Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Yang, Xieliu; Wang, Junhai; Wang, Yuechao; Liu, Lianqing

doi:10.3390/mi11050513

Cited by 26 publications

(26 citation statements)

References 130 publications

(145 reference statements)

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“…ROT methods have recently been developed to improve single‐cell analysis and incorporate cell imaging and microfluidics [74–76]. These have incorporated 3D electrodes (Fig.…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

“…Improvements to ROT characterization through combining on‐chip analysis techniques, automation, and inclusion of cytoplasmic measurements have been shown [76–78]. Keim et al introduced an electrode array capable of simultaneous analysis of multiple‐ single cells.…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

“…Some of the biomedical applications of these cellular parameters are discussed later in this review as well as their values summarized in Table 1. However, for readers more interested in this technique, a recent review discusses ROT characterization as well [76].…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

“…Early experiments with this used the quadrupole electrode, castellated electrodes or other, 2D dimensional electrode configurations to elicit cell movement (Fig. 4B‐E) [46–48,76, 81,82]. In these studies, observations of crossover frequency as a function of medium conductivity were often fit to a simplified model of

K (ω)

to determine cell parameters.…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

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Review: Dielectrophoresis in cell characterization

Henslee

2020

Electrophoresis

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Many cellular functions are affected by and thus can be characterized by a cell's electrophysiology. This has also been found to correspond to other biophysical parameters such as cell morphology and mechanical properties. Dielectrophoresis (DEP) is an electrostatic technique which can be used to examine cellular biophysical parameters through the measuring of single or multiple cell response to electric field induced forces. This label‐free method offers many advantages in characterizing a cell population over conventional electrophysiology methods such as patch clamping; however, it has yet to see mainstream pharmacological application. Challenges such as the transdisciplinary nature of the field bridging engineering and the biological sciences, throughput, specificity as well as standardization are being addressed in current literature. This review focuses on the developments of DEP‐based cell electrophysiological characterization where determining cellular properties such as membrane conductance and capacitance, and cytoplasmic conductivity are the primary motivation. A brief theoretical review, techniques for obtaining these cell parameters, as well as the resulting cell parameters and their applications are included in this review. This review aims to further support the development of DEP‐based cell characterization as an important part of the future of DEP and electrophysiology research.

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“…ROT methods have recently been developed to improve single‐cell analysis and incorporate cell imaging and microfluidics [74–76]. These have incorporated 3D electrodes (Fig.…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

K (ω)

to determine cell parameters.…”

Section: Dep Characterization Methods and Parametersmentioning

confidence: 99%

See 2 more Smart Citations

Review: Dielectrophoresis in cell characterization

Henslee

2020

Electrophoresis

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“…Dielectrophoresis (DEP) has been used to detect bacteria [ 1 ], measure the electric properties of cells [ 2 , 3 ], and to form cell patterns [ 4 , 5 , 6 , 7 , 8 ] to apply to biomedical sciences [ 9 ]. Additionally, it has been especially studied as an external force for separating cells in microfluidic devices [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes

et al. 2020

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A microfluidic device is presented for the continuous separation of red blood cells (RBCs) and white blood cells (WBCs) in a label-free manner based on negative dielectrophoresis (n-DEP). An alteration of the electric field, generated by pairs of slanted electrodes (separators) that is fabricated by covering parts of single slanted electrodes with an insulating layer is used to separate cells by their sizes. The repulsive force of n-DEP formed by slanted electrodes prepared on both the top and bottom substrates led to the deflection of the cell flow in lateral directions. The presence of gaps covered with an insulating layer for the electric field on the electrodes allows the passing of RBCs through gaps, while relatively large WBCs (cultured cultured human acute monocytic leukemia cell line (THP-1 cells)) flowed along the slanted separator without passing through the gaps and arrived at an edge in the channel. The passage efficiency for RBCs through the gaps and the arrival efficiency for THP-1 cells to the upper edge in the channel were estimated and found to be 91% and 93%, respectively.

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A flow‐through microfluidic chip for continuous dielectrophoretic separation of viable and non‐viable human T‐cells

et al. 2021

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Effective methods for rapid sorting of cells according to their viability are critical in T cells based therapies to prevent any risk to patients. In this context, we present a novel microfluidic device that continuously separates viable and non-viable T-cells according to their dielectric properties. A dielectrophoresis (DEP) force is generated by an array of castellated microelectrodes embedded into a microfluidic channel with a single inlet and two outlets; cells subjected to positive DEP forces are drawn toward the electrodes array and leave from the top outlet, those subjected to negative DEP forces are repelled away from the electrodes and leave from the bottom outlet. Computational fluid dynamics is used to predict the device separation efficacy, according to the applied alternative current (AC) frequency, at which the cells move from/to a negative/positive DEP region and the ionic strength of the suspension medium. The model is used to support the design of the operational conditions, confirming a separation efficiency, in terms of purity, of 96% under an applied AC frequency of 1.5 × 10 6 Hz and a flow rate of 20 μl/h. This work represents the first example of effective continuous sorting of viable and non-viable human T-cells in a single-inlet microfluidic chip, paving the way for lab-on-a-chip applications at the point of need.

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Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Cited by 26 publications

References 130 publications

Review: Dielectrophoresis in cell characterization

Review: Dielectrophoresis in cell characterization

Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes

A flow‐through microfluidic chip for continuous dielectrophoretic separation of viable and non‐viable human T‐cells

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