Simultaneous determination of the bioactive compounds from Sparassis crispa (Wulf.) by HPLC-DAD and their inhibitory effects on LPS-stimulated cytokine production in bone marrow-derived dendritic cell

Abstract

Sparassis crispa (Wulf.) belonging to the family of Sparassidaceae, has been widely used as an edible mushroom due to its unique flavor and functions to improve health. In this study, the compounds isolated from the extract of this mushroom were simultaneously quantified by the developed HPLC-DAD method and evaluated for the inhibitory activities on the production of the LPS-stimulated cytokines (IL-12p40, IL-6, and TNF-α) in bone marrow-derived dendritic cells (BMDCs). The contents of this compounds were 0.1928 ± 0.0118, 4.4137 ± 0.0240, 0.5237 ± 0.0005, 2.7303 ± 0.0206 mg/g for sparoside A (1), methyl 2,4-dihydroxy-3-methoxy-6-methylbenzoate (2), sparalide A (3), and 5'-deoxy-5'-methylthioadenosine (4), respectively, which demonstrated that they are the major constituents of this mushroom. Thus, our results were found that the sparoside A (1), 5-hydroxy-7-methoxyphthalide (6), 5-methoxy-7-hydroxyphthalide (7), nicotinamide (10), and adenosine (11) inhibit LPS-stimualted cytokine production, compound 6 is the most potent inhibitory activities on the production of IL-12p40, IL-6, and TNF-α with IC values of 0.19, 0.18, and 0.91 μM, respectively.

Rapid Characterization of Chemical Components in Edible Mushroom Sparassis crispa by UPLC-Orbitrap MS Analysis and Potential Inhibitory Effects on Allergic Rhinitis

“…Finally, it was confirmed directly for the first time that sparoside A showed obvious anti-inflammatory activity, which could dose-dependently decrease intracellular Ca 2+ mobilization with IC 50 value 5.06 ± 0.60 μM. This result was an important improvement over the previous data from Bang et al [12]. As to whether sparoside A could treat AR, further pharmacological studies are needed in the future.…”

“…However, due to the intrinsic complexity of chemical constitutes of S. crispa , few investigations have acquired a comprehensive configuration for the chemical profile of it. Bang et al [12] developed a HPLC-DAD method to simultaneously quantify the major compounds in extracts of S. crispa , but the upstream separation process was still unavoidable and laborious. Gas chromatography mass spectrometer (GC-MS) coupled with multivariate statistical analysis was performed by Seo et al [13] to understand the difference of metabolites between pileus and stipe of S. crispa , as well as the metabolic changes after fermentation by different microorganisms, which provided novel insights into chemical characteristics of different parts of this mushroom.…”

Ultrasonic-assisted enzymatic extraction of Sparassis crispa polysaccharides possessing protective ability against H2O2-induced oxidative damage in mouse hippocampal HT22 cells

“…Sparassis crispa (S. crispa), belonging to the family of Sparassidaceae, is also known as cauliower mushroom. 1 It is widely distributed in the Pacic Northwest, eastern Asia, and Australia and has been very popular among consumers because of its unique taste and nutritional values. 2,3 In recent years, S. crispa extracts have attracted more attention for their medicinal properties, such as antitumor, 4 hematopoietic, 5 anti-inammatory, 3 and hypocholesterolemic 6 effects.…”