A simple and efficient sample preparation for taxanes in Taxus chinensis needles with natural menthol‐based aqueous deep eutectic solvent

Abstract

Taxanes are natural anticancer constituents, and the sample preparation from matrix normally depends on organochlorine solvents. In this study, green and natural menthol-based aqueous deep eutectic solvent was synthesized and used for sample preparation for taxanes. Five key parameters were optimized and the optimal preparation conditions were as follows: menthol/1-propanol ratio 1:1 (mol/mol), solid-liquid ratio 1:30 g/mL, extraction time 30 min, ultrasonic power 250 W, and water content 80%. Under the above conditions, the total extraction efficiency of seven main taxanes was 1.25-to 1.44-fold to the conventional methods. In addition, a high-performance liquid chromatography method with C 18 column was established for quantitation of seven main taxanes in <25 min, which had excellent linearity (R 2 > 0.9986), precision (relative standard deviation < 3.00%), repeatability (relative standard deviation < 3.69%), and recovery (90.26-109.00%). This method performed the extraction, and enrichment processes simultaneously, and it had advantages such as high extraction efficiency, simple operation, low cost, and eco-friendliness. This work indicated that the natural menthol-based deep eutectic solvent aqueous could be an excellent alternative to the sample preparation from Taxus or other plants. K E Y W O R D S aqueous deep eutectic

Optimization of Extraction of Bioactive Compounds from Baphicacanthus cusia Leaves by Hydrophobic Deep Eutectic Solvents

“…The carbonyl band from the lactic acid was originally located at low wave number values (1721.20 cm −1 ) and it broadened and shifted to the highest values (1724.25 cm −1 ) in the L-Men-Lac. A similar phenomenon has been reported for DESs consisting of menthol and carboxylic acids or alcohols [32,33,38].…”

“…So the extraction of the three target compounds was suitable for hydrophobic DES extraction. Following methods used in previous reports [32,33], we selected six organic acids and alcohols that can form eutectic mixtures with L-menthol as components of DESs. These compounds include acetic acid, lactic acid, levulinic acid, n-propanol, isopropanol and tert-butanol, as shown in Table 1.…”

“…Some new DESs that are based on the combination of menthol with alcohols and organic acids have been synthesized. These DESs showed a high degree of hydrophobicity, which indicated that menthol was a good candidate for the preparation of hydrophobic DESs [32,33]. Menthol can be extracted from plants in the genus mentha and has a wide range of sources.…”

New phases for analytical scale extraction from plants: Current and future trends

“…It normally requires the mixing of two or three components under magnetic stirring, and keeping the temperature at 40-90°C until a homogeneous liquid is produced. [36,38,[41][42][43][44]47]. When preparing a DES, it is important that hydrogen bonds are formed between the two components (HBA and HBD), and an FT-IR spectrum of the mixture can therefore be a useful tool to evaluate their correct formation [42,47].…”

Green and enhanced extraction of coumarins from Cortex Fraxini by ultrasound‐assisted deep eutectic solvent extraction

“…DESs were prepared by using the heating method. Accurately weighed raw materials were added in a glass flask and stirred in a water bath at 80 • C to generate homogeneous liquid, and a proper amount of water was added during DES preparation as reported [18][19][20][21][22]. The produced DESs contain 17 groups, and the whole list of the abbreviations of prepared DESs is displayed in Table 1.…”