Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis

Abstract

The simultaneous determination of 3,4-dimethoxy-3'-hydroxy propiophenone, 5-hydroxy-7-methoxy coumarin, 7-hydroxy coumarin, 3',5'-dimethoxy apigenin, apigenin and 4-hydroxy cinnamic acid in the extract of S. katochaete has been investigated by capillary electrophoresis for the first time. The six active components were completely separated within 10 min in 20 mM Na(2)HPO(4) buffer at pH 11.00 with 10% (v/v) methanol and detected at 214 nm. The applied voltage was 15 kV and the temperature was kept at 25 degrees C. The effects of buffer pH, the concentration of Na(2)HPO(4) and the concentration of methanol on the separation efficiency were studied systematically. The regression equations revealed good linear relationships (correlation coefficients were 0.9987-0.9998) between the peak area of each analyte and its concentration. The relative standard deviations (RSD) of migration time and peak areas were <1.63 and <4.03%, respectively. The application of this method for the separation and determination of the six bioactive components in S. katochaete was reported. The contents of the six analytes ranged from 0.023 to 0.131 mg/g and recoveries ranged from 94.7 to 104.8%.

&lt;sup&gt;13&lt;/sup&gt;C-NMR Data from Coumarins from Moraceae Family

“…Table 3 lists the 13 C NMR data from the Moraceae family isolated furocoumarins. This kind of coumarin is the one that appears most often in the studied species, highlighting the linear furocoumarins such as psoralen (27). In this case, the signals to C-2' (δ C 147.0) and C-3' (δ C 106.6) evidence the presence of methine carbons typical from furan ring.…”

“…The compounds with the highest number of occurrences in the family are the linear furocoumarins, especially the psoralen (27) and the bergapten (26), which together with the simple coumarin umbelliferone (2), appear in four from the six genera mentioned.…”

“…This paper reports a literature review, describing the data of 13 C NMR from 53 coumarins isolated from the Moraceae family. From this group, several appear repeatedly in different species, such as psoralen (27), for example. Table 1 …”

Determination of organic acids by CE and CEC methods

“…To achieve a sensitive analysis of the selected solutes even in samples containing only very low concentrations of organic acids, a large-volume stacking strategy and samples prepared in low-conductive matrices containing 50% ACN had to be used. Bioactive compounds, among them several substances with carboxylic acid functionalities have been determined in different plant materials [56][57][58][59][60]. Six bioactive compounds including 4-hydroxycinnamic acid have been analyzed in Saussurea katochaete by Yue et al [56].…”

“…Table 2 gives an overview of applications describing the determination of organic material in a variety of natural samples. Those come from quite different fields such as the analysis of low molecular mass organic acids in plant extracts [52][53][54] or rubber latex [55] as well as the identification of bioactive carboxylic acids with more complex molecular structures in plant material suitable for medical purposes [56][57][58][59][60]. Three studies on the content of small organic acids in plant and leaf extracts, employing quite similar CE conditions namely electrolytes with a strongly UV-absorbing coion for indirect detection and an EOF modifier for coelectroosmotic conditions, were conducted by Rivasseau et al [52], Warren et al [53], and Wang et al [54].…”