Simultaneous Determination of Ofloxacin, Tetrahydrozoline Hydrochloride, and Prednisolone Acetate by High-Performance Liquid Chromatography

Abstract

A simple, specific, and precise high-performance liquid chromatographic method has been developed for the simultaneous determination of ofloxacin (OFX), tetrahydrozoline hydrochloride (THC), and prednisolone acetate (PAC) in ophthalmic suspension using propylparaben (POP) as the internal standard. The mobile phase consists of 0.05 M phosphate buffer-acetonitrile (65:35, v/v), and the pH is adjusted to 2.7 with orthophosphoric acid. A column containing octadecyl silane chemically bonded to porous silica particles (Waters Spherisorb, 5 microm ODS 1, 4.6 x 150 mm) is used as the stationary phase. The detection is carried out using a variable wavelength UV-vis detector set at 210 nm for OFX and THC and 254 nm for POP (internal standard) and PAC. The solutions are chromatographed at a constant flow rate of 1.2 mL/min. Retention times for OFX, THC, POP, and PAC are approximately 2.5, 4.5, 7.8, and 9.5 min, respectively. The relative retention times are approximately 0.14 min for OFX, 0.35 min for THC, 1.00 min for POP, and 1.22 min for PAC. The linearity range and percent recoveries for OFX, THC, and PAC are 24-120, 4-16, and 16-80 microg/mL and 100.48%, 100.34%, and 100.21%, respectively.

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…Allylestrenol-α-tocopherol tablets, 102 beclomethasone dipropionate inhalers 103 and other formulations (St), 104 betamethasone, its acetate and other corticosteroids in ointments, 105,106 betamethasone and dexamethasone (delicate separation problem! ), 107,108 betamethasone-diclofenac sodium-cyanocobalamine tablet, 109 ethinylestradiol-norgestrel tablet, 114 ethinylestradiollevonorgestrel and ethinylestradiol-gestodene tablets, 117 finasteride tablet (St), 118 fluocortolone pivalate and hexanoate suppositories, 119 hydrocortisone-oxytetracycline-nystatin in pharmaceutical preparations, 120 hydrocortisone acetate and hemisuccinate-lidocaine in pharmaceutical preparations, 121 hydrocortisone acetate-methyl-and propylparaben topical cream (St), 122 hydrocortisone sodium phosphate gel (St), 123 nestorone implants (St), 124 pancuronium bromide injection (St), 125 prednisolone acetate-tetrahydrozoline hydrochlorideofloxacin ophthalmic preparations, 126 prednisolone sodium phosphate implantable infusion pump (St), 127 progesterone in micellar systems and other formulations, 128 triamcinolone, its acetate and acetonide and several other corticosteroids (St), 129 triamcinolone acetonide dermatological patches, 130 triamcinolone acetonide, methyl-and propylparaben topical cream (St). 131 Carefully validated stability-indicating HPLC methods were used for the determination of impurities and degradants in bulk pipecuronium bromide, 132 budesonide, 133 aqueous spironolactone-β-cyclodextrin solutions, 134 and for the photodegradation kinetic study of danazol.…”

Computation of geometric representation of novel spectrophotometric methods used for the analysis of minor components in pharmaceutical preparations

“…For mixture (A), HPLC methods [37,38] and spectrophotometric methods [39] were reported for its analysis, where the later used standard TZH addition to the dosage form and then the claimed concentration of TZH in the preparation was calculated after subtraction of the added concentration; Meanwhile, no methods have been reported for the analysis of mixture (B).…”

Simultaneous Determination of Ciprofloxacin Hydrochloride and Dexamethasone Sodium Phosphate in Eye Drops by HPLC

“…A few methods have also been described for the simultaneous determination of Dexamethasone with other drugs such as Chloremphenicol 6 , ofloxacin 7,8,9 . A gradient reverse phase HPLC method 10 has been reported in the literature for simultaneous determination of CFH and DSP using a nucleosil C18 column with a mixture of mobile phase A consisted of 50 milli molar citric acid and potassium phosphate buffer.…”