Optimization of CZE for analysis of phytochemical bioactive compounds

Abstract

Advantages of CZE such as high efficiency, low cost, short analysis time, and easy implementation result in its wide applications for analysis of phytochemical bioactive compounds (e.g. flavonoids, alkaloids, terpenoids, phenolic acid, saponins, anthraquinones and coumarins). However, several aspects, including sample preparation, separation, and detection have significant effects on CZE analysis. Therefore, optimization of these procedures is necessary for development of the method. In this review, sample preparation such as extraction method and preconcentration, separation factors including buffer type, concentration and pH, additives, voltage and temperature, as well as detection, e.g. direct and indirect UV detection, LIF and MS were discussed for optimization of CZE analysis on phytochemical bioactive compounds. The optimized strategies were also reviewed.

Current trends in isolation, separation, determination and identification of isoflavones: A review

“…More details of electrophoretic techniques and their applications in the analyses of isoflavones (and also other flavonoid substances) can be found in recent papers by Herrero et al [59] and Li et al [60].…”

Recent Applications of Capillary Electrophoresis in the Analysis of Traditional Chinese Medicines

“…Several other features of CZE such as high resolution, speed of method development, costs, etc., also enable its successful application in complex TCM analysis. Method optimization of CZE for analysis of phytochemical bioactive compounds has been reviewed in the past [7]. In this review some applications of CZE in TCM analysis will be emphasized ( Table 1).…”

“…Some formats of CE such as capillary zone electrophoresis (CZE) [7], micellar electrokinetic chromatography (MEKC) [8], non-aqueous CE (NACE) [9] and capillary electrochromatography (CEC) [10], providing different principals of separation, have been widely applied for the analysis of TCMs. This review will summarize the recent development of CE in the analysis of TCMs covering mainly the papers published in the past five years (2005)(2006)(2007)(2008)(2009), and applications were sorted by different CE separation formats.…”

Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

“…The seeds of Datura metel under investigation had been carried aboard a retrievable satellite and exposed to extraterrestrial space, but effects of the space environment (weightlessness and ionising radiation) on the contents of atropine and scopolamine were non-significant. Optimised procedures for CZE for analysis of phytochemical bioactive compounds were summarised [150]. Recently, CE was used to identify mixtures of toxic alkaloids, among them atropine, scopolamine, and anisodamine, in human blood and urine [151].…”