Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

Hanson, Cynthia; Barney, Jacob T; Bishop, Morgan M.; Vargis, Elizabeth

doi:10.1002/elps.201800389

Cited by 21 publications

(23 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to a well‐developed predictive model, clinical samples require some form of preparation prior to analysis. This work is already underway in our lab using a single platform that incorporates dielectrophoresis for bacterial isolation and Raman spectroscopy for identification .…”

Section: Resultssupporting

confidence: 92%

Effect of growth media and phase on Raman spectra and discrimination of mycobacteria

Hanson

Bishop

Barney

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

When developing a Raman spectral library to identify bacteria, differences between laboratory and real world conditions must be considered. For example, culturing bacteria in laboratory settings is performed under conditions for ideal bacteria growth. In contrast, culture conditions in the human body may differ and may not support optimized bacterial growth. To address these differences, researchers have studied the effect of conditions such as growth media and phase on Raman spectra. However, the majority of these studies focused on Gram-positive or Gram-negative bacteria. This article focuses on the influence of growth media and phase on Raman spectra and discrimination of mycobacteria, an acid-fast genus. Results showed that spectral differences from growth phase and media can be distinguished by spectral observation and multivariate analysis. Results were comparable to those found for other types of bacteria, such as Gram-positive and Gram-negative. In addition, the influence of growth phase and media had a significant impact on machine learning models and their resulting classification accuracy. This study highlights the need for machine learning models and their associated spectral libraries to account for various growth parameters and stages to further the transition of Raman spectral analysis of bacteria from laboratory to clinical settings. K E Y W O R D S discriminant analysis, environmental microbiology, mycobacteria, Raman spectroscopy

show abstract

Section: Resultssupporting

confidence: 92%

Effect of growth media and phase on Raman spectra and discrimination of mycobacteria

Hanson

Bishop

Barney

et al. 2019

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

“…For cDEP, the electrodes are not in contact with the analytes . To generate non‐uniform electric field for cell viability distinction, the device is comprised of main channel and side channel, and is also generally fabricated by the soft photolithography technique.…”

Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

View full text Add to dashboard Cite

Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

show abstract

“…A series of extracted mechanical properties of cells, such as deformation, viscoelasticity, and stiffness, are reported [42][43][44][45][46] by these two methods. Most importantly, DEP and ROT have a good prospect for use in the extraction of such dielectric properties of cells as membrane capacitance/conductance and cytoplasm conductivity/permittivity and, thereby, in bio-related applications including the identification of target cells and the detection of cell states [47][48][49][50][51][52][53]. Optically-induced dielectrophoreis (ODEP) [54][55][56], another DEP-based mechanism, is also proposed to extract the density and mass [57,58] as well as the electrical [59][60][61] and mechanical properties [62][63][64][65] of cells.…”

Section: Introductionmentioning

confidence: 99%

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Yang

Wang

et al. 2020

Micromachines

View full text Add to dashboard Cite

Cell dielectric properties, a type of intrinsic property of cells, can be used as electrophysiological biomarkers that offer a label-free way to characterize cell phenotypes and states, purify clinical samples, and identify target cancer cells. Here, we present a review of the determination of cell dielectric properties using alternating current (AC) electrokinetic-based microfluidic mechanisms, including electro-rotation (ROT) and dielectrophoresis (DEP). The review covers theoretically how ROT and DEP work to extract cell dielectric properties. We also dive into the details of differently structured ROT chips, followed by a discussion on the determination of cell dielectric properties and the use of these properties in bio-related applications. Additionally, the review offers a look at the future challenges facing the AC electrokinetic-based microfluidic platform in terms of acquiring cell dielectric parameters. Our conclusion is that this platform will bring biomedical and bioengineering sciences to the next level and ultimately achieve the shift from lab-oriented research to real-world applications.

show abstract

Simultaneous isolation and label‐free identification of bacteria using contactless dielectrophoresis and Raman spectroscopy

Cited by 21 publications

References 73 publications

Effect of growth media and phase on Raman spectra and discrimination of mycobacteria

Effect of growth media and phase on Raman spectra and discrimination of mycobacteria

Recent advances in dielectrophoresis‐based cell viability assessment

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Contact Info

Product

Resources

About