Simultaneous Determination of Oxalic and Tartaric Acid With Chemiluminescence Detection

Abstract

A time-resolved chemiluminescence method for the determination of oxalic and tartaric acid was developed. The method is based on the kinetic distinction of the chemiluminescent reaction of Ru(bipy) 3 2+ and oxalic and tartaric acid with Ce IV . The oxalic acid system gives the highest chemiluminescence intensity at 2 s, whereas the tartaric acid system gives its most intense chemiluminescence emission at 50 s. Oxalic acid (1.4 3 10 25 -1.4 3 10 27 mol l 21 ) and tartaric acid (1.5 3 10 24 -2.9 3 10 26 mol l 21 ) in synthetic urine were determined simultaneously with RSDs of better than 5% (n = 5). The detection limits of oxalic acid and tartaric acid were 2.7 310 28 and 2.7 3 10 26 mol l 21 , respectively. The detailed chemiluminecence mechanism for the over-all process is discussed. The kinetic distinction between the oxalic and tartaric acid systems was elucidated.

Development of a Direct Chemiluminescence Method for the Determination of Nucleic Acids Based upon Their Reaction with Cerium(IV) in the Presence of Rutheniumtrisdipyridine

“…A 0.4 ml portion of 1.0×10 -5 g/ml nucleic acids and 0.4 ml of 3.6×10 -5 g/ml, Ru(bipy) 3 Br 2 were mixed in a sample cuvette, and then transferred to the measuring chamber held at a constant temperature of 298 K. After the start button had been pushed, 0.2 ml of 1.0 mmol/l Ce(SO 4 ) 2 (0.1 mol/l H 2 SO 4 ) was automatically injected into the reaction cuvette and the produced CL was measured at the maximum intensity. Calibration graphs of the emission intensity versus the concentration of nucleic acids were constructed.…”

“…The CL kinetic characteristics of nucleic acids, Ru(bipy) 3 2+ and Ce(IV) in sulfuric acid medium were studied in detail. The rate of reaction of each nucleic acid in solution was shown to be different: the peak time was at 3 s for fish sperm DNA, 5 s for calf thymus DNA and 7 s for yeast RNA (Fig.…”

“…Thermally denatured DNA was prepared by submerging the volumetric flask containing DNA solution in boiling water for 15 min, and then chilling in ice water for 10 min to prevent the renaturation of DNA. A 1.2 mg/ml stock solution of Ru(bipy) 3 Br 2 (prepared in our laboratory 2 ) was prepared by dissolving a required amount of the salt in water and diluting with water to 1000 ml.…”

“…In this research, it was discovered that chemiluminescence was generated during oxidation of Ru(bipy) 3 2+ by cerium(IV) in a sulfuric acid medium. The emission intensity and the sensitivity were greatly enhanced when nucleic acids were also present in reaction system.…”

Chemiluminescence Determination of Sulfite in Sugar and Sulfur Dioxide in Air Using Ru(bipy)32+-K2S2O8 System

“…It can be applied to the determination of 6-mercaptopurine 23 in alkaline media and oxalic, tartaric acids etc. 24,25 in sulfuric acid media.…”