Simultaneous determination of noradrenaline and dopamine in <i>Portulaca oleracea </i>L. by capillary zone electrophoresis

Yue, Mei-E; Jiang, Tianyue; Shi, Yan‐Ping

doi:10.1002/jssc.200400045

Cited by 42 publications

(22 citation statements)

References 14 publications

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“…Capillary electrophoresis (CE) has the advantages of excellent separation efficiency, rapid analysis and minimal use of samples and solvents for complex samples. It is an alternative technique for the separation and detection of several species of pharmaceutical importance that are extracted and used in traditional Chinese herbal medicine (Jiang et al, 2003;Yue et al, 2004Yue et al, , 2005.…”

Section: Introductionmentioning

confidence: 99%

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Yue

Shi

2007

Biomedical Chromatography

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The simultaneous determination of 3,4-dimethoxy-3'-hydroxy propiophenone, 5-hydroxy-7-methoxy coumarin, 7-hydroxy coumarin, 3',5'-dimethoxy apigenin, apigenin and 4-hydroxy cinnamic acid in the extract of S. katochaete has been investigated by capillary electrophoresis for the first time. The six active components were completely separated within 10 min in 20 mM Na(2)HPO(4) buffer at pH 11.00 with 10% (v/v) methanol and detected at 214 nm. The applied voltage was 15 kV and the temperature was kept at 25 degrees C. The effects of buffer pH, the concentration of Na(2)HPO(4) and the concentration of methanol on the separation efficiency were studied systematically. The regression equations revealed good linear relationships (correlation coefficients were 0.9987-0.9998) between the peak area of each analyte and its concentration. The relative standard deviations (RSD) of migration time and peak areas were <1.63 and <4.03%, respectively. The application of this method for the separation and determination of the six bioactive components in S. katochaete was reported. The contents of the six analytes ranged from 0.023 to 0.131 mg/g and recoveries ranged from 94.7 to 104.8%.

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Section: Introductionmentioning

confidence: 99%

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Yue

Shi

2007

Biomedical Chromatography

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“…There are also valid documents for the presence of biologically active catecholamines including noradrenaline, dopamine and L-dopa in P. oleracea. Large concentrations of noradrenaline in the fresh plant have been reported as 2.5 mg/g which is probably larger than that of extractable amount from the mammalians suprarenal glands (22). These neurotransmitters may be responsible for increasing blood pressure in this model of hypertension in which dexamethasone intensifies the reactivity to vasopressor effects of catecholamines.…”

Section: Discussionmentioning

confidence: 88%

Portulaca oleracea seeds extract does not prevent dexamethasone-induced hypertension in rats

Safaeian¹,

Baniahmad²,

Esfandiari³

et al. 2017

J Herbmed Pharmacol

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The results of this study revealed that hydroalcoholic extract of P. oleracea seeds aggregates hypertension in dexamethasoneinduced hypertensive rats. Therefore, it is recommended that its using be avoided in hypertensive patients receiving glucocorticoids. Please cite this paper as: Introduction:Portulaca oleracea is used as a nutritional and medicinal plant. The aim of this study was to assess the effect of hydroalcoholic extract of P. oleracea seeds in dexamethasone -induced hypertension in rats. Methods: For induction of hypertension, dexamethasone (30 µg/kg/d, subcutaneously) was administered for 14 days. Animals received P. oleracea extract as a pretreatment at various doses of 100, 200 and 400 mg/kg/d orally from 4 days before dexamethasone administration and during the test period. Systolic blood pressure (SBP) and heart rate were measured using tailcuff method. The weight of thymus gland was estimated as a marker of glucocorticoid activity. Results: Dexamethasone injection significantly increased SBP (P < 0.001) while decreased the body and thymus weights (P < 0.05 and P < 0.001, respectively). Oral administration of P. oleracea could not prevent rising in SBP and decreasing in thymus weight. It also increased heart rate in hypertensive rats at the dose of 400 mg/kg/d (P < 0.05). Conclusion:The results of this study revealed that hydroalcoholic extract of P. oleracea seeds aggregates hypertension in dexamethasone-induced hypertensive rats. Hence, it should be used with caution in hypertensive patients receiving glucocorticoids.

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“…Generally, at equal molarities, oligoprotic buffers like citrate will give substantially higher currents than monoprotic species such as acetate. Therefore, type and concentration of the buffer for CZE should be optimized to obtain a good resolution of the interested compounds in a satisfactory time [40,42,43,48,58,63,[87][88][89][90][91][92][93][94][95][96][97][98].…”

Section: Buffer Type and Concentrationmentioning

confidence: 99%

“…Methanol, ACN, 1-propanol and 2-propanol can be used to improve the selectivity and resolution of CZE by improving both the separation and the peak shape [101,102], increasing the solubility [48,64], changing the migration time [50,91,103,104] and/or decreasing the adsorption and diffusion [88] of analytes. Another way of manipulating EOF and reducing analyte interaction with the silica wall is to add amines to the buffer.…”

Section: Organic Additivesmentioning

confidence: 99%

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Optimization of CZE for analysis of phytochemical bioactive compounds

Wang

2006

Electrophoresis

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Advantages of CZE such as high efficiency, low cost, short analysis time, and easy implementation result in its wide applications for analysis of phytochemical bioactive compounds (e.g. flavonoids, alkaloids, terpenoids, phenolic acid, saponins, anthraquinones and coumarins). However, several aspects, including sample preparation, separation, and detection have significant effects on CZE analysis. Therefore, optimization of these procedures is necessary for development of the method. In this review, sample preparation such as extraction method and preconcentration, separation factors including buffer type, concentration and pH, additives, voltage and temperature, as well as detection, e.g. direct and indirect UV detection, LIF and MS were discussed for optimization of CZE analysis on phytochemical bioactive compounds. The optimized strategies were also reviewed.

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Simultaneous determination of noradrenaline and dopamine in Portulaca oleracea L. by capillary zone electrophoresis

Cited by 42 publications

References 14 publications

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Portulaca oleracea seeds extract does not prevent dexamethasone-induced hypertension in rats

Optimization of CZE for analysis of phytochemical bioactive compounds

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