoparticles carrying 17‐AAG were evaluated in MCF‐7 human breast cancer cells and showed enhancing in the cytotoxicity of the 17‐AAG 48 . Ethyl CL/CS/g‐C 3 N 4 /MoS 2 core‐shell nanofibers were utilized as carriers for linear release of FA and doxorubicin to treat the MCF‐7 and HeLa cancer cell lines 49 . Deoxycholic acid–O‐carboxymethylated CS‐FA conjugates were constructed by the modification of O‐carboxymethylated chitosan with deoxycholic acid, and then covalently bound with FA to develop a novel cancer‐targeted drug delivery system 50 .…”