Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

Diabetes affects approximately 170 million people worldwide, is expected to double by 2030, and is a severe problem. Electrical stimulation (ES) via dielectrophoresis (DEP) technique may be an effective alternative in enhancing healing rates in diabetic patients with open ulcers. This research used DEP force (FDEP) to manipulate 3.2, 4.8, 10, and 15 μm polystyrene (PS) particles to predict the migration capability of human epidermal keratinocytes (HEKs). A numerical modeling method, MyDEP, was used to predict the interpretation of Clausius–Mossotti factors of PS particles and HEKs. The finite element method computes the electric field intensity and particle trajectory based on DEP and drag forces in their respective medium. DEP experiments on numerous size PS particles and alive HEKs were carried out in a tapered aluminium microelectrode array using a non‐uniform electric field. The distinct PS particles exhibit positive DEP (PDEP), crossover frequency (fXO), and negative DEP (NDEP), whereas the HEKs experience, only NDEP due to its high conductive medium in frequency ranges from 100 kHz to 1 MHz. Finally, the DIPP‐MotionV analysis shows that particle mobility between speed and acceleration is statistically considerable. When an appropriate frequency is applied to HEKs in random locations, the FDEP aligns at the desired target position based on its dielectric properties, which accelerates wound healing in in‐vivo conditions.

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“…P is the constant dipole moment vector, and E is the external electric field in Equation (1). [30][31][32]…”

Section: Dep Theorymentioning

confidence: 99%

“…The dielectric particle's geometry generally determines the proportional constant. P is the constant dipole moment vector, and E is the external electric field in Equation () 30–32

F_{italicDEP} = 2 π r^{3} ε_{0} ε_{m} Re [italicCMF] \nabla E^{2}

where ε m is the absolute permittivity of the surrounding medium, r is the particle radius.…”

Section: Dep Theorymentioning

confidence: 99%

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

Deivasigamani

Maidin

Nasir

et al. 2022

J of Applied Polymer Sci

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“…In another review, Pethig [ 15 ] described the current status of DEP’s theory, technology, and applications. Maidin et al analyzed the biomedical applications of DEP, especially in stem cell therapies, liquid biopsies, and infectious diseases [ 16 ]. Rahman et al also investigated the applications of DEP in biomedical science, including eukaryotic and prokaryotic cells, stem cells, oncology, and drug delivery [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation

et al. 2022

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Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics realizes the miniaturization of analytical devices by controlling the motion of a small volume of fluids in microchannels and microchambers. Accordingly, microfluidic devices have been widely used in particle/cell manipulation processes. Different microfluidic methods for particle separation include dielectrophoretic, magnetic, optical, acoustic, hydrodynamic, and chemical techniques. Dielectrophoresis (DEP) is a method for manipulating polarizable particles’ trajectories in non-uniform electric fields using unique dielectric characteristics. It provides several advantages for dealing with neutral bioparticles owing to its sensitivity, selectivity, and noninvasive nature. This review provides a detailed study on the signal-based DEP methods that use the applied signal parameters, including frequency, amplitude, phase, and shape for cell/particle separation and manipulation. Rather than employing complex channels or time-consuming fabrication procedures, these methods realize sorting and detecting the cells/particles by modifying the signal parameters while using a relatively simple device. In addition, these methods can significantly impact clinical diagnostics by making low-cost and rapid separation possible. We conclude the review by discussing the technical and biological challenges of DEP techniques and providing future perspectives in this field.

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“…The use of DEP technology to manipulate particle and cell motion has experienced great advancements in terms of versatility and flexibility and has been explored for decades (see e.g., [1,[4][5][6][7][8][9][10][11], more recently reviewed in [5,6,12]). The modern utilization of the DEP phenomenon focuses on the isolation, manipulation, and concentration of bioparticles using dielectrophoretic force in miniaturized microfluidic devices [6,13,14].…”

Section: Introductionmentioning

confidence: 99%

Direct In-Situ Capture, Separation and Visualization of Biological Particles with Fluid-Screen in the Context of Venus Life Finder Mission Concept Study

Weber

Petkowski²,

Weber

2022

Aerospace

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Evidence of chemical disequilibria and other anomalous observations in the Venusian atmosphere motivate the search for life within the planet’s temperate clouds. To find signs of a Venusian aerial biosphere, a dedicated astrobiological space mission is required. Venus Life Finder (VLF) missions encompass unique mission concepts with specialized instruments to search for habitability indicators, biosignatures and even life itself. A key in the search for life is direct capture, concentration and visualization of particles of biological potential. Here, we present a short overview of Fluid-Screen (FS) technology, a recent advancement in the dielectrophoretic (DEP) microbial particle capture, concentration and separation. Fluid-Screen is capable of capturing and separating biochemically diverse particles, including multicellular molds, eukaryotic cells, different species of bacteria and even viruses, based on particle dielectric properties. In this short communication, we discuss the possible implementation of Fluid-Screen in the context of the Venus Life Finder (VLF) missions, emphasizing the unique science output of the Fluid-Screen instrument. FS can be coupled with other highly sophisticated instruments such as an autofluorescence microscope or a laser desorption mass spectrometer (LDMS). We discuss possible configurations of Fluid-Screen that upon modification and testing, could be adapted for Venus. We discuss the unique science output of the Fluid-Screen technology that can capture biological particles in their native state and hold them in the focal plane of the microscope for the direct imaging of the captured material. We discuss the challenges for the proposed method posed by the concentrated sulfuric acid environment of Venus’ clouds. While Venus’ clouds are a particularly challenging environment, other bodies of the solar system, e.g., with liquid water present, might be especially suitable for Fluid-Screen application.

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Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

Cited by 17 publications

References 158 publications

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

A dielectrophoresis proof of concept of polystyrene particles and in‐vitro human epidermal keratinocytes migration for wound rejuvenation

Signal-Based Methods in Dielectrophoresis for Cell and Particle Separation

Direct In-Situ Capture, Separation and Visualization of Biological Particles with Fluid-Screen in the Context of Venus Life Finder Mission Concept Study

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