Simultaneous determination of seven taxoids in rat plasma by UPLC–MS/MS and pharmacokinetic study after oral administration of Taxus yunnanensis extracts

A simple and efficient sample preparation for taxanes in Taxus chinensis needles with natural menthol‐based aqueous deep eutectic solvent

“…After a period of clinical application, it has been found that as an active ingredient of a medicinal plant, there were a series of problems in the development of paclitaxel drugs, such as paclitaxel production is still very far away to meet the needs for clinical applications. Numerous similar compounds of paclitaxel have been discovered, including 10‐deacetylbaccatin III (10‐DAB III), baccatine III, 7‐xylosyl‐10‐deacetyltaxol (7‐xyl‐10‐DAT), 7‐epi‐10‐deacetyltaxol (7‐epi‐10‐DAT), 10‐deacetyltaxol (10‐DAT), and cephalomannine [7,8]. Moreover, these taxanes have become the key factor in the field of paclitaxel research in recent years.…”

An ultrasensitive LC–MS/MS method with liquid phase extraction to determine paclitaxel in both cell culture medium and lysate promising quantification of drug nanocarriers release in vitro

“…The reported method obtained a recovery that does not exceed 70% despite its very ultrasensitive PTx quantification (2 pg/mL) [20]. In addition, most of the reported methods applied for PTx extraction in plasma, urine, feces and tissue matrices, use non-polar organic solvents such as methyl tert-butyl ether, diethyl ether, dichloromethane, mixture of ether/chloroform and chlorobutane/ACN [20][21][22][23]27]. It is known that PTx is more soluble in methanol than in chloroform, in ether, in dichloromethane or in ACN.…”

“…Indeed, this technique not only removes potential interferents but also allows concentrating the targeted analytes by solvent evaporation. This process is one of the most commonly used approaches for liquid biological sample preparation [23,27,28,30]. Rapid and simple treatment steps were applied to samples to optimize the extraction process such as proteins precipitation, methanol extraction and cell lysis.…”

“…Indeed, due to its high hydrophobicity, most of in vitro studies of PTx release from nanocarriers were performed in simulated physiological medium supplemented with surfactant Tween 80 [11][12][13][14][15][16][17][18]. Numerous analytical methods using liquid phase extraction and solid phase extraction combined to the LC-MS/MS technique were developed for ultrasensitive quantifi- cation of PTx in biological samples such as cells, plasma, urine, feces or tissues [19][20][21][22][23][24][25][26][27][28][29][30]. Nevertheless, most of the published works use high-performance liquid chromatography or spectrophotometric UV-vis absorbance [11][12][13][14][15]17] and radio labeled [16] detection to determine the in vitro profile of PTx release from nanocarriers.…”

Antineoplastic drugs and their analysis: a state of the art review

“…11 and Table 6, respectively. Most methods for taxanes analysis are based on RPLC coupled to MS 305,309,[426][427][428][429][430][431][432][433][434][435][436][437][438][439][440][441][442] and UV detection. 138,269,[443][444][445][446][447][448][449][450][451][452][453] LODs lower than 0.05 ng mL −1 were reached for the analysis of docetaxel in blood using a column-switching method involving a preconcentration step on a trapping column, prior to LC-MS analysis.…”