Simultaneous determination of rivanol and mifepristone in human plasma by a HPLC-UV method with solid-phase extraction

Guo, Zhiyong; Wei, Danyi; Yin, Guochuan; Wang, Sui; Zhao, Shasha; Chu, Yen-Ping; Zhai, Jinxia

doi:10.1016/j.jchromb.2007.06.016

Cited by 11 publications

(11 citation statements)

References 12 publications

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“…Repeating the extraction and increasing the proportion of diethyl ether to plasma further increased the recovery (p<0.001) and maximal extraction efficiencies were achieved using either triple extractions with ratios of 5:1 or 10: 1 or double extraction with diethyl ether at a ratio 20:1, the last being selected. These recoveries were similar to those reported using solid-phase extraction [8;9;11]. Under these conditions the recovery of alfaxolone was 92% which was therefore suitable for further validation as an internal standard.…”

Section: Resultssupporting

confidence: 86%

“…This limit of quantitation was lower than those reported previously (0.5ng [12], 300pg - 1ng on column [10;11]). Accordingly the analysis could be performed using only 100μl of plasma, in contrast to volumes of approximately 0.5-1 ml in previous reports [9-11]. In development, it was found that an alternative steroidal drug, spironolactone could also be used as suitable internal standards, yielding linear responses.…”

Section: Resultsmentioning

confidence: 99%

“…Previous methods of quantitative analysis of RU38486 have included radioimmunoassay [7], however the antibody used exhibited substantial cross-reactivity with the drug’s metabolites. More recently the approach of HPLC analysis with UV detection has provided highly accurate and precise measurements, with suitable limits of detection to support its conventional clinical use, following solid phase extraction of plasma [8;9]. Guo et al attained limits of detection and quantitation of 6 and 10ng/ml (0.6 and 1ng on column) respectively, using norethisterone as the internal standard [10], whereas Stith et al developed a method of analysis of RU38486 in coyote ( Canis latrans ) serum [11], achieving a linear range of detection in the range 10 -1000ng/ml (5-500ng on column) using a synthetic analog of RU38486, RTI-3021-003, as the internal standard.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Homer

Reynolds

Mattsson

et al. 2009

Journal of Chromatography B

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A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430→372; m/z 333→297 respectively). Quantification was linear over the range 0.5-500ng (r 2 >0.997), precise and accurate (intra-assay RSD ≤7.2%, RME ≤8.2%; interassay RSD ≤15.7% RME ≤10.2%). The limit of quantification was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Homer

Reynolds

Mattsson

et al. 2009

Journal of Chromatography B

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“…In order to select optimum conditions for preparation of MCNs, the effects of Cs concentration, TPP concentration, and Cs/MIF mass ratio on the EE and DL of nanoparticles were investigated [25]. Because the pH values have great influence on the properties of MIF [27], the pH value of the TPP solution was also optimized.…”

Section: Resultsmentioning

confidence: 99%

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Zhang¹,

Wu²,

Li³

et al. 2016

Beilstein J. Nanotechnol.

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In addition to its well-known abortifacient effect, mifepristone (MIF) has been used as an anticancer drug for various cancers in many studies with an in-depth understanding of the mechanism of action. However, application of MIF is limited by its poor water solubility and low oral bioavailability. In this work, we developed a drug delivery system based on chitosan nanoparticles (CNs) to improve its bioavailability and anticancer activity. The MIF-loaded chitosan nanoparticles (MCNs) were prepared by convenient ionic gelation techniques between chitosan (Cs) and tripolyphosphate (TPP). The preparation conditions, including Cs concentration, TPP concentration, Cs/MIF mass ratio, and pH value of the TPP solution, were optimized to gain better encapsulation efficiency (EE) and drug loading capacity (DL). MCNs prepared with the optimum conditions resulted in spherical particles with an average size of 200 nm. FTIR and XRD spectra verified that MIF was successfully encapsulated in CNs. The EE and DL of MCNs determined by HPLC were 86.6% and 43.3%, respectively. The in vitro release kinetics demonstrated that MIF was released from CNs in a sustained-release manner. Compared with free MIF, MCNs demonstrated increased anticancer activity in several cancer cell lines. Pharmacokinetic studies in male rats that were orally administered MCNs showed a 3.2-fold increase in the area under the curve from 0 to 24 h compared with free MIF. These results demonstrated that MCNs could be developed as a potential delivery system for MIF to improve its anticancer activity and bioavailability.

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“…* email: varutmarius@yahoo.com, phone: +4 0761762069 detection limit can be reduced to ppb range as it is the case for quinine [5]. Some research teams reported simultaneous detection of ethacridine and mifepristone in human plasma by HPLC-UV [6].…”

mentioning

confidence: 99%

Method of Determination of Rivanol by Laser Induced Fluoroscence

Palarie¹,

Văruţ²,

Chirigiu³

2019

Rev. Chim.

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In this paper has been developed a new method for determination of Ethacridine lactate (rivanol) by laser induced fluorescence (LIF). The method has been used in order to avoid the possibility of error induced in determination of aqueous solution of rivanol by spectrophotometric method due to the shift of absorption band to short wavelength with decreasing of the concentration. The new method allows the determination of rivanol at low concentrations, in the range of 1 mM.

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Simultaneous determination of rivanol and mifepristone in human plasma by a HPLC-UV method with solid-phase extraction

Cited by 11 publications

References 12 publications

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Method of Determination of Rivanol by Laser Induced Fluoroscence

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