Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Gǒrög, Sándor

doi:10.2116/analsci.20.767

Cited by 51 publications

(18 citation statements)

References 242 publications

(198 reference statements)

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“…For quality control in drug analysis, the simplest and fastest method is the most desirable. According to Görög, the predominant method for the assay of steroid drugs is reversed phase high performance liquid chromatography (RP-HPLC) with UV detection (Görög, 2004). However, spectrophotometric methods are also widely used because they are inexpensive and easy to perform.…”

Section: Resultsmentioning

confidence: 99%

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms

Scremin

Piazzon

Silva

et al. 2010

Braz. J. Pharm. Sci.

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(DFZ) is a glucocorticoid used as an anti-inflammatory and immunosuppressant drug. No official methods are available for DFZ determination in pharmaceutical formulations. The objective of this study was to develop, validate and compare spectrophotometric (UV and colorimetric) and highperformance liquid chromatography (HPLC) methods, for the quantitative determination of DFZ in tablets and oral suspension. For the UV method, ethanol was used as the solvent, with detection at 244 nm. The colorimetric method was based on the redox reaction with blue tetrazolium in alkaline medium, with detection at 524 nm. The method by HPLC was carried out using a C 18 column, mobile phase consisting of acetonitrile:water (80:20, v/v) with a flow rate of 1.0 mL min -1 and detection at 244 nm. The methods proved linear (r > 0.999), precise (RSD < 5%) and accurate (recovery > 97%). Statistical analysis of the results indicated that the UV and HPLC methods were statistically equivalent, while the values obtained for the colorimetric method differed significantly from the other methods. O deflazacorte (DFZ) é um fármaco glicocorticóide usado como antiinflamatório e imunossupressor. Métodos oficiais não estão disponíveis para a determinação de DFZ em formas farmacêuticas. Este estudo teve como objetivo desenvolver, validar e comparar métodos por espectrofotometria (UV e colorimetria) e cromatografia líquida de alta eficiência (CLAE), na determinação quantitativa de DFZ em comprimidos e suspensão oral. O método por UV utilizou etanol como solvente, com detecção em 244 nm. O método colorimétrico foi baseado na reação de redução com azul de tetrazólio em meio alcalino, com detecção em 524 nm. O método por CLAE utilizou coluna C 18 ; fase móvel constituída de acetonitrila:água (80:20, v/v), com fluxo de 1,0 mL min -1 e detecção em 244 nm. Os métodos foram lineares (r > 0,999); precisos (RSD < 5 %), e exatos (recuperação > 97%). As análises estatísticas dos resultados obtidos indicaram que os métodos por UV e por CLAE foram estatisticamente equivalentes, enquanto os valores obtidos para o método colorimétrico diferiram significativamente dos demais métodos.Unitermos: Deflazacorte/determinação quantitativa. Cromatografia líquida de alta eficiência/análise quantitativa. Espectrofotometria/análise quantitativa. Medicamentos/análise quantitativa.

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Section: Resultsmentioning

confidence: 99%

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms

Scremin

Piazzon

Silva

et al. 2010

Braz. J. Pharm. Sci.

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“…Despite their potential utility to elucidate molecular tracers in complex mixtures, these methods are applied to more specific samples such as mixtures containing high molecular weight (HMW) compounds (e. g., lipids and bitumen waxes [70 -73], drugs and hormones [74,75], and biopolymers [e. g., 76,77]). …”

Section: Other Methodsmentioning

confidence: 99%

Gas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research

Medeiros

Simoneit

2007

J of Separation Science

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ReviewGas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research Gas chromatography, especially when coupled with mass spectrometry, is the analytical method of choice for elucidation of biomarker compounds present in organic mixtures extracted from geological, environmental, and biological samples. This review describes the biomarker concept, i. e., the precursor natural products to the geological/environmental derivatives, and their application as multi-tracers in the geosphere and ambient environment. The mass spectrometric methods currently utilized for such analyses are reviewed with a general key to the literature, and typical examples of applications using GC -MS are also described.

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“…As described in Görög's review article [10], it is the predominant method in the United States Pharmacopoeia for assay and related impurities tests of bulk steroid hormones and hormone-like structures. In the overwhelming majority of cases, porous octadecylsilica support (3-10 m) is used for the reversed-phase separations.…”

Section: Hplc Methods Developmentmentioning

confidence: 99%

Chromatographic analysis and decomposition product characterization of compound SR16157 (NSC 732011)

Wang

ChunYANG

Wang

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Cited by 51 publications

References 242 publications

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms

Spectrophotometric and HPLC determination of deflazacort in pharmaceutical dosage forms

Gas chromatography coupled to mass spectrometry for analyses of organic compounds and biomarkers as tracers for geological, environmental, and forensic research

Chromatographic analysis and decomposition product characterization of compound SR16157 (NSC 732011)

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