Simultaneous determination of methamphetamine and ketamine in human hair using ultrasonication and LC-ESI-MS/MS method

Abstract

A rapid and reliable method for simultaneous determination of methamphetamine (MAM) and ketamine (KET) in human hair was developed. MAM and KET in hair samples were collected using ultrasonication with methanol/5 M hydrochloric acid (HCl) for 1 hour, and detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this experiment, drug-free human hairs were added into standard solution and kept for 16 hours in order to minimize the difference between blank spiked samples and authentic samples. D8-MAM as internal standard, the linear ranges of MAM and KET were 1-100 pg mg À1 and 2-200 pg mg À1 with correlation coefficients greater than 0.999, respectively. The limit of detection (S/N $ 3) was 0.03 pg mg À1 for MAM and 0.06 pg mg À1 for KET, the limit of quantification was (S/N $ 10) 0.09 pg mg À1 for MAM and 0.21 pg mg À1 for KET. Accuracy evaluated by recovery spiked at three different concentrations, was in the ranges of 91-114% for MAM and 88-119% for KET.The intra-and inter-day standard deviations of all analysis results were less than 3.50%. This method was applied to analyze residue levels of MAM and KET in human hair samples.

Graphene oxide assisted electromembrane extraction with gas chromatography for the determination of methamphetamine as a model analyte in hair and urine samples

“…The proposed method is superior in many ways such as linear range and LOD over other reported methods either using voltammetric, GC–MS or HPLC–UV and derivatization methods . Furthermore, several LC–MS methods have been reported for determination of METH in the recent years . Although, LC–MS based methods can create high sensitivity but this instrument is very expensive and not accessible as a routine instrument.…”

Simultaneous Determination of Cortisol, Cortisone, and Multiple Illicit Drugs in Hair among Female Drug Addicts with LC-MS/MS

“…The illegal consumption of illicit drugs causes a series of serious physiological and psychological health problems [ 1 , 2 ]. MAM and MDMA, the prevalent drugs of abuse in China [ 3 ], have stimulant effects on central nervous system [ 4 , 5 ].…”

“…In most previous studies, main ionization coupled to LC-MS/MS for determination of illicit drugs or 6-AM in hair was electrospray ionization (ESI) [ 2 , 3 , 24 , 26 ]. Unfortunately, none of these present studies can simultaneously detect of ketamine and heroin.…”

Current status of hair analysis in forensic toxicology in China

“…The t re atment of hair, gas chromatographymass spectrometer (GCMS) and liquid chromatography tandemmass spectrometry (LCMS/MS) analysis were developed and optimized in scientific research. During the past 30 years, the detection of anabolic steroids in black hair, the drug concentrationtime relationship in hair and a kine tics model were investigated; also, the identification and evaluation system of psychoactive substances in hair was established [8][9][10][11][12][13][14][15][16][17][18][19][20]. Then, information related to whether the subject took the drug or not, the type of drug and the drug ingestion history were provided in casework in judicial practice.…”