Simultaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ratio spectra and multivariate calibration with model updating methods

Abstract

Background Metronidazole (MET) and Diloxanide Furoate (DF), act as antiprotozoal drugs, in their ternary mixtures with Mebeverine HCl (MEH), an effective antispasmodic drug. This work concerns with the development and validation of two simple, specific and cost effective methods mainly for simultaneous determination of the proposed ternary mixture. In addition, the developed multivariate calibration model has been updated to determine Metronidazole benzoate (METB) in its binary mixture with DF in Dimetrol ® suspension. Results Method (I) is the mean centering of ratio spectra spectrophotometric method (MCR) that depends on using the mean centered ratio spectra in two successive steps that eliminates the derivative steps and therefore the signal to noise ratio is enhanced. The developed MCR method has been successfully applied for determination of MET, DF and MEH in different laboratory prepared mixtures and in tablets. Method (II) is the partial least square (PLS) multivariate calibration method that has been optimized for determination of MET, DF and MEH in Dimetrol ® tablets and by updating the developed model, it has been successfully used for prediction of binary mixtures of DF and Metronidazole Benzoate ester (METB) in Dimetrol ® suspension with good accuracy and precision without reconstruction of the calibration set. Conclusion The developed methods have been validated; accuracy, precision and specificity were found to be within the acceptable limits. Moreover results obtained by the suggested methods showed no significant difference when compared with those obtained by reported methods. Graphical Abstract

Different spectrophotometric and TLC-densitometric methods for determination of olmesartan medoxomil and hydrochlorothiazide and their degradation products

“…It has a lot of advantages like, high productivity with minimum cost, improved precision and truthful of results, increased affirmation for carrying out laboratory operations, easier validation of the variable steps of an analytical technique [46]. Wide applications of multivariate calibretion methods were seen for different multi-components mixtures as in [47][48][49][50][51][52].…”

Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture

“…The developed MCR method depends on the mean centering of ratio spectra, it eliminates the derivative steps and therefore signal-tonoise ratio is enhanced [35]. It has been applied for resolving binary and ternary mixtures in complex samples with unknown matrices [35]. The mathematical explanation of the method was illustrated by Afkhami and Bahram [36][37][38].…”

Rapid Separation and Determination of Metronidazole Benzoate and Other Antiprotozoal Drugs by Pressurized Capillary Electrochromatography

“…In addition to spectrophotometry [6][7][8][9], electrochemistry, voltammetry [10], and thin-layer chromatography [11], the methods commonly used for the separation and determination of MB or MB-mixed other compounds in various matrices, such as blood plasma, urine, drug, and aqueous solution samples, were high-performance liquid chromatography (HPLC) [12][13][14][15][16][17] and ultra performance liquid chromatography (UPLC) [18] using highly or totally aqueous mobile phases combined with nonwetted nonpolar packed columns (such as C 8 and C 18 ) and gradient elution mode, which can lead to a number of issues such as bubble formation. ey are also approach-complicated and timeconsuming.…”