Rapid Characterization of Chemical Components in Edible Mushroom Sparassis crispa by UPLC-Orbitrap MS Analysis and Potential Inhibitory Effects on Allergic Rhinitis

Abstract

Sparassis crispa is a kind of edible fungus widely grows in the north temperate zone, which shows various medicinal properties. Due to the complexity of chemical constitutes of this species, few investigations have acquired a comprehensive configuration for the chemical profile of it. In this study, a strategy based on ultra-high performance liquid chromatography (UPLC) combined with Orbitrap mass spectrometer (MS) was established for rapidly characterizing various chemical components in S. crispa. Through the summarized MS/MS fragmentation patterns of reference compounds and systematic identification strategy, a total of 110 components attributed to six categories were identified for the first time. Moreover, allergic rhinitis (AR) is a worldwide inflammatory disease seriously affecting human health, and the development of drugs to treat AR has been a topic of interest. It has been reported that the extracts of S. crispa showed obvious inhibitory effects on degranulation of mast cell- and allergen-induced IgE and proinflammatory mediators, but the active components and specific mechanism were still not clear. Src family kinases (SFKs) participate in the initial stage of allergy occurrence, which are considered the targets of AR treatment. Herein, on the basis of that self-built chemical database, virtual screening was applied to predict the potential SFKs inhibitors in S. crispa, using known crystal structures of Hck, Lyn, Fyn, and Syk as receptors, followed by the anti-inflammatory activity evaluation for screened hits by intracellular calcium mobilization assay. As results, sparoside A was directly confirmed to have strong anti-inflammatory activity with an IC50 value of 5.06 ± 0.60 μM. This study provides a useful elucidation for the chemical composition of S. crispa, and demonstrated its potential inhibitory effects on AR, which could promote the research and development of effective agents from natural resources.

Integration of ATAC-Seq and RNA-Seq Identifies Key Genes in Light-Induced Primordia Formation of Sparassis latifolia

“…Understanding the molecular mechanisms regulating the fruiting process in macro-fungi, especially in industrially cultivated mushrooms, has long been a goal in mycological research [28]. As a brown rot fungus, S. latifolia has exhibited various biological and pharmacological activities [16][17][18]. In China, the total fresh production of S. latifolia is over 20 tons/d.…”

“…S. latifolia was collected from Asian Sparassis [15], which has exhibited various biological and pharmacologic activities [16][17][18]. Based on the genome sequence of S. latifolia strains SP-C [19], researchers have identified hundreds of differentially expressed proteins during development [20].…”

Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC–Orbitrap–MS/MS Analysis

“…Recently, based on the highly efficient separation performance of ultra-high performance liquid chromatography (UPLC) and high sensitivity of mass spectrometry (MS), UPLC coupled with high-resolution mass spectrometry (HRMS) has become an important tool for characterization of chemical components in natural products [9]. Furthermore, a combination of UPLC separation with an Orbitrap MS system (UPLC-Orbitrap-MS/MS) has been widely used for screening and identification of chemical components in herbal medicines because of the advantages in terms of the peak capacity, resolution, separation time, and detection sensitivity [10][11][12].…”

Identification of lipophilic components in Citri Reticulatae Pericarpium cultivars by supercritical CO2 fluid extraction with ultra‐high‐performance liquid chromatography–Q Exactive Orbitrap tandem mass spectrometry

“…In addition to coumarins, seven other esters were also detected in the SFE extracts, including ferulic acid methyl ester, atractylenolide II, senkyunolide A, atractylenolide III, atractylenolide I, ligustilide, and 3‐butylidenephthalide. Under high‐energy collision, these compounds generate characteristic fragment ions by the removal of H 2 O, –C=O, and side‐chain alkyl groups [29].…”