Simultaneous enantioseparation and sensitivity enhancement of basic drugs using large‐volume sample stacking

Abstract

Simultaneous enantioseparation with sensitive detection of four basic drugs, namely methoxamine, metaproterenol, terbutaline and carvedilol, using a 20-mum ID capillary with native beta-CD as the chiral selector was demonstrated by the large-volume sample stacking method. The procedure included conventional sample loading either hydrodynamically or electrokinetically at longer injection times without polarity switching and EOF manipulation. In comparison to conventional injections, depending on the analyte, about several hundred- and a thousand-fold sensitivity enhancement was achieved with the hydrodynamic and the electrokinetic injections, respectively. The simple method developed was applied to the analysis of racemic analytes in serum samples and better recovery was achieved using hydrodynamic injection than electrokinetic injection.

Cyclodextrin‐modified MEKC for enantioseparation of hexaconazole, penconazole, and myclobutanil

“…Analysis time decreased from 17 to 14 min for the last peak of myclobutanil enantiomer when the temperature was increased from 25 to 408C. The results obtained in this work agree with those obtained by other authors [20,21], who have found that an increase in temperature causes a reduction in migration time because of the decrease in the viscosity of the buffer solution. However, an increase in the temperature up to 408C reduced the peak area, peak height, and peak efficiencies.…”

Stereoselective determination of drugs and metabolites in body fluids, tissues and microsomal preparations by capillary electrophoresis (2000–2010)

“…Furthermore, on-column preconcentration can also be attained by electrokinetic injection (for assay examples see Refs. [51,54,57,73,75,89,103,108,116,145]) and stacking procedures [49,71,75,79,87,100,108,116]. Electrophoretic mass transport is highly regulated, this allowing charged solutes to be concentrated (stacked) across an electrolyte discontinuity, including that produced initially between sample and running buffer.…”

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

“…Denola et al [77] reported the application of LVSS for sensitivity enhancement for the enantiomeric separation of four basic drugs (methoxamine, metaproterenol, terbutaline, and carvedilol) using b-CD as the chiral selector. Several 100-to 1000-fold enhancements in sensitivity were achieved with hydrodynamic and electrokinetic injections, respectively.…”