Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

Aehle, Elke; Dräger, Birgit

doi:10.1016/j.jchromb.2010.03.007

Cited by 55 publications

(33 citation statements)

References 173 publications

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“…Chromatographic conditions like injector temperature (240, 260, 280, and 300 C), ion source temperature (180, 200, 220, and 240 C), interface temperature (280, 290, 300, and 310 C), initial (60, 80, 100, and 120 C) and final (280, 290, 300, and 310 C) column temperature, as well as the column temperature rate (10,20,30, and 40 C/min), the initial temperature hold time (0.0, 0.5, 1.0, and 1.5 min), and the carrier gas flow rate (0.6, 0.8, 1.0, and 1.2 ml/min) were also optimized. The optimal conditions are in bold.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

“…[3] On the other hand, in case of carbamate poisoning, the use of oximes is controversial and considered contraindicated, [7] so the recommended treatment is the combination of atropine and diazepam. [10] The necessity for the investigation of the cause of the adverse effects that appear during atropine administration led to the development of analytical methods for atropine determination in biological fluids. [3] Atropine is the initial drug of choice in acute organophosphate poisoning and the only antidotal therapy in carbamate poisoning, due to its efficacy for their parasympathomimetic effects.…”

Section: Introductionmentioning

confidence: 99%

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A simple and sensitive GC/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples

Papoutsis

Νικολάου

Spiliopoulou

et al. 2011

Drug Testing and Analysis

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Atropine is used in the daily clinical practice for the treatment of poisonings caused by anticholinesterase pesticides, due to its sympathomimetic action. The investigation of the cause of the adverse effects that appear during atropine administration showed the necessity for the development and validation of a simple, rapid, sensitive, and specific method for the determination of atropine levels in serum samples. The developed method includes liquid-liquid extraction with ethyl acetate: dichloromethane (3:1, v/v) and derivatization using N,O-bis(trimethylsilyl)-trifluoracetamide (BSTFA) with 1% trimethylchlorsilane (TMCS) in acetonitrile environment. The method was found to be selective, linear, accurate, and precise according to international guidelines. The recovery was higher than 85.9%, the limit of quantification was 2.00 ng/ml, and the calibration curve was linear within the range of 2.00-500 ng/ml (R(2) ≥ 0.992). Accuracy and precision were also calculated and were found to be less than 5.2 and 8.7%, respectively. The developed method was applied in a real case of accidental poisoning with chlorpyrifos in order to determine the atropine serum levels of the patient. The proposed method proved to be useful for the investigation of adverse effects that appear during atropine treatment of patients poisoned by anticholinesterase pesticides and it can also be used for the investigation of poisonings caused after consumption of atropine containing plants.

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Section: Methods Development and Optimizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A simple and sensitive GC/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples

Papoutsis

Νικολάου

Spiliopoulou

et al. 2011

Drug Testing and Analysis

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“…Moreover, it cannot be used for ground materials and compound feeds. In these instances GC-MS and LC-MS/MS methods can be applied (Dräger 2002;Aehle & Dräger 2010). Limits of detection (LODs) of these chromatographic methods are generally in the low µg kg -1 range for individual compounds, such as the multi-analyte LC-MS/MS method reported by Jandrić et al (2011) that included several TAs.…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

Intra- and inter-laboratory validation of a dipstick immunoassay for the detection of tropane alkaloids hyoscyamine and scopolamine in animal feed

Mulder

Holst

Nivarlet³

et al. 2014

Food Additives & Contaminants: Part A

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Tropane alkaloids (TAs) are toxic secondary metabolites produced by plants of, inter alia, the genera Datura (thorn apple) and Atropa (deadly nightshade). The most relevant TAs are (-)-L-hyoscyamine and (-)-L-scopolamine, which act as antagonists of acetylcholine muscarinic receptors and can induce a variety of distinct toxic syndromes in mammals (anti-cholinergic poisoning). The European Union has regulated the presence of seeds of Datura sp. in animal feeds, specifying that the content should not exceed 1000 mg kg(-1) (Directive 2002/32/EC). For materials that have not been ground, visual screening methods are often used to comply with these regulations, but these cannot be used for ground materials and compound feeds. Immunological assays, preferably in dipstick format, can be a simple and cost-effective approach to monitor feedstuffs in an HACCP setting in control laboratories. So far no reports have been published on immunoassays that are capable of detecting both hyoscyamine and scopolamine with equal sensitivity and that can be used, preferably in dipstick format, for application as a fast screening tool in feed analysis. This study presents the results obtained for the in-house and inter-laboratory validation of a dipstick immunoassay for the detection of hyoscyamine and scopolamine in animal feed. The target level was set at 800 µg kg(-1) for the sum of both alkaloids. By using a representative set of compound feeds during validation and a robust study design, a reliable impression of the relevant characteristics of the assay could be obtained. The dipstick test displayed similar sensitivity towards the two alkaloids and it could be concluded that the test has a very low probability of producing a false-positive result at blank level or a false-negative result at target level. The assay can be used for monitoring of TAs in feedstuffs, but has also potential as a quick screening tool in food- or feed-related poisonings.

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“…The plot shows an upsurge trend indicative of the increased interest and activity. Sixty eight percent (68%) of these publications came out in 2006–2010, the coverage of this review, with the following topics: pharmaceutical and herbal products applications 16–49, food and food contaminants 50–88, detection 89–103, toxicological compounds 104–111, microchips 67, 88, 112, and preconcentration 113–120. There were reviews on the general advances and new aspects of CE‐natural products analysis 15, 121, 122.…”

Section: Previous Reviewsmentioning

confidence: 99%

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

2011

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An extensive search of the published research and review articles indicated a growing number of works that employed electroseparation techniques. Capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC), in particular, were found to be popular for the analyses of natural products. This review outlines the important developments in this field over the years, especially during the 2006-2010 period. An overview of the natural product applications such as pharmaceuticals/herbal products, fingerprinting and quality control, food and food contaminants, and toxicological compounds of interest to forensics were presented. Important areas of detection strategies, microchips, sample preconcentration, and chiral separations were also discussed. Hence, quick information was provided on the researches already undertaken and the possibilities of unexplored areas. In addition, fundamental concepts for the understanding of CZE and EKC and their suitability for natural products analyses were briefly discussed.

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Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

Cited by 55 publications

References 173 publications

A simple and sensitive GC/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples

A simple and sensitive GC/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples

Intra- and inter-laboratory validation of a dipstick immunoassay for the detection of tropane alkaloids hyoscyamine and scopolamine in animal feed

Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)

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