Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry

Abstract

A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430→372; m/z 333→297 respectively). Quantification was linear over the range 0.5-500ng (r 2 >0.997), precise and accurate (intra-assay RSD ≤7.2%, RME ≤8.2%; interassay RSD ≤15.7% RME ≤10.2%). The limit of quantification was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

A validated HPLC-MS/MS method for the quantification of systemic mifepristone after subcutaneous application in mice

“…Several complex sample preparation procedures have been described for the extraction of MIF and derived metabolites from plasma, which require larger sample volumes, which were not feasible in our preclinical setting. 24,25 To keep sample preparation as straightforward as possible, we used a liquidliquid extraction (LLE) protocol with diethylether as the extraction solvent for plasma and tissue homogenates, as described earlier by Homer et al 26 The data obtained with LLE and described herein resulted in reliable detection of the analytes, and, therefore, solid phase extraction was omitted.…”

Analytical Method Development and Validation for Estimation of Mifepristone in Pure and Pharmaceutical Dosage Form

“…It inhibits the growth of secretory endometrium, which leads to the production of menstruation [6].Mifepristone has antiglucocorticoid and anti-androgenic properties when combined with glucocorticoid and androgen receptors [7][8][9].The use of mifepristone in the first trimester of pregnancy further leads to abortion. It is also used as a post-coital contraceptive [8][9][10][11]. The mechanism of action of Mifepristone is presented in:…”

Determination of cymipristone in human plasma by liquid chromatography–electrospray ionization-tandem mass spectrometry

“…1). It was proposed that cymipristone had a different metabolic profile as opposed to mifepristone when used in humans [6]. However, methods of determination of cymipristone in biological fluids have never been reported.…”

“…However, methods of determination of cymipristone in biological fluids have never been reported. Earlier publications have described methods for the analysis of mifepristone by LC-MS/MS [6,7]. In this paper, a new liquid chromatography-electrospray ionization-tandem mass spectrometry method using mifepristone as the internal standard (IS) was established and validated, and it was successfully applied to a pharmacokinetic study of cymipristone in healthy Chinese female subjects.…”