Recent advances in dielectrophoresis‐based cell viability assessment

Zhang, Junyan; Song, Zhenyu; Liu, Qinxin; Song, Yongxin

doi:10.1002/elps.201900340

Cited by 26 publications

(28 citation statements)

References 124 publications

(162 reference statements)

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“…As with other non-destructive and label-free approaches for measuring the number of viable cell in real-time [24,25], the LIM method is based on the metabolic activity of cells. Therefore, it is recommended that the power spectrum slope of the phase fluctuations be empirically determined for each cell type.…”

Section: Discussionmentioning

confidence: 99%

Label-Free Single Cell Viability Assay Using Laser Interference Microscopy

Beloglazova

Nikitiuk

Voronina

et al. 2021

Biology

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Laser interference microscopy (LIM) is a promising label-free method for single-cell research applicable to cell viability assessment in the studies of mammalian cells. This paper describes the development of a sensitive and reproducible method for assessing cell viability using LIM. The method, based on associated signal processing techniques, has been developed as a result of real-time investigation in phase thickness fluctuations of viable and non-viable MCF-7 cells, reflecting the presence and absence of their metabolic activity. As evinced by the values of the variable vc, this variable determines the viability of a cell only in the attached state (vc exceeds 20 nm2 for viable attached cells). The critical value of the power spectrum slope βc of the phase thickness fluctuations equals 1.00 for attached MCF-7 cells and 0.71 for suspended cells. The slope of the phase fluctuations’ power spectrum for MCF-7 cells was determined to exceed the threshold value of βc for a living cell, otherwise the cell is dead. The results evince the power spectrum slope as the most appropriate indicator of cell viability, while the integrated evaluation criterion (vc and βc values) can be used to assay the viability of attached cells.

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Section: Discussionmentioning

confidence: 99%

Label-Free Single Cell Viability Assay Using Laser Interference Microscopy

Beloglazova

Nikitiuk

Voronina

et al. 2021

Biology

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“…A list of publications and patents relating iDEP on cells for various applications are further shown in Tables S1 and S2 (Supplementary Materials) . While the use of iDEP for cells is well established, as evident by publications, patents, and commercial devices (Charlot Biosciences [ 31 ], Sandia National Laboratories [ 85 ]), more recent work has employed iDEP to manipulate and separate subcellular biological materials [ 10 , 11 , 18 , 86 ].…”

Section: Discussionmentioning

confidence: 99%

Insulator Based Dielectrophoresis: Micro, Nano, and Molecular Scale Biological Applications

Benhal

Quashie

Kim

et al. 2020

Sensors

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Insulator based dielectrophoresis (iDEP) is becoming increasingly important in emerging biomolecular applications, including particle purification, fractionation, and separation. Compared to conventional electrode-based dielectrophoresis (eDEP) techniques, iDEP has been demonstrated to have a higher degree of selectivity of biological samples while also being less biologically intrusive. Over the past two decades, substantial technological advances have been made, enabling iDEP to be applied from micro, to nano and molecular scales. Soft particles, including cell organelles, viruses, proteins, and nucleic acids, have been manipulated using iDEP, enabling the exploration of subnanometer biological interactions. Recent investigations using this technique have demonstrated a wide range of applications, including biomarker screening, protein folding analysis, and molecular sensing. Here, we review current state-of-art research on iDEP systems and highlight potential future work.

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“…Zhang et al. [40] discussed recent studies on the utilization of DEP for assessing cell viability. Henslee [41] reviewed a different method for determining the electrical properties of cell characterization including electrorotation and DEP‐based measurement.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

et al. 2021

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Dielectrophoresis (DEP) is a technique to manipulate trajectories of polarisable particles in nonuniform electric fields by utilizing unique dielectric properties. The manipulation of a cell using DEP has been demonstrated in various modes, thereby indicating potential applications in the biomedical field. In this review, recent DEP applications in the biomedical field are discussed. This review is intended to highlight research work that shows significant approach related to DEP application in biomedical field reported between 2016 and 2020. First, single‐shell model and multiple‐shell model of cells are introduced. Current device structures and recently introduced electrode patterns for DEP applications are discussed. Second, the biomedical uses of DEP in liquid biopsies, stem cell‐based therapies, and diagnosis of infectious diseases due to bacteria and viruses are presented. Finally, the challenges in DEP research are discussed, and the reported solutions are explained. DEP's potential research directions are mentioned.

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Recent advances in dielectrophoresis‐based cell viability assessment

Cited by 26 publications

References 124 publications

Label-Free Single Cell Viability Assay Using Laser Interference Microscopy

Label-Free Single Cell Viability Assay Using Laser Interference Microscopy

Insulator Based Dielectrophoresis: Micro, Nano, and Molecular Scale Biological Applications

Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

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