Simultaneous determination of phenol, cresol, xylenol isomers and napthols in urine by capillary gas chromatography

Bieniek, G

doi:10.1016/0378-4347(96)00104-1

Cited by 35 publications

(19 citation statements)

References 16 publications

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“…The naphthols were released from the kieselgel by treatment with 1 ml of ethanol. The gas chromatographic (GC) conditions were the same as previously described for the urine analysis (19). The detection limit was 3 pglm3.…”

Section: Naphthol Analysismentioning

confidence: 99%

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Urinary naphthols as an indicator of exposure to naphthalene

Bieniek¹

1997

Scand J Work Environ Health

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Section: Naphthol Analysismentioning

confidence: 99%

“…The samples were transferred to the analytical laboratory and kept frozen until the analysis. The GCIFID analyses for 1-naphthol and 2-naphthol in urine were carried out as described earlier (19). The 1-and 2-naphthol concentrations were corrected for creatinine or specific gravity of 1.024.…”

Section: Urine Sampling and Analysismentioning

confidence: 99%

Urinary naphthols as an indicator of exposure to naphthalene

Bieniek¹

1997

Scand J Work Environ Health

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“…In the linear range, it is possible to quantify this metabolite in samples containing o-cresol levels below or above the biological limit of exposure of 0.50 mg L -1 , 12 thus being satisfactory for most situations of occupational exposure to the solvent. When using liquid-liquid extraction, the linear range reported by others researchers were: 0.2 to 10 mg L -1 , 1 5 to 100 mg L -1 , 19 and 50 to 500 mg L -1 . 28 The detection and quantification limits of the proposed method were 0.05 and 0.10 mg L -1 , respectively.…”

Section: Analytical Figures Of Meritmentioning

confidence: 88%

Analysis of ortho-cresol in urine by solid phase microextraction-capillary gas chromatography

Paiva

Martins²,

Siqueira³

2007

J. Braz. Chem. Soc.

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Neste trabalho foi desenvolvido um método usando a microextração em fase sólida (SPME) para análise cromatográfica em fase gasosa capilar com detector de ionização por chama (CG/ IC) de orto-cresol urinário, metabólito do tolueno. Após otimização das variáveis da SPME e da validação do método, foram analisadas amostras de urina de 27 trabalhadores expostos a solventes em oficinas de reparo automotivo. As melhores condições de extração foram obtidas com a hidrólise ácida da urina, extração em fibra de carbowax/divinilbenzeno (CW/DVB) 70 μm por 20 min em pH neutro, com adição de 3 g de Na 2 SO 4 , sob agitação. O método mostrou linearidade entre 0,1 e 1,5 mg L -1 de o-cresol em urina, LOQ de 0,1 mg L -1 , repetibilidade entre 6,8 e 7,8%. O valor médio de o-cresol em urina dos trabalhadores foi de 0,35 ± 0,23 mg L -1 . O método SPME/CG/DIC mostrou ser rápido, simples e aplicável para fins de biomonitorização de trabalhadores expostos.The object of the present study was the development of method for the determination of urinary ortho-cresol using solid-phase microextraction (SPME), followed by capillary gas chromatography-flame ionization detection (GC/FID). After optimization of the SPME variables and validation of the method, it was applied to analysis of o-cresol in urine collected from 27 workers exposed to solvents in automotive repair shops. The maximum extraction efficiency was obtained using fiber of carbowax-divinylbenzene (CW/DVB), 70 μm, immerged 20 min in acid hydrolysed urine added with 3 g Na 2 SO 4 at pH 7.0 and under magnetic stirring. The method showed linearity between 0.1 to 1.5 mg L -1 , LOQ of 0.1 mg L -1 , precision between 6.8 to 7.8% CV. Mean o-cresol in urine from exposed workers was 0.35 ± 0.23 mg L -1 . The SPME-GC/FID method is fast, simple and can be applied to the occupational toluene biomonitoring.

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“…Urinary metabolites were identified by GC/MS after enzymatic hydrolysis 7,16) , solidphase extraction on styrene-divinylbenzene resin and after a derivatization procedure 17) . The GC/FID analyses of 1-naphthol, 2-naphthol, 2-methylphenol, 4-methylphenol, 2,4-and 2,5-dimethylphenol, 3,4-dimethylphenol and 3,5-dimethylphenol were carried out as previously described 11) . The concentrations of metabolites in urine were corrected against creatinine.…”

Section: Air Samplingmentioning

confidence: 99%

“…In environmental and toxicological analyses, chromatographic methods, such as gas-chromatography (GC) or high-performance liquid chromatography (HPLC), play a major role in the detection of chemicals in many materials such as: air [2][3][4] , cigarette smoke 5) , blood samples 6) and urine 2,[7][8][9][10][11] . Hoshika and Murayama 12) performed a simultaneous analysis of lower fatty acids, phenols and indoles in feces.…”

mentioning

confidence: 99%

Occupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography‐Mass Spectrometry Method

Bieniek

Kurkiewicz

Wilczok

2004

Journal of Occupational Health

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University of Silesia, Poland-A method for the qualitative analysis of aromatic hydrocarbons in air and their various urinary metabolites is presented. The air was sampled in charcoal tubes and extracted with carbon disulfide. The hydrocarbons were identified as being aliphatic hydrocarbons (C 9 -C 19 ), aromatic hydrocarbons and heterocyclic compounds. The urinary metabolites after enzymatic hydrolysis were analyzed by solid-phase extraction with a styrened i v i n y l b e n z e n e r e s i n , s i l y l a t i o n w i t h N , Obis(trimethylsilyl)acetamide and GC/MS for separation and detection. Satisfactory separation of all compounds investigated was achieved without interference due to matrix peaks. The following compounds were identified in the urine of workers: dimethylphenol isomers, 4-ethyl-1,3-benzenediol, 2-ethoxybenzoic acid and methoxyphenols. Trimethylsilyl d e r i v a t i v e s o f a r o m a t i c h y d r o x y a c i d s a n d hydroxymethoxyacids were found in the urine of occupationally exposed workers by means of a silylation procedure.

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Simultaneous determination of phenol, cresol, xylenol isomers and napthols in urine by capillary gas chromatography

Cited by 35 publications

References 16 publications

Urinary naphthols as an indicator of exposure to naphthalene

Urinary naphthols as an indicator of exposure to naphthalene

Analysis of ortho-cresol in urine by solid phase microextraction-capillary gas chromatography

Occupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography‐Mass Spectrometry Method

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