Simultaneous determination of natural and synthetic estrogens by EKC using a novel microemulsion

Abstract

A novel microemulsion based on sodium bis(2-ethylhexyl) sulfosuccinate (AOT) was developed for the simultaneous determination of natural and synthetic estrogens by microemulsion EKC (MEEKC). The microemulsion system consisted of 1.4% w/w AOT, 1.0% w/w octane, 7.0% w/w 1-butanol and 90.6% w/w 20 mM sodium salt of 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO) and 10 mM phosphate buffer at pH 12.5. A baseline resolution in the separation of estrone, 17beta-estradiol, estriol, estradiol 17-hemisuccinate, etinilestradiol, estradiol 3-benzoate, and estradiol 17-valerate was achieved in comparison to the traditional MEEKC system with SDS in less than 15 min. The optimized electrophoretic conditions included the use of an uncoated-silica capillary of 60 cm of total length and 75 microm id, an applied voltage of 25 kV, a temperature of 25 degrees C and 214 UV-detection. Parameters of validation such as specificity, linearity, accuracy, LOD, LOQ and robustness were evaluated according to international guidelines. Due to its simplicity, accuracy, and reliability, the proposed method can be an advantageous alternative to the traditional methodologies for the analysis of natural and synthetic estrogens in different pharmaceutical forms.

Application of surfactants and microemulsions to the extraction of pyrene and phenanthrene from soil with three different extraction methods

“…In this research, 1-butanol was selected as the cosurfactant because it is the most commonly used organic solvent in the microemulsion [37]. Fig.…”

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

“…In the course of a systematic optimization study a heptane-1-butanol-Brij 76-sodium taurodeoxycholate microemulsion in a phosphate buffer containing ␤-cyclodextrin was found suitable for the separation of the components of the mixture of cortisone, hydrocortisone, prednisolone and their acetates and this method was used for the purity check of these drugs [114]. In other studies for the separation of corticosteroids the microemulsion contained 1-butanol, SDS and diethyl l tartrate [115] and for estrogens octane-1-butanol-sodium bis(2-ethylhexyl)sulfosuccinate-sodium 3-cyclohexylamino-2-hydroxy-1-propane sulfonate [116].…”

Advanced Protocols in Oxidative Stress III

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